中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (53): 9934-9937.doi: 10.3969/j.issn.1673-8225.2011.53.014

• 肾移植 kidney transplantation • 上一篇    下一篇

口服供体脾细胞对大鼠移植肾的影响

余雪松1，黄赤兵1，邹升安2，范明齐1，王平贤1，冯嘉瑜1，肖  亚1   

  1. 1解放军第三军医大学附属新桥医院泌尿外科，重庆市 400037
    2解放军77118部队，四川省什邡市 614819
  • 收稿日期:2011-09-23 修回日期:2011-10-08 出版日期:2011-12-31 发布日期:2011-12-31
  • 通讯作者: 黄赤兵，博士，教授，主任医师，解放军第三军医大学附属新桥医院泌尿外科，重庆市 400037 huangchibing@medmail.com.cn
  • 作者简介:余雪松☆，男，1979年生，湖北省鄂州市人，汉族，2003年于解放军第三军医大学毕业，博士，主要从事移植免疫研究。 yxs197994@163.com
  • 基金资助:

    国家自然基金资助项目(30872580)，口服MHC肽诱导的受体Treg源性exosome干预肾移植排斥反应的机制研究。

Effects of oral administration of donor spleen cells on kidney transplantation in rats

Yu Xue-song1, Huang Chi-bing1, Zou Sheng-an2, Fan Ming-qi1, Wang Ping-xian1, Feng Jia-yu1, Xiao Ya1   

  1. 1Department of Urology, Xinqiao Hospital, Third Military Medical University of Chinese PLA, Chongqing 400037, China
    2Chinese PLA No.77118 Troop, Shifang  614819, Sichuan Province, China
  • Received:2011-09-23 Revised:2011-10-08 Online:2011-12-31 Published:2011-12-31
  • Contact: Huang Chi-bing, Doctor, Professor, Chief physician, Department of Urology, Xinqiao Hospital, Third Military Medical University of Chinese PLA, Chongqing 400037, China huangchibing@medmail.com.cn
  • About author:Yu Xue-song☆, Doctor, Department of Urology, Xinqiao Hospital, Third Military Medical University of Chinese PLA, Chongqing 400037, China yxs197994@163.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30872580*

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摘要：

背景：口服供体抗原诱导免疫耐受已被证实有显著效果，而脾脏为人体最大淋巴器官，富含T淋巴细胞，可提供丰富抗原。
目的：观察口服供体脾细胞对大鼠肾移植移植肾功能影响。
方法：肾移植前脾细胞组Lewis (RT11)大鼠采取口服灌胃5×105个BN (RT1n)大鼠供体脾细胞，1次/d，持续7 d；肾移植组Lewis (RT11)大鼠口服灌胃1 mL PBS为对照。
结果与结论：移植后第5天肾移植组出现移植肾排斥症状，脾细胞组平均存活时间长于肾移植组，移植后脾细胞组血肌酐、尿素氮水平升高明显慢于肾移植组；苏木精-伊红染色显示肾移植组移植肾发生急性排斥变化早于脾细胞组。说明口服供体脾细胞能诱导免疫耐受，延长移植肾存活时间。

关键词: 口服耐受, 肾移植, 移植物排斥, 抗原, 脾细胞, 器官移植

Abstract:

BACKGROUND: It is proved that oral administration of donor antigen can induce immunology tolerance effectively. The spleen is the biggest human lymphoid organ containing many T lymphocytes and can provide abound antigens.
OBJECTIVE: To observe the effects of oral administration of donor spleen cells on rat kidney function after transplantation.
METHODS: Before kidney transplantation, Lewis (RT11) rats in splenocytes group were fed with 5×105 donor splenocytes by gastric intubation daily for 7 days, while Lewis (RT11) rats in transplantation group were fed with 1 mL PBS as control.
RESULTS AND CONCLUSION: On the 5th day after transplantation, allografts rejection symptoms began appearing in the transplantation group. Mean survival time of splenocytes group was longer than that of the transplantation group. After transplantation, the serum creatinine and urea nitrogen level in splenocytes group increased slower than that in the transplantation group. According to hematoxylin-eosin staining, the acute rejection occurred earlier in transplantation group than that in the splenocytes group. It demonstrates that oral administration of donor spleen cells can induce immune tolerance and prolong the survival time of kidney allografts.

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