中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (4): 669-672.doi: :10.3969/j.issn.1673-8225.2010.04.023

• 骨与关节损伤基础实验 basic experiments of bone and joint injury • 上一篇    下一篇

金属离子对单核/巨噬细胞细胞活性及其膜上RANK表达的影响

戴  闽，陈  锐，詹  平，袁晓军，艾江波，程  明   

  1. 南昌大学第一附属医院骨二科，江西省南昌市 330006
  • 出版日期:2010-01-22 发布日期:2010-01-22
  • 作者简介:戴 闽★，男，1963年生，江西省安义县人，汉族，1993年原上海第二医科大学毕业，硕士，主任医师，主要从事人工关节基础与临床研究。 achenrui_0@chinaren.com
  • 基金资助:

    课题受国家自然科学基金资助(30760255)。

Effect of metal ions on monocyte-macrophage cells viability and RANK expression

Dai Min, Chen Rui, Zhan Ping, Yuan Xiao-jun, Ai Jiang-bo, Cheng Ming   

  1. Second Department of Orthopaedics, the First Affiliated Hospital of Nanchang University, Nanchang  330006, Jiangxi Province, China
  • Online:2010-01-22 Published:2010-01-22
  • About author:Dai Min★, Master, Chief physician, Second Department of Orthopaedics, the First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China achenrui_0@chinaren.com
  • Supported by:

     the National Natural Science Foundation of China, No. 30760255*

PDF

609

被引次数

11

摘要：

背景：与其他配伍的假体一样，金属-金属假体在使用过程中也会产生大量的磨损颗粒和金属离子，其中金属离子以钴和铬离子较为常见，可导致假体周围骨溶解，引起假体无菌性松动。
目的：观察Co 2+ 、Cr 3+离子对体外培养小鼠单核/巨噬细胞(RAW264.7)的细胞活性及其膜上RANK表达的影响。
方法：体外培养单核/巨噬细胞(RAW264.7)，采用金属钴铬离子对单核/巨噬细胞进行干预，不同时间点用四唑盐比色方法检测细胞活性并以半定量反转录-聚合酶链反应方法测定RANK mRNA的表达量。
结果与结论：四唑盐比色实验结果显示，与对照组相比，Co 2+ 、Cr 3+可使单核/巨噬细胞的细胞活性明显下降。单核/巨噬细胞暴露在钴铬离子下，与对照组相比，钴铬离子组单核/巨噬细胞RANKmRNA在12 h表达增强(P < 0.05)，24 h达到高峰(P < 0.05)，48 h较24 h表达下降(P < 0.05)。结果提示，金属离子对单核/巨噬细胞有细胞毒性，且能够刺激单核/巨噬细胞RANK mRNA的表达，为单核/巨噬细胞向具有骨质吸收功能的破骨样细胞转化提供必要的前提条件。

关键词: 钴离子, 铬离子, 单核/巨噬细胞, RANK, 人工假体

Abstract:

BACKGROUND: Similar to other prosthesis, metal-metal prosthesis would produce plenty of wear particles and metal ions, mainly presented as cobalt (Co 2+ )  and chromium (Cr 3+ ), which can lead to osteolysis, eventually, result in aseptic loosening.
OBJECTIVE: To observe the effect of Co 2+ and Cr 3+ ions on the cells viability and expression of RANK in rats monocyte-macrophage cells (RAW264.7) in vitro.
METHODS: Monocyte-macrophage cells (RAW264.7) were cultured in vitro, and then the cells were exposed to Co 2+ and Cr 3+ ions. The cell viability was assured by MTT test and the level of RANK mRNA was detected by semi-quantitative RT-PCR at different times.
RESULTS AND CONCLUSION: Compared to the control group, MTT test demonstrated that Co 2+ and Cr 3+ ions could decrease the cell activity of monocyte-macrophage cells obviously. When the cells were exposed to Co 2+ , Cr 3+ ions, compared to the control group, the mRNA expression of RANK of the metal ions group was increased at 12 hours (P < 0.05), reached its peak level at 24 hours (P < 0.05), and decreased at 48 hours than that of 24 hours (P < 0.05). The results revealed that metal ions have a cytotoxic effect on monocyte-macrophage cells, stimulate the expression of RANK, and have the potential of facilitating monocyte-macrophages cells transform into osteoclast-like cells.

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