中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (13): 2081-2086.doi: 10.3969/j.issn.2095-4344.0505

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

脂肪干细胞与羟基磷灰石/β-磷酸三钙复合体修复兔椎体缺损

王腾飞，宋兴华，麦麦提艾力•阿不力克木，陈江涛，陶 颖，杨 勇   

  1. 新疆医科大学第一附属医院骨科中心，新疆维吾尔自治区乌鲁木齐市 830054
  • 修回日期:2018-03-31 出版日期:2018-05-08 发布日期:2018-05-08
  • 通讯作者: 宋兴华，博士，主任医师，教授，新疆医科大学第一附属医院骨科中心，新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:王腾飞，男，1988年生，河南省南阳市人，蒙古族，新疆医科大学在读硕士，主要从事骨病、骨肿瘤研究。
  • 基金资助:

    国家自然科学基金(81360283)

Hydroxyapatite/beta-tricalcium phosphate scaffolds combined with adipose-derived stem cells for the treatment of spinal defects in rabbits

Wang Teng-fei, Song Xing-hua, Maimaitiaili Abulikemu, Chen Jiang-tao, Tao Ying, Yang Yong   

  1. Department of Orthopedics, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Revised:2018-03-31 Online:2018-05-08 Published:2018-05-08
  • Contact: Song Xing-hua, M.D., Chief physician, Professor, Department of Orthopedics, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Wang Teng-fei, Master candidate, Department of Orthopedics, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81360283

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摘要：

文章快速阅读：

文题释义：
椎体缺损：多见于脊柱结核及脊柱肿瘤行病灶清除术后所造成的缺损，这样的骨缺损一般较大，易导致椎体塌陷，稳定性下降，加重骨关节退变，因此需要在椎体缺损区域进行植骨填补，以支撑脊柱。骨组织工程的快速发展，为其治疗提供了新的思路，对缺损骨组织进行修复和重建，能一定程度上恢复脊柱后凸、侧凸的矫正率，维持脊柱的力学稳定性，促进病灶的愈合。
兔脂肪干细胞/羟基磷灰石/β-磷酸三钙复合体：脂肪干细胞是存在于脂肪组织中的一种特殊的干细胞，其在一定条件下能诱导成为骨细胞。羟基磷灰石化学成分、晶体结构及物理性能与人体骨骼中磷灰石相似，且钙磷摩尔比与天然骨接近。羟基磷灰石与β-磷酸三钙构成的复合材料是目前广泛应用于临床的人工复合材料，不仅具有较好的生物相容性、可降解性及骨传导性，还可以诱导骨细胞生长，促进骨组织的生成，而且具有良好的孔隙率，有利于种子细胞与材料复合，是骨组织工程中比较理想的支架材料。

 

摘要
背景：骨缺损的修复不仅是临床上的难题，也一直是骨科领域的热点。虽然自体骨移植是骨修复技术的“金标准”，但由于自体骨来源有限，易出现取骨区感染、疼痛等症状，而且同种异体骨有免疫排斥、愈合缓慢、感染等特点，因此研发新型骨缺埙修复材料迫在眉睫。
目的：评价兔脂肪干细胞与羟基磷灰石/β-磷酸三钙骨组织工程复合体修复兔椎体缺损的效果。
方法：取3月龄新西兰大白兔38只，其中2只用于体外培养脂肪干细胞，将第3代经成骨诱导分化的兔脂肪干细胞接种于羟基磷灰石/β-磷酸三钙支架上进行体外培养2周。36只新西兰大白兔随机分为3组(n=12)，均在L4/5椎体前缘制备一直径约5 mm、深约3 mm椎体缺损，A组、B组分别植入兔脂肪干细胞/羟基磷灰石/β-磷酸三钙复合体、羟基磷灰石/β-磷酸三钙支架，C组不植入材料。术后4，8，12周行脊柱正侧位DR照射，进行Lane-Sandhu X射线评分，术后12周处死取标本，行大体观察、组织病理学观察椎体缺损修复情况。
结果与结论：①大体观察发现A组椎体缺损区基本被新生骨组织取代，修复效果明显优于B组及C组；②术后12周时，A组椎体缺损区材料基本吸收，B组椎体缺损材料部分吸收，可见骨痂形成，C组椎体缺损区边界尚清晰，可见片状钙化影。经Lane-Sandhu X射线评分后统计学分析，A组椎体缺损修复情况明显优于B组及C组(P < 0.05)；③组织病理学观察发现A组材料吸收明显，B组部分材料残留，仅见到部分纤维性骨痂及少许类骨样组织形成，C组可见大量纤维组织及少许骨痂形成；④以上结果表明，脂肪干细胞与羟基磷灰石/β-磷酸三钙构建的骨组织工程复合体具有较好的修复椎体缺损的能力。

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0001-6248-8935(王腾飞)

关键词: 脂肪干细胞, 羟基磷灰石/β-磷酸三钙, 椎体缺损, 干细胞, 国家自然科学基金

Abstract:

BACKGROUND: Repair of bone defects is not only a clinical problem, but also a hot topic in the field of orthopedics. Although autologous bone grafting is considered as the “gold standard” for bone repair, its use is limited due to the limited source of autogenous bone, bone infections and pains that are easy to occur in the donor region. Allograft bones are always associated with immune rejection, slow healing, and infection. Therefore, it is imminent to develop new materials for bone repair.
OBJECTIVE: To explore the effect of rabbit adipose-derived stem cells (rADSCs) as seed cells and hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) composite as a carrier on the repair of rabbit vertebral defects.
METHODS: Thirty-eight 3-month-old New Zealand white rabbits were selected, and two of them were used to culture rADSCs in vitro. Passage 3 rADSCc were inoculated on HA/β-TCP scaffolds and then cultured in vitro for 2 weeks. A 5 mm×5 mm×3 mm bone defect was prepared at the anterior edge of L4/5 vertebral body in the remaining 36 rabbits. These model rabbits were then randomized into cell-scaffold composite group, scaffold group and control group with no intervention, with 12 rabbits in each group. rADSCs/HA/β-TCP composite and HA/β-TCP scaffold were implanted into the cell-scaffold and HA/β-TCP groups, respectively. Anteroposterior and lateral DR of the spine and Lane-Sandhu X-ray were performed at 4, 8, 12 postoperative weeks. All rabbits were sacrificed at 12 postoperative weeks and specimens were collected for gross and histopathological observations.
RESULTS AND CONCLUSION: Under the gross observation, bone defects in the cell-scaffold group were essentially replaced by new bone tissues, which was significantly better than that in the scaffold group and control group. At 12 postoperative weeks, the material implanted was basically absorbed in the cell-scaffold group, partially absorbed in the scaffold group and poorly absorbed in the control group in which there was a clear boundary with the surrounding tissues and patchy calcified shadows were visible. X-ray results showed that the repair effect in the cell-scaffold group was better than that in the scaffold group and control group (P < 0.05). Histopathological findings showed the marked absorption of the implant in the cell-scaffold group, partial residual in the scaffold group with some fibrous calluses and osteoid tissues, and a large amount of fiber tissues and a small amount of calluses in the control group. Overall, the rADSCs/HA/β-TCA has a good ability to repair bone defects.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Spinal Injuries, Adipose Tissue, Stem Cells, Hydroxyapatites, Calcium Phosphates, Tissue Engineering

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