中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (1): 35-38.doi: 10.3969/j.issn.1673-8225.2012.01.007

• 胚胎干细胞 embryonic stem cells • 上一篇    下一篇

胎源性非黏附骨髓基质细胞的特性*★ 胎源性非黏附骨髓基质细胞的特性*★

肖龙艳1，傅晋翔1，张学光2，陈  秋2，张  宏1，王盼君1，孙  谕1，王明元3，古彦铮2   

  1. 1苏州大学附属第二医院，江苏省苏州市  215004；  2苏州大学生物技术研究所，江苏省苏州市 215000；3苏州市中心血站，江苏省苏州市  215000
  • 收稿日期:2011-08-15 修回日期:2011-11-24 出版日期:2012-01-01 发布日期:2012-01-01
  • 通讯作者: 傅晋翔，教授，苏州大学附属第二医院，江苏省苏州市 215004 uufjxly@public1.sz.js.cn
  • 作者简介:Supported by: 肖龙艳★，女，1984年生，山东省临沂市人，汉族，苏州大学在读硕士，主要从事血液免疫方面的研究。 Xiaolongyan1984@sina.com
  • 基金资助:

    国家自然科学基金资助项目(81071943)。
    作者贡献：通讯作者进行实验设计，实验实施和实验评估为全部作者，资料收集为第一作者，第一作者成文，通讯作者审校，所有作者对文章负责。

Characteristics of fetal non-adherent bone marrow stromal cells 

Xiao Long-yan1, Fu Jin-xiang1, Zhang Xue-guang2, Chen Qiu2, Zhang Hong1, Wang Pan-jun1, Sun Yu1, Wang Ming-yuan3,
Gu Yan-zheng2
 
  

  1. 1Second Affiliated Hospital of Soochow University, Suzhou  215004, Jiangsu Province, China; 2Institute of Biotechnology of Soochow University, Suzhou  215007, Jiangsu Province, China; 3Suzhou Red Cross Blood Centre, Suzhou  215006, Jiangsu Province, China
  • Received:2011-08-15 Revised:2011-11-24 Online:2012-01-01 Published:2012-01-01
  • Contact: Fu Jin-xiang, Professor, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China uufjxly@public1.sz.js.cn
  • About author:Xiao Long-yan★, Studying for master’s degree, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China Xiaolongyan1984@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81071943*

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摘要：

 

背景：骨髓中存在非黏附干细胞，该类细胞形态及表型特征与黏附骨髓间充质干细胞相似。
目的：观察胎源性非黏附骨髓基质细胞的基本特性。
方法：采用反复贴壁分离法培养胎源性骨髓非黏附基质细胞，每24 h取悬浮细胞分瓶培养，共4次；采用MTT法测定第1，3，5代细胞生长曲线，流式细胞仪行细胞周期测定和表面标志分析；并取第1代细胞行造血集落培养，第3代细胞成骨和成脂诱导培养。
结果与结论：反复贴壁分离培养4次后仍有细胞贴壁并有集落形成，剩余细胞经培养7 d后即可得到原代骨髓非黏附基质细胞。骨髓非黏附基质细胞形态不均匀，高表达整合素及内皮细胞表面标记，中等量表达CD106，同时也表达造血细胞标记CD34；体外可分化为成骨细胞及造血细胞，但成脂分化能力较弱。

关键词:

Abstract:

Abstract
BACKGROUND: There are non-adhesion stem cells in bone marrow stem cells, and the morphology and phenotype characteristics of the cells are similar to adhesion bone marrow mesenchymal stem cells.  
OBJECTIVE: To explore the characteristics of fetal non-adherent bone marrow stromal cells (NA-BMSCs).
METHODS: The fetal NA-BMSCs were cultivated by the method of adherent separation repeatly (“pull-offs”), the suspended cells were incubated in another culture bottle every 24 hours for 4 times. The growth curve of the passage 1, 3, 5 NA-BMSCs was detected by MTT assay, and the phenotype of the NA-BMSCs was determined with flow cytometry. Passage 1 NA-BMSCs were preformed with hematopoietic colony assay and passage 3 NA-BMSCs were preformed with osteogenic and adipogenic induction.
RESULTS AND CONCLUSION: After four times’ “pull-off”, there were still asherent cells and colony formation; the resulting cells were continuing to culture for 7 days and those cells were the primary NA-MSCs (P0). The morphology of the cells was uneven; they highly expressed the integrin and the surface markers of endothelial cells with a middle level of CD106. Interestingly, they also expressed the surface markers of hematopoietic cells CD34. Those cells had multi-differentiation potency in vitro and they could differentiate into osteoblasts and hematopoietic cells under different inducing medium, but the capability of lipid differentiation was weak.

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