中国组织工程研究

中国组织工程研究 ›› 2011, Vol. 15 ›› Issue (31): 5822-5826.doi: 10.3969/j.issn.1673-8225.2011.31.029

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

大部分肝切除模型大鼠肝再生组织中CD34蛋白及Delta-like-1蛋白的表达

杨志云1，侯艺鑫2，王宪波1   

  1. 1首都医科大学附属北京地坛医院中西医结合中心，北京市  100015
    2北京中医药大学，北京市  100029
  • 收稿日期:2011-05-06 修回日期:2011-06-19 出版日期:2011-07-30 发布日期:2011-07-30
  • 通讯作者: 杨志云，副主任医师，硕士生导师，首都医科大学附属北京地坛医院中西医结合中心，北京市 100015 并列通讯作者：王宪波，主任医师，博士生导师，首都医科大学附属北京地坛医院中西医结合中心，北京市 100015
  • 作者简介:杨志云☆，女，1972年生，河北省石家庄市人，汉族，2007年河北医科大学毕业，博士，副主任医师，主要从事中医药诱导肝干细胞分化基础与临床研究。
  • 基金资助:

    国家自然科学基金项目(30873423)；北京市中医管理局青年科学研究资助项目(QN2010-7)。

Expressions of CD34，Delta-like-1 protein in hepatic regeneration tissue of rats after partial hepatectomy

Yang Zhi-yun1, Hou Yi-xin2, Wang Xian-bo1　   

  1. 1Department of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing  100015, China
    2Beijing University of Chinese Medicine, Beijing  100029, China
  • Received:2011-05-06 Revised:2011-06-19 Online:2011-07-30 Published:2011-07-30
  • Contact: Yang Zhi-yun, Doctor, Associate chief physician, Master’s supervisor, Department of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China yangzhiyun6@ yahoo.com.cn Correspondence to: Wang Xian-bo, Chief physician, Doctoral supervisor, Department of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China wangxianbo638@ 163.com
  • About author:Yang Zhi-yun☆, Doctor, Associate chief physician, Master’s supervisor, Department of Integrated Traditional Chinese and Western Medicine, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China
  • Supported by:

    the National Natural Science Foundation of China, No. 30873423*; the Youth Science Research Program of Administration of Traditional Chinese Medicine of Beijing City, No. QN2010-7*

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摘要：

背景：当肝脏受到严重损伤或肝脏大部分缺失时，肝干细胞迅速分裂增殖向成熟肝细胞分化修复肝组织，但肝再生是一个复杂的多信号分子调控的过程，参与调控的相关基因及蛋白质目前尚不十分清楚。
目的：观察大部分肝切除后大鼠肝再生组织中CD34蛋白、Delta-like-1蛋白的表达变化。
方法：切除2/3肝脏建立大鼠肝再生动物模型，应用苏木精-伊红染色、酶组织化学染色、免疫组织化学染色方法在不同时间点检测肝再生组织中ATP酶、增殖细胞核抗原、CD34、Delta-like-1蛋白的表达情况。
结果与结论：在肝再生的启动阶段ATP酶表达下调，CD34、Delta-like-1表达上调；在肝再生的持续增殖阶段，ATP酶表达上调到正常水平，CD34、Delta-like-1表达下调；增殖细胞核抗原表达为持续上调。说明ATP酶、CD34、Delta-like-1在不同阶段表达不同，提示在肝再生过程中各信号分子的协同作用及Notch- Delta信号通路参与其作用机制。

关键词: 肝再生, CD34, Delta-like-1, ATP酶, 增殖细胞核抗原

Abstract:

BACKGROUND: When the liver affected severe damage or excalation, liver stem cells rapidly proliferated and differentiated into mature liver cells to repair liver tissue. However, liver regeneration is a complicated multiple signal molecular regulatory process and related genes and proteins that participated in regulation remain unclear.
OBJECTIVE: To investigate the expressions of CD34 and Delta-like-1 protein in rat liver regenerated tissues after partial hepatectomy.
METHODS: Rat hepatic regeneration models were established after hepatectomy. Expressions of ATPase, proliferating cell nuclear antigen (PCNA), CD34 and Delta-like-1 protein were detected by hematoxylin-eosin staining, enzyme-histochemistry and immunohistochemistry method at various time points.
RESULTS AND CONCLUSION: At the early stage of liver regeneration, the expression of ATPase was down-regulated, and the expressions of CD34 and Delta-like-1 protein were up-regulated. At the middle stage, the expression of ATPase was increased to the normal level, and the expressions of CD34 and Delta-like-1 protein were down-regulated. The expression of PCNA protein was continuously up-regulated. These suggest that the expressions of ATPase, CD34 and Delta-like-1 protein were different at different stages of hepatic regeneration, which means that different signal molecules were cooperated during the hepatic regeneration, and Notch-Delta signal pathway takes part in the pathogenesy.

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