中国组织工程研究

中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (21): 3367-3373.doi: 10.3969/j.issn.2095-4344.2014.21.016

• 药物控释材料 drug delivery materials • 上一篇    下一篇

胶原/生物活性玻璃/壳聚糖增强型复合支架

孟永春1，陈晓峰1，2，南开辉1，李玉莉2，罗小刚1，邓春林2   

  1. 1温州市生物材料工程技术研究中心，温州医学院附属眼视光医院，浙江省温州市  325027；2国家人体组织功能重建工程技术研究中心，华南理工大学材料科学与工程学院，广东省广州市  510640
  • 出版日期:2014-05-21 发布日期:2014-05-21
  • 通讯作者: 邓春林，国家人体组织功能重建工程技术研究中心，华南理工大学材料科学与工程学院，广东省广州市 510640
  • 作者简介:孟永春，温州市生物材料工程技术研究中心，温州医学院附属眼视光医院，浙江省温州市 325027
  • 基金资助:

    浙江省自然科学基金(Y407241)；国家自然科学基金(51072055)；国家自然科学基金重点项目(50830101)；国家重点基础研究发展计划(973计划)(2011CB606204)资助

Collagen/bioactive glass/chitosan composite scaffolds

Meng Yong-chun1, Chen Xiao-feng 1, 2 , Nan Kai-hui1, Li Yu-li2, Luo Xiao-gang1, Deng Chun-lin2   

  1. 1 Wenzhou Engineering Research Center for Biomaterials, the Eye Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China; 2 National Engineering Research Center for Tissue Restoration and Reconstruction, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong Province, China
  • Online:2014-05-21 Published:2014-05-21
  • Contact: Deng Chun-lin, National Engineering Research Center for Tissue Restoration and Reconstruction, School of Materials Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong Province, China
  • About author:Meng Yong-chun, Wenzhou Engineering Research Center for Biomaterials, the Eye Hospital of Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China
  • Supported by:

    the Natural Science Foundation of Zhejiang Province, No. Y407241; the National Natural Science Foundation of China, No. 51072055, 50830101; the Major State Basic Research Development Program of China (973 Program), No. 2011CB606204

PDF

2147

被引次数

14

摘要：

背景：生物活性玻璃/胶原复合材料具有优良的成骨活性和的生物学性能，然而其在人体环境中易降解而导致支架溃散、力学性能下降。
目的：构建具有良好力学性能、抗降解性能和骨修复特性的胶原/生物活性玻璃/壳聚糖增强型复合支架。
方法：以壳聚糖作为分散剂，将生物活性玻璃粉体预先在壳聚糖溶液中均匀分散，然后与胶原溶液混合，结合冷冻干燥法制备多孔胶原/生物活性玻璃/壳聚糖增强型复合骨修复支架。采用傅里叶变换红外光谱仪、场发射扫描电子显微镜、X射线衍射仪、动态生物力学试验机等对复合支架的结构和性能进行表征。
结果与结论：由于壳聚糖和生物活性玻璃粉体在微酸性环境下的电荷吸引，使在壳聚糖中预分散的生物活性玻璃颗粒在复合支架中分散更均匀；壳聚糖的引入大量增加了机体中的羟基和氨基，使分子间的相互作用增强，显著提高了材料的抗压模量和强度；壳聚糖和胶原在分子尺度的混合，使胶原分子被壳聚糖包裹，降低了胶原酶对胶原分子的酶切能力，显著提高了复合支架的抗胶原酶解性；壳聚糖分子使生物活性玻璃颗粒更均匀的包裹在大分子基相中，减少了生物活性玻璃颗粒的团聚和暴露，导致复合支架在模拟体液中的矿化活性略微降低。


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 骨生物材料, 壳聚糖, 分散, 生物活性玻璃, 胶原, 支架, 国家自然科学基金

Abstract:

BACKGROUND: Collagen/bioactive glass composite materials possess excellent osteogenic potential and biocompatibility, but its application in bone tissue engineering is limited by mechanical property and degradation.
OBJECTIVE: To construct collagen/bioactive glass/chitosan composite scaffolds with good mechanical property, anti-degradation ability and bone repair property.
METHODS: Bioactive glass/collagen composite scaffolds with chitosan as dispersant were prepared by lyophylization. Fourier transform infrared spectroscopy, scanning electron microscope, X-ray diffraction, and dynamic biomechanical testing were used to characterize the structure and properties of the composite scaffolds.
RESULTS AND CONCLUSION: Results show that charge-attractions in pre-prepared bioactive glass/chitosan solution increased the homogeneity of bioactive glass dispersed in collagen gel and the compressive modulus and strength increased significantly due to the homogeneity and intermolecular interactions between chitosan and collagen. The enzymatic degradation rate and mineralization activity in the simulated body fluid were also lower because of a high degree of embedment of bioactive glass in collagen/chitosan matrix, and entanglement of collagen in chitosan at molecular level, which decreased the exposure of bioactive glass to the simulated body fluid, and collagen to enzyme solution.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: biocompatible materials, chitosan, collagen, glass

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