中国组织工程研究

中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (25): 4054-4059.doi: 10.3969/j.issn.2095-4344.2098

• 干细胞综述 stem cell review • 上一篇    下一篇

细胞力学机械刺激对牙髓干细胞的影响

李峻青1，吴家媛1，2，3   

  1. 1遵义医科大学，贵州省遵义市  563000；2遵义医科大学附属口腔医院，贵州省遵义市  563000；3贵州省高等学校口腔疾病研究特色重点实验室，贵州省遵义市  563000

  • 收稿日期:2019-12-25 修回日期:2019-12-27 接受日期:2020-02-19 出版日期:2020-09-08 发布日期:2020-08-26
  • 通讯作者: 吴家媛，博士，教授，研究生导师，遵义医科大学，遵义医科大学附属口腔医院，贵州省高等学校口腔疾病研究特色重点实验室，贵州省遵义市 563000
  • 作者简介:李峻青，女，1993年生，贵州省遵义市人，汉族，遵义医科大学在读硕士，主要从事口腔内科方面的研究。
  • 基金资助:

    国家自然科学基金项目(81460102)；贵州省科技厅项目(黔科合J字LKZ[2013]11号)；贵州省高校优秀科技创新人才支持计划(合同号：KYJF(2014-8))；遵义市优秀青年科技创新人才培养项目(遵优青科【2018】8号)

Effect of mechanical stimulation on dental pulp stem cells 

Li Junqing1, Wu Jiayuan1, 2, 3   

  1. 1Zunyi Medical University, Zunyi 563000, Guizhou Province, China; 2Stomatological Hospital Affiliated to Zunyi Medical University, Zunyi 563000, Guizhou Province, China; 3Special Key Laboratory of Oral Diseases Research of Universities in Guizhou Province, Zunyi 563000, Guizhou Province, China

  • Received:2019-12-25 Revised:2019-12-27 Accepted:2020-02-19 Online:2020-09-08 Published:2020-08-26
  • Contact: Wu Jiayuan, MD, Professor, Master’s supervisor, Zunyi Medical University, Zunyi 563000, Guizhou Province, China; Stomatological Hospital Affiliated to Zunyi Medical University, Zunyi 563000,Guizhou Province, China; Special Key Laboratory of Oral Diseases Research of Universities in Guizhou Province, Zunyi 563000, Guizhou Province, China
  • About author:Li Junqing, Master candidate, Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 8146102; the Project of Science and Technology Department of Guizhou Province, No. J word LKZ[2013] 11 ; the Supporting Scheme of Distinguished Scientific Talents in Higher Education of Guizhou, China, No. KYJF(2014-8); the Foundation for Distinguished Young Talents in Technology Innovation of Zunyi, No. [2018]8 

摘要：

文题释义：

牙髓干细胞：通过GRONTHOS等对牙髓细胞的研究，发现一种具有与间充质干细胞相似免疫表型和形成矿化结节能力的细胞，称为牙髓干细胞，具有自我更新、多向分化和较强的克隆能力。

细胞力学：研究细胞在力学载荷作用下细胞膜和细胞骨架的变形、弹性常数、黏弹性、黏附力等力学性质，以及机械因素对细胞生长、发育、成熟、增殖、分化、衰老和死亡等的影响及其机制研究，是生物力学的一个前沿领域，也是组织工程学的一个重要组成部分。

 

摘要

背景：力学刺激对于机体内很多器官、组织的发育和损伤修复发挥重要的调控作用。除生物化学因素外，细胞力学等机械因素越来越被认为是影响牙髓干细胞行为和功能的关键调节因素。

目的：综述细胞力学刺激对牙髓干细胞生物学行为的作用及影响。

方法：通过检索PubMed、Embase、Medline、CNKI数据库，分别以“牙髓干细胞，机械压力，机械张力，剪切力，压应力，细胞增殖，成骨分化”为中文检索词和“human dental pulp stem cells(hDPSCs)，mechanical strain，mechanical stretch，mechanical tension，shear stress，cell proliferation，osteogenesis differentiation”为英文检索词进行检索，选取与细胞力学机械刺激参与影响牙髓干细胞增殖、分化的56篇文献进行综述。

结果与结论：细胞力学机械刺激是影响细胞增殖、分化、蛋白表达和凋亡的重要生物学因素。牙髓干细胞是牙髓组织中的间充质干细胞，其生物学行为也受到细胞力学机械刺激的影响。细胞力学刺激参与牙髓干细胞的增殖、成牙/成骨分化，并且当牙本质受到流体流动力作用时，会激活其机械感受器来调节并维持牙齿的完整性。介导牙髓干细胞生物学行为的信号通路包括MAPK、Wnt、Akt及BMP-7、Nrf2/HO-1等，通过调控这些信号通路进而对牙髓干细胞增殖及成牙/成骨分化发挥不同程度的促进及抑制作用。

ORCID: 0000-0001-6191-6539(李峻青)

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词:

牙髓干细胞, 细胞增殖,  成骨分化,  牙源性牙本质干细胞,  力学性能,  细胞力学,  生物学行为,  牙再生组织工程

Abstract:

BACKGROUND: Mechanical stimulation plays a necessary regulatory role in developing and repairing many organs and tissues in the human body. Except for biochemical factors, mechanical factors are considered as key regulatory factors that affect the behavior and function of dental pulp stem cells.



OBJECTIVE: To review the role and effect of cellular mechanical stimulation on the biological behavior of dental pulp stem cells.

METHODS: PubMed, Embase, Medline and CNKI databases were searched for relevant literatures using the keywords of “human dental pulp stem cells (hDPSCs), mechanical strain, mechanical stretch, mechanical tension, shear stress, cell proliferation, osteogenesis differentiation” in English and Chinese, respectively. Fifty-six articles were finally eligible for review, which were closely related to the proliferation and differentiation of dental pulp stem cells under cellular mechanical stimulation.

RESULTS AND CONCLUSION: Cellular mechanical stimulation is an important biological factor affecting cell proliferation, differentiation, apoptosis and protein expression. Dental pulp stem cells are mesenchymal stem cells derived from the dental pulp tissue, and their biological behaviors are also affected by cellular mechanical stimulation. Cellular mechanical stimulation is involved in the proliferation, odontogenesis/osteogenesis of dental pulp stem cells. When the dentin is subjected to fluid flow forces, mechanoreceptors are activated to regulate and maintain the integrity of tooth structure. Signal pathways that mediate the biological behavior of dental pulp stem cells include MAPK, Wnt, Akt, BMP-7, and Nrf2/HO-1, which are involved in promoting and inhibiting the proliferation and odontogenesis/osteogenesis of dental pulp stem cells to varying degrees.

Key words:

dental pulp stem cell,  cell proliferation,  osteogenic differentiation, odontogenic dentin stem cells,  mechanical properties,  cellular mechanics,  biological behavior,  tooth regeneration and tissue engineering 

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