中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (19): 3433-3437.doi: 10.3969/j.issn.1673-8225.2012.19.003

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

雌激素剂量依赖性促进小鼠骨髓间充质干细胞的成骨分化★

朱晓斐1，王  飏2，金  岩3，贾立辉4   

  1. 1辽宁医学院解放军沈阳军区总医院研究生培养基地，辽宁省沈阳市  110016；解放军沈阳军区总医院，2整形外科，4科训科，辽宁省沈阳市  110016；3解放军第四军医大学口腔医学院组织工程中心，陕西省西安市  710032
  • 收稿日期:2012-03-01 修回日期:2012-03-19 出版日期:2012-05-06 发布日期:2012-05-06
  • 通讯作者: 贾立辉，硕士生导师，副主任医师，解放军沈阳军区总医院科训科，辽宁省沈阳市 110016
  • 作者简介:朱晓斐★，女，1986年生，山东省烟台市人，汉族，辽宁医学院在读硕士，主要从事口腔临床医学颌面外科研究。xiaofei.987@163.com

Estrogen promotes osteogenic differentiation of mouse bone marrow mesenchymal stem cells in a dose-dependent manner  

Zhu Xiao-fei1, Wang Yang2, Jin Yan3, Jia Li-hui4   

  1. 1Postgraduate Culture Base, the General Hospital of Shenyang Military Region, Liaoning Medical University, Shenyang  110016, Liaoning Province, China; 2Department of Plastic Surgery, 4Department of Training, the General Hospital of Shenyang Military Region, Shenyang  110016, Liaoning Province, China; 3Tissue Engineering Center, School of Stomatology, Fourth Military Medical University of Chinese PLA, Xi’an   710032, Shaanxi Province, China
  • Received:2012-03-01 Revised:2012-03-19 Online:2012-05-06 Published:2012-05-06
  • Contact: Jia Li-hui, Master’s supervisor, Associate chief physician, Department of Training, the General Hospital of Shenyang Military Region, Shenyang 110016, Liaoning Province, China
  • About author:Zhu Xiao-fei★, Studying for master’s degree, Postgraduate Culture Base, the General Hospital of Shenyang Military Region, Liaoning Medical University, Shenyang 110016, Liaoning Province, China

PDF

477

被引次数

8

摘要：

背景：绝经后骨质疏松的发病与雌激素水平的下降关系密切。
目的：观察不同浓度雌激素对小鼠骨髓间充质干细胞成骨分化能力的影响，及其与微小RNA-26a的关系。
方法：取小鼠股骨与胫骨骨髓，全骨髓贴壁法获得并纯化骨髓间充质干细胞，分别以0，10-10，10-9，10-8，10-7，10-6 mol/L的雌二醇对其成骨诱导过程进行干预。
结果与结论：雌二醇对骨髓间充质干细胞的增殖能力影响不明显，但可明显提高其成骨能力；同时雌二醇可促进骨髓间充质干细胞成骨基因RUNX2，OCN mRNA及RUNX2，SP7蛋白的表达，以10-9 mol/L雌二醇的作用最明显，但10-9 mol/L雌二醇促进微小RNA-26a mRNA表达的能力最弱。说明雌二醇可剂量依赖性促进骨髓间充质干细胞的成骨分化，微小RNA-26a可能在此过程中发挥作用。
关键词：微小RNA-26a；雌激素；骨髓间充质干细胞；成骨分化；小鼠
doi:10.3969/j.issn.1673-8225.2012.19.003

关键词: 微小RNA-26a, 雌激素, 骨髓间充质干细胞, 成骨分化, 小鼠

Abstract:

BACKGROUND: The pathogenesis of postmenopausal osteoporosis and estrogen deficiency is closely related.
OBJECTIVE: To investigate the effects of different concentrations of estrogen on osteogenic differentiation of mouse bone marrow mesenchymal stem cells (BMSCs) and the relationship with miRNA-26a.
METHODS: Mouse femoral and tibial bone marrow was taken and BMSCs were isolated and purified by the whole bone marrow adherence method. Estrogen at different concentrations (0, 10-10, 10-9, 10-8, 10-7, 10-6 mol/L) was used for osteogenic induction.
RESULTS AND CONCLUSION: Estrogen did not greatly influence the proliferation of BMSCs, but it could significantly enhance the osteogenic differentiation capability of BMSCs. At the same time, estrogen, in particular at a concentration of 10-9 mol/L, could promote RUNX2, OCN mRNA and RUNCX2, SP7 protein expression in BMSCs. However, 10-9 mol/L estrogen promoted miRNA-26a mRNA expression at the weakest capability. These findings suggest that estrogen can promote osteogenic differentiation of BMSCs in a dose-dependent manner, and microRNA-26a possibly exerts effects during this process.

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