中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (36): 6774-6778.doi: 10.3969/j.issn.2095-4344.2012.36.022

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

脊髓损伤大鼠内皮祖细胞与脊髓血管新生

魏正军1，朱 涛1，江荣才1，刘 丽2，刘 通1，孙石平1，余云湖1   

  1. 天津医科大学总医院，1神经外科，2神经病学研究所，天津市 300052
  • 收稿日期:2012-02-08 修回日期:2012-04-01 出版日期:2012-09-02 发布日期:2012-09-02
  • 通讯作者: 朱涛，博士，主任医师，教授，天津医科大学总医院神经外科，天津市300052 zhutao5@126.com
  • 作者简介:魏正军★，男，1986年生，山东省德州市人，天津医科大学在读硕士，主要从事脊髓创伤修复方面的研究。 weizhengjun0317@126.com

Correlation between endothelial progenitor cells and neovascularization following spinal cord injury in rats

Wei Zheng-jun1, Zhu Tao1, Jiang Rong-cai1, Liu Li2, Liu Tong1, Sun Shi-ping1, Yu Yun-hu1   

  1. 1Department of Neurosurgery, 2Institute of Neurology, General Hospital of Tianjin Medical University, Tianjin 300052, China
  • Received:2012-02-08 Revised:2012-04-01 Online:2012-09-02 Published:2012-09-02
  • Contact: Zhu Tao, M.D., Chief physician, Professor, Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin 300052, China zhutao5@126.com
  • About author:Wei Zheng-jun★, Studying for master’s degree, Department of Neurosurgery, General Hospital of Tianjin Medical University, Tianjin 300052, China weizhengjun0317@126.com

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394

被引次数

1

摘要：

背景：诸多脑损伤实验表明，改善损伤组织缺血缺氧有助于促进损伤组织的修复，并有保护神经功能的目的。
目的：探讨挫伤型脊髓损伤后大鼠外周血中内皮祖细胞的变化及其与创伤灶周围早期血管新生情况的关系。
方法：成年雄性SD大鼠60只，随机数字表法均分为实验组与对照组。实验组采用Allen’s原理制作脊髓损伤模型，对照组只行椎板切除暴露脊髓，不予打击致伤。分别于术前及术后3，6，24，48，72，168 h取大鼠外周血，流式细胞仪检测外周血中内皮祖细胞数量。并于术前及术后1，4，7，14 d处死大鼠取脊髓，采用血管内皮标志物CD31免疫组化染色方法观察创伤区血管的新生情况。
结果与结论：两组大鼠外周血内皮祖细胞水平于伤后3 h均有所下降，6 h开始上升，24 h达高峰，随后逐渐达稳定水平。实验组高峰值明显高于对照组。同时微血管数量呈逐渐增多的趋势。说明大鼠脊髓损伤后，外周血内皮祖细胞数量明显增加，内皮祖细胞参与血管新生和损伤组织的修复，最终促进神经功能恢复。

关键词: 脊髓损伤, 血管新生, 内皮祖细胞, 外周血, 大鼠

Abstract:

BACKGROUND: Many brain injury experiments have demonstrated that improving ischemia and hypoxia in injured tissue helps to promote the recovery of tissue injury and protect neurological function.
OBJECTIVE: To investigate the relationship of endothelial progenitor cells and early neovascularization following spinal cord injury in rats.
METHODS: A total of 60 Sprague-Dawley rats were divided into experimental groups ruined by modified Allen’s method and surgical controls that received laminectomy but did not receive a contusive injury. Peripheral venous blood samples were taken prior to surgery and at 3, 6, 24, 48, 72 and 168 hours after surgery, and the counts of endothelial progenitor cells were determined by flow cytometry. The rats were sacrificed on days 1, 4, 7 and 14. The morphological changes in neovascularization were observed, and the expression of CD31, a marker of vascular endothelial cell, was detected by immunohistochemistry.
RESULTS AND CONCLUSION: The levels of circulating endothelial progenitor cells within the first 3 hours of injury were lower than normal subjects, but increased over time, and reached a peak around 24 hours post-injury at a level that was significantly higher than controls. The change in circulating endothelial progenitor cells was obviously correlated with the change of CD31+ vessels in the region adjacent to the epicenter of the injury site. The results demonstrate a close correlation between an increase in circulating endothelial progenitor cells in response to spinal cord injury and angiogenesis in spinal cord injury rat spinal cord. They also suggest that the increase in circulating endothelial progenitor cells promotes the tissue recovery in spinal cord injury.

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