中国组织工程研究

中国组织工程研究 ›› 2010, Vol. 14 ›› Issue (31): 5809-5812.doi: 10.3969/j.issn.1673-8225.2010.31.027

• 器官移植基础实验 basic experiments of organ transplantation • 上一篇    下一篇

肺及肺癌组织芯片PBK/TOPK蛋白表达：检测的可靠性

赵云飞，申  洪   

  1. 南方医院病理科，南方医科大学病理学系, 广东省广州市  510515
  • 出版日期:2010-07-30 发布日期:2010-07-30
  • 通讯作者: 申洪，教授，博士生导师，南方医科大学病理学系，广东省广州市 510515 Hongshen@fimmu.com
  • 作者简介:赵云飞★，男，1983年生，四川省资阳市人，汉族，南方医科大学在读硕士，主要从事定量病理学的研究。

Reliability of lung and lung cancer tissue microarray in detecting PBK/TOPK protein expression

Zhao Yun-fei, Shen Hong   

  1. Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou  510515, Guangdong Province, China
  • Online:2010-07-30 Published:2010-07-30
  • Contact: Shen Hong, Professor, Doctoral supervisor, Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China Hongshen@fimmu.com
  • About author:Zhao Yun-fei★, Studying for master’s degree, Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China

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摘要：

背景：组织芯片技术可高通量检测组织中的生物分子。但是组织芯片所用的组织很小，直径仅0.6 mm，且肿瘤细胞具有异质性，所以组织芯片检测结果是否可靠尚不清楚。
目的：比较石蜡组织芯片和对应组织常规石蜡切片PBK/TOPK 蛋白在正常成人肺泡Ⅱ型上皮细胞、人胚胎肺泡上皮细胞、肺癌原发灶和相应的淋巴结转移灶的表达差异，探讨组织芯片检测PBK/TOPK蛋白表达的可靠性。
方法：采用组织微阵列技术按照正常成人肺组织，胚胎肺组织，肺鳞癌原发灶及其相应淋巴结转移癌，肺腺癌原发灶及其相应淋巴结转移癌，肺小细胞癌原发灶及其相应淋巴结转移癌，肺大细胞癌原发灶及其相应淋巴结转移癌的顺序依次构建包含760点阵的石蜡组织芯片。应用免疫组化SP法，检测石蜡组织芯片和对应组织常规石蜡切片PBK/TOPK蛋白的表达。应用Leica Q500MC图像分析系统分别对石蜡组织芯片和常规石蜡切片上PBK/TOPK蛋白表达的阳性单位和阳性表达率进行定量测试。
结果与结论：组织芯片和对应常规切片相应细胞中PBK/TOPK 的阳性强度及阳性表达率基本相同，相对偏差不足0.6%，两者比较差异无显著性意义。结果说明石蜡组织芯片和常规组织切片检测PBK/TOPK蛋白表达结果高度一致；应用组织芯片检测肺癌、胚胎肺及正常肺组织中PBK/TOPK蛋白的表达结果可靠。

关键词: 肺癌, 组织芯片, PBK/TOPK, 免疫组化, 可靠性

Abstract:

BACKGROUND: Tissue microarray (TMA) technology can be used to detect biomelecule in tissues. However, the diameter of organization used in TMA is only 0.6 mm, and tumor cells has heterogeneous. Therefore, the reliability of test results of TMA remains unclear.
OBJECTIVE: To examine the reliability of TMA for detecting PBK/TOPK compared to the original paraffin sections containing normal human adult type Ⅱ alveolar epithelial cells, embryonic pneumocytes, primary focus of lung carcinoma and lymph node metastasis.
METHODS: The TMA technology was used to construct a paraffin tissue microarray containing 760 tissue cores, including normal adult human lung, embryonic pneumocytes, primary focus of lung carcinoma and lymph node metastasis, primary focus of lung adenocarcinoma and lymph node metastasis, primary focus of lung small cell carcinoma and lymph node metastasis, as well as primary focus of lung large cell carcinoma and lymph node metastasis. The immunohistochemical SP method was employed to determine the PBK/TOPK protein expression in TMA and original conventional paraffin sections. The positive unit of PBK/TOPK protein and positive rate of expression were assessed quantitatively with the Leica Q500MC image analysis system using results from the paired conventional full sections as controls.
RESULTS AND CONCLUSION: There was no significant difference in PBK/TOPK expression between TMA and paired conventional full sections in positive unit and positive expression rate, and the relative deviation was less than 0.6%. TMA detection of PBK/TOPK protein expression in lung carcinoma is highly concordant with the original paraffin sections. TMA is reliable in detecting PBK/TOPK protein expression of lung, embryonic lung and normal lung tissue.

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