中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (46): 8556-8560.doi: 10.3969/j.issn.2095-4344.2012.46.002

• 骨组织构建 bone tissue construction • 上一篇    下一篇

雌激素干预吸烟骨质疏松大鼠种植体周围的骨沉积

侯玉东1，余萍萍2，韩晓鹏2，任光辉2，贾婷婷1   

  1. 1滨州医学院口腔医学院，山东省烟台市 264003
    2烟台市口腔医院口腔修复科，山东省烟台市 264001
  • 收稿日期:2012-03-02 修回日期:2012-04-18 出版日期:2012-11-11 发布日期:2013-03-16
  • 作者简介:侯玉东★，男，1965年生，山东省高唐县人，汉族，1997年湖北医科大学毕业，硕士，教授，主要从事口腔修复学的研究。 gongzuozaixian100@163.com

Effect of estrogen on the bone deposition around implants in osteoporosis rats undergoing cigarette smoke inhalation

Hou Yu-dong1, Yu Ping-ping2, Han Xiao-peng2, Ren Guang-hui2, Jia Ting-ting1   

  1. 1College of Stomatology, Binzhou Medical University, Binzhou 264003, Shandong Province, China
    2Department of Prosthodontics, Yantai Stomatological Hospital, Yantai 264001, Shandong Province, China
  • Received:2012-03-02 Revised:2012-04-18 Online:2012-11-11 Published:2013-03-16
  • About author:Hou Yu-dong★, Master, Professor, College of Stomatology, Binzhou Medical University, Binzhou 264003, Shandong Province, China gongzuozaixian100@163.com

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617

被引次数

5

摘要：

背景：骨质疏松、吸烟可致口腔种植失败率显著增高。
目的：观察雌激素对吸烟骨质疏松大鼠种植体周围骨沉积的影响。
方法：50只雌性大鼠均分为5组：除假手术组外，卵巢去势组、卵巢去势+雌激素组、卵巢去势+吸烟组、卵巢去势+吸烟+雌激素组均制备卵巢去势模型，去势术后后两组持续熏烟24周。去势术后12周，在大鼠右侧胫骨近骺端植入钛种植体，种植后对卵巢去势+雌激素组和卵巢去势+吸烟+雌激素组肌注雌激素。实验24周时行动物活体骨密度测量及X射线观察，处死动物采集标本行种植体骨硬组织切片观察。
结果与结论：骨密度及X射线观察结果显示，雌激素可提高种植体周围骨沉积。硬组织切片观察结果显示，卵巢去势+雌激素组种植体骨包绕范围基本形成骨结合，松质骨区结合骨板与周围骨小梁相连。卵巢去势+吸烟+雌激素组种植体周围结合骨板较卵巢去势+吸烟组明显增厚，骨板周围小梁骨增多。提示雌激素可在一定程度上提高吸烟骨质疏松大鼠种植体周围骨沉积，促进种植体骨结合。

关键词: 吸烟, 雌激素, 骨质疏松, 种植体, 骨结合, 大鼠, 组织构建

Abstract:

BACKGROUND: Osteoporosis and smoking can increase dental implant failure rate significantly.
OBJECTIVE: To investigate the effect of estrogen on bone deposition around implants in osteoporosis rats undergoing cigarette smoke inhalation.
METHODS: Fifty 3-month-old female SD rats were randomly divided into five groups (n=10): sham operation group, ovariectomy group, ovariectomy+smoke inhalation group, ovariectomy+estrogen group and ovariectomy+smoke inhalation+estrogen group. Rats in all groups were subjected to ovariectomy except the sham operation group. After ovariectomy, the latter two groups were given cigarette smoke inhalation for 24 weeks. Then, screw-type titanium implants were placed into the right tibiae in rats at 12 weeks after ovariectomy or sham surgery. Next, rats in the ovariectomy+estrogen group and ovariectomy+smoke inhalation+estrogen group were injected with estradiol benzoate intramuscularly. After 24 weeks, before sacrificed, densitometry of the tibiae in rats was detected by X-ray test. After that the samples of dead rats were collected and the tibiae with implant were removed by hand-mill piece of bone implant for observation.
RESULTS AND CONCLUSION: The densitometry and X-ray results demonstrated that estradiol could improve bone deposition around implants. Hard tissue slicing observations result showed that in ovariectomy+estrogen group, the implants obtained good osseointegration, besides, cancellous bone area combined with bone lamella was connected with the trabecular bone. In addition, the bonding bone lamella in ovariectomy+smoke inhalation+estrogen group was thicker than that in ovariectomy+smoke inhalation group. Moreover, the amount of trabecular bone was increased. These results suggest that estrogen can improve the bone deposition around implants to a certain extent and promote implant osseointegration in osteoporosis rats undergoing cigarette smoke inhalation.

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