中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (25): 4596-4600.doi: 10.3969/j.issn.1673-8225.2012.25.008

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米氧化锌对白色念珠菌的抗菌作用

何临海1，孟 松1，柳苗苗1，周迪舜1，李 珊1，罗 梅2，马兴铭3，王雪梅1   

  1. 兰州大学，1口腔医学院，3基础医学院，甘肃省兰州市 730000；2解放军兰州军区总医院安宁分院口腔科，甘肃省兰州市 730000
  • 收稿日期:2011-12-19 修回日期:2012-01-05 出版日期:2012-06-17 发布日期:2013-11-04
  • 通讯作者: 王雪梅，讲师，兰州大学口腔医学院，甘肃省兰州市 730000 wangxuemei@ lzu.edu.cn
  • 作者简介:何临海，男，1988年生，河南省开封市人，兰州大学口腔医学院在读本科。 Heinhai07@ 126.com
  • 基金资助:

    兰州大学口腔医学扶持基金(533000)；甘肃省自然科学基金(1010RJ2A112)；教育部2010质量工程--大学生科技创新实验计划(101073038)

Antibacterial effects of nano zinc oxide on Candida albicans

He Lin-hai1, Meng Song1, Liu Miao-miao1, Zhou Di-shun1, Li Shan1, Luo Mei2, Ma Xing-ming3, Wang Xue-mei1   

  1. 1School of Stomatology, 3School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, Gansu Province, China;
    2Department of Stomatology, Anning Branch, General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730000, Gansu Province, China
  • Received:2011-12-19 Revised:2012-01-05 Online:2012-06-17 Published:2013-11-04
  • Contact: Wang Xue-mei, Lecturer, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China wangxuemei@ lzu.edu.cn
  • About author:He Lin-hai, School of Stomatology, Lanzhou University, Lanzhou 730000, Gansu Province, China Heinhai07@126.com

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1771

被引次数

18

摘要：

背景：纳米氧化锌作为无机抗菌剂，与有机杀菌剂的抗菌机制明显不同。
目的：探讨纳米氧化锌对白色念珠菌等真菌的抗菌作用。
方法：分别配制成100，75，50，25，10 g/L的纳米氧化锌与普通氧化锌悬浊液，以生理盐水作为阴性对照，采用KB纸片扩散法将30 μL不同浓度悬浊液分别作用于白色念珠菌，将培养平板上置于37 ℃恒温箱培养24 h观察并测量抑菌圈大小。
结果与讨论：随着纳米氧化锌浓度的提高，对白色念珠菌的抑制作用逐渐增强，抑菌圈直径逐渐增大。普通氧化锌的无明显抑菌作用。证实纳米氧化锌能够抑制白色念珠菌等真菌的生长。

关键词: 纳米氧化锌, 白色念珠菌, 真菌, 抑菌圈, 抑菌作用, 生物材料

Abstract:

BACKGROUND: As an inorganic antibacterial agent, nano zinc oxide has different antibacterial mechanisms from organic fungicides.
OBJECTIVE: To explore the antibacterial effects of nano zinc oxide on the fungus, such as Candida albicans.
METHODS: Nano zinc oxide and zinc oxide were prepared as the suspension with concentrations of 100, 75, 50, 25 and 10 g/L respectively. Normal saline served as a negative control. 30 μL suspension with different concentrations was acted on Candida albicans by using Kirby-Bauer disk diffusion method. The culture plate was cultured at a 37 ℃ incubator for 24 hours. The size of inhibition zone was observed and measured.
RESULTS AND CONCLUSION: With the increased concentration of nano zinc oxide, the antibacterial effect on Candida albicans was enhanced gradually, and the diameter of inhibition zone was enhanced. There were no obvious antibacterial effects of zinc oxide on Candida albicans. These findings suggest that nano zinc oxide can inhibit the growth of Candida albicans.

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