中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (16): 2943-2946.doi: 10.3969/j.issn.1673-8225.2012.16.022

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

4种烤瓷冠基底合金浸提液影响人牙龈成纤维细胞中Bcl-2和Bax的表达★

刘  婷，黄克强，李志刚，安英杰   

  1. 辽宁医学院附属口腔医院，辽宁省锦州市  121004
  • 收稿日期:2011-12-27 修回日期:2012-02-29 出版日期:2012-04-15 发布日期:2012-04-15
  • 通讯作者: 黄克强，硕士生导师，教授，辽宁医学院附属口腔医院，辽宁省锦州市 121004 hkq9@163.com
  • 作者简介:刘婷★，女，1983年生；辽宁省辽阳市人，汉族，2011年辽宁医学院毕业，硕士，医师，主要从事烤瓷冠基底合金对牙周组织影响的研究。liouting_1018@ yahoo.com.cn

Effects of four species of metal alloys on the expression of Bcl-2 and Bax in human gingival fibroblast cells

Liu Ting, Huang Ke-qiang, Li Zhi-gang, An Ying-jie   

  1. Stomatology Hospital of Liaoning Medical University, Jinzhou  121004, Liaoning Province, China
  • Received:2011-12-27 Revised:2012-02-29 Online:2012-04-15 Published:2012-04-15
  • Contact: author: Huang Ke-qiang, Master’s supervisor, Professor, Stomatology Hospital of Liaoning Medical University, Jinzhou 121004, Liaoning Province, China hkq9@163.com
  • About author:Liu Ting★, Master, Physician, Stomatology Hospital of Liaoning Medical University, Jinzhou 121004, Liaoning Province, China liouting_1018@yahoo.com.cn

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摘要：

背景：研究显示，纯钛材料对人牙龈成纤维细胞的影响不明显，而镍铬合金、含钛镍铬合金明显影响了细胞的炎性表达。
目的：比较4种烤瓷冠基底合金对人牙龈成纤维细胞中Bcl-2和Bax表达的影响。
方法：用组织块法分离培养人牙龈成纤维细胞，实验组分别用4种烤瓷冠基底合金浸提液加入人牙龈成纤维细胞培养液中，空白对照组用含体积分数10%小牛血清的DMEM培养液，Western-blot法检测人牙龈成纤维细胞中Bcl-2和Bax表达的改变。
结果与结论：金合金组对Bcl-2和Bax表达的影响接近空白对照组，纯钛组和钴铬合金组对Bcl-2和Bax表达的影响较小，镍铬合金组对Bcl-2和Bax表达的影响最大，与其他各组相比差异有显著性意义(P < 0.05)。提示镍铬合金可通过影响Bcl-2与Bax的表达水平，抑制人牙龈成纤维细胞的增殖，促进细胞凋亡。
关键词：烤瓷冠基底合金；人牙龈成纤维细胞；Bcl-2；Bax；钛
缩略语注释：HGFs：human gingival fibroblasts，人牙龈成纤维细胞
doi:10.3969/j.issn.1673-8225.2012.16.022

关键词: 烤瓷冠基底合金, 人牙龈成纤维细胞, Bcl-2, Bax, 钛

Abstract:

BACKGROUND: Studies have shown there are no significant effects of titanium materials on human gingival fibroblasts. However, nickel-chromium alloy, titanium-nickel-chromium alloy significantly affect the inflammatory expression of the cells.
OBJECTIVE: To investigate the effects of four species of metal alloys on the expression of Bcl-2 and Bax in human gingival fibroblast cells.
METHODS: Human gingival fibroblasts were isolated and cultured using tissue block method. Four kinds of metal alloy extracts were used and added into culture medium of human gingival fibroblast cells, and the blank control group was treated with Dulbecco's modified Eagle’s medium containing 10% calf serum. Western-blot assay was used to detect the expression of Bcl-2 and Bax in human gingival fibroblast cells.
RESULTS AND CONCLUSION: The Bcl-2 and Bax expression of the gold alloy group was closed to that of the blank control group. The Bcl-2 and Bax expression changed insignificantly in the pure titanium and cobalt chromium alloy groups, but had a great change in the nickel-chromium alloy group (P < 0.05). Nickel-chromium alloy can affect the Bcl-2 and Bax expression levels, inhibit human gingival fibroblast proliferation, and promote human gingival fibroblast apoptosis.

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