中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (7): 1195-1198.doi: 10.3969/j.issn.1673-8225.2011.07.012

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

转化生长因子β1与体外血管外膜成纤维细胞的增殖和迁移

马绍骏，蔡文玮，盛  净   

  1. 上海交通大学医学院附属第九人民医院老年病科 200011
  • 收稿日期:2010-08-19 修回日期:2010-09-05 出版日期:2011-02-12 发布日期:2011-02-12
  • 通讯作者: 盛净，硕士，主任医师，主要从事心血管方面的研究。上海交通大学医学院附属第九人民医院老年病科 200011 shengjing60@ 163.com
  • 作者简介:马绍骏★，女，1974年生，安徽省肥东县人，汉族，1997年上海第二医科大学毕业，硕士，主治医师，主要从事老年病学方面的研究。 mashj@163.com
  • 基金资助:

    上海交通大学医学院科技基金资助(06XJ21002)。

Transforming growth factor beta 1 on proliferation and migration of adventitia fibroblasts in vitro

Ma Shao-jun, Cai Wen-wei, Sheng Jing   

  1. Department of Geriatrics, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai  200011, China
  • Received:2010-08-19 Revised:2010-09-05 Online:2011-02-12 Published:2011-02-12
  • Contact: Sheng Jing, Master, Chief physician, Department of Geriatrics, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China shengjing60@163. com
  • About author:Ma Shao-jun★, Master, Attending physician, Department of Geriatrics, Shanghai Ninth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 200011, China mashj@163.com
  • Supported by:

    the Science and Technology Foundation of Shanghai Jiao Tong University School of Medicine, No. 06XJ21002*

PDF

438

摘要：

背景：近年来的研究表明，血管外膜成纤维细胞在血管损伤后新生内膜的增生中起重要作用。当血管损伤后，转化生长因子β1在局部水平上调激活下游多种信号途径，其对血管外膜成纤维细胞参与新生内膜增生过程中所起的作用尚不十分清楚。
目的：观察转化生长因子β1对体外血管外膜成纤维细胞增殖和迁移的影响
方法：体外培养大鼠胸主动脉成纤维细胞，分别以0，3，5，10，15 μg/L的转化生长因子β1刺激成纤维细胞0，2，12，24，48，72 h。以MTT法检测细胞增殖活性，Transwell Chamber测试细胞迁移。
结果与结论：转化生长因子β1能诱导血管外膜成纤维细胞增殖，随着转化生长因子β1质量浓度、作用时间的增加，诱导血管外膜成纤维细胞增殖能力增强(P< 0.05)，其中10 μg/L转化生长因子β1作用48 h增殖较对照组增强最为明显(P < 0.01)。不同剂量转化生长因子β1刺激后迁移的细胞数目随转化生长因子β1质量浓度增加而增加，促进作用逐渐增强，呈剂量依赖关系。

关键词: 转化生长因子&beta, 1, 血管外膜成纤维细胞, 细胞增殖, 细胞迁移, 血管重塑

Abstract:

BACKGROUND: Previous studies demonstrated that adventitia fibroblasts exhibit important role in the hyperplasia of newly born endomembrane after blood vessel injury. Transforming growth factor β1 (TGF-β1) can stimulate many signways after blood vessel injury. However, the effect of TGF-β1 on proliferation and migration of adventitia fibroblasts in vitro remains unclear.
OBJECTIVE: To observe the effect of TGF-β1 on proliferation and migration of adventitia fibroblasts in vitro.
METHODS: Fibroblasts from rat thoracic aorta were in vitro cultured and stimulated by TGF-β1 with different concentrations (0, 3, 5, 10 and 15 μg/L) for different hours (0, 2, 12, 24, 48, and 72 hours). MTT assay were applied to assess cellular proliferation. Migration assay was performed by transwell chambers.
RESULTS AND CONCLUSION: TGF-β1 could potentiate the proliferation of adventitia fibroblasts, and the effects were increased with the increasing of TGF-β1 concentrations and TGF-β1 inducing times (P < 0.05), especially stronger in the group treated by 10 μg/L TGF-β1 for 48 hours (P < 0.01). The migration of adventitia fibroblasts were increased with the increasing of TGF-β1 concentrations in a dose-dependent fashion.

中图分类号: