中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (27): 4959-4962.doi: 10.3969/j.issn. 2095-4344.2012.27. 004

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

兔骨髓间充质干细胞与羊膜的共培养

张治金，郭 林，赵德伟   

  1. 大连大学附属中山医院骨科，辽宁省大连市 116001
  • 收稿日期:2011-12-03 修回日期:2012-01-17 出版日期:2012-07-01 发布日期:2013-11-01
  • 通讯作者: 郭林，硕士，教授，大连大学附属中山医院骨科，辽宁省大连市 116001 glds2010@ 163.com
  • 作者简介:张治金★，男，1982年生，安徽省临泉县人，汉族，大连大学在读硕士，主要从事关节外科研究。 zzj20030606@ qq.com

Co-culture of rabbit bone marrow mesenchymal stem cells and human amniotic membrane

Zhang Zhi-jin, Guo Lin, Zhao De-wei   

  1. Department of Orthopedics, Zhongshan Hospital Affiliated to Dalian Medical University, Dalian 116001, Liaoning Province, China
  • Received:2011-12-03 Revised:2012-01-17 Online:2012-07-01 Published:2013-11-01
  • Contact: Guo Lin, Master, Professor, Department of Orthopedics, Zhongshan Hospital Affiliated to Dalian Medical University, Dalian 116001, Liaoning Province, China glds2010@163.com
  • About author:Zhang Zhi-jin, Studying for master’s degree, Department of Orthopedics, Zhongshan Hospital Affiliated to Dalian Medical University, Dalian 116001, Liaoning Province, China zzj20030606@ qq.com

PDF

357

被引次数

4

摘要：

背景：近年来利用组织工程技术构建软骨以修复软骨缺损已经取得较大进步。骨髓间充质干细胞在软骨组织工程研究中作为一种常见的种子细胞被广泛应用。
目的：以人羊膜作为细胞支架负载兔骨髓间充质干细胞，观察羊膜的生物相容性。
方法：将兔骨髓间充质干细胞接种于人脱细胞羊膜上，体外共培养后进行形态学观察，以四甲基偶氮唑盐比色法观察骨髓间充质干细胞增殖活性，评价羊膜的组织相容性。应用4’，6-二脒基-2-苯基吲哚染色法观察细胞存活和凋亡情况。
结果与结论：骨髓间充质干细胞在羊膜上生长较好，细胞活力增强，4’，6-二脒基-2-苯基吲哚染色结果示仅有少量细胞凋亡。提示羊膜是一种具有良好生物相容性的支架材料，可以促进骨髓间充质干细胞增殖。

关键词: 羊膜, 骨髓间充质干细胞, 生物相容性, 兔, 细胞支架, 干细胞

Abstract:

BACKGROUND: In recent years, great progress has been made in repair of cartilage defects using tissue engineering technique. Bone marrow mesenchymal stem cells (BMSCs) as common seed cells have been widely used in the tissue engineering of cartilage tissue.
OBJECTIVE: Human amniotic membrane was used as cell scaffold to load rabbit BMSCs and the biocompatibility of human amniotic membrane was observed.
METHODS: Rabbit BMSCs were inoculated onto human acellular amnion. After co-culture, cell morphology was observed. The proliferation of BMSCs was investigated by MTT assay, and the histocompatibility of human amniotic membrane was evaluated. Cell survival and apoptosis was determined by 4’, 6-diamidino-2-phenylindole.
RESULTS AND CONCLUTION: BMSCs grew well on the surface of human amniotic membrane and cell viability increased. DAPI staining results showed that only a small number of cells were apoptotic. These findings suggest that human amniotic membrane is a scaffold material with good biocompatibility and can promote the proliferation of BMSCs.

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