中国组织工程研究

中国组织工程研究 ›› 2020, Vol. 24 ›› Issue (28): 4429-4436.doi: 10.3969/j.issn.2095-4344.2315

• 生物材料综述 biomaterial review •    下一篇

不同材料因素影响骨髓间充质干细胞的增殖及成骨分化

王旸昊，王伟舟，段  浩，钟宗雨，李晓壮，唐志宏，何  飞   

  1. 昆明医科大学第一附属医院，云南省昆明市  650032

  • 收稿日期:2019-12-07 修回日期:2019-12-10 接受日期:2020-02-12 出版日期:2020-10-08 发布日期:2020-08-29
  • 通讯作者: 何飞，主任医师，昆明医科大学第一附属医院，云南省昆明市650032
  • 作者简介:王旸昊，男，1996年生，重庆市人，汉族，昆明医科大学在读硕士，主要从事骨科研究。
  • 基金资助:
    国家自然科学基金项目(31460244)；云南省重大科技专项(生物医疗)(2019ZF002)；云南省科技计划项目省应用基础研究(2015FA002)

Different material factors affect the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells

Wang Yanghao, Wang Weizhou, Duan Hao, Zhong Zongyu, Li Xiaozhuang, Tang Zhihong, He Fei   

  1. First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China

  • Received:2019-12-07 Revised:2019-12-10 Accepted:2020-02-12 Online:2020-10-08 Published:2020-08-29
  • Contact: He Fei, Chief physician, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • About author:Wang Yanghao, Master candidate, First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 31460244 ; Major Science and Technology Projects of Yunnan Province (Biomedicine), No. 2019ZF002; the Applied Basic Research Project of Yunnan Science and Technology Department Foundation, No. 2015FA002

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摘要：

文题释义：

表面微观形貌：材料表面所具有的所有微观几何形状及相关物理量统称为表面微观形貌，包括孔径大小、孔径率、表面凹凸程度、底物刚度、弹性模量、表面能等方面，同一种材料不同的表面微观形貌将会对材料表面的物质产生不同的影响。

表面能/润湿性：表面能是指恒温、恒压、恒组成情况下可逆地增加物系表面积须对物质所做的非体积功，润湿性是指一种液体在一种固体表面铺展的能力或倾向性。

背景：在利用组织工程技术治疗骨缺损或骨损伤的过程中，生物材料对移植的骨髓间充质干细存活率与增殖分化潜能有影响。

目的：对生物材料如何影响骨髓间充质干细胞的增殖及成骨分化的研究进展进行综述，指导材料合理应用。

方法：应用计算机检索中国知网、PubMed、Web of science及万方数据库，检索词为“骨髓间充质干细胞、成骨分化、生物材料、微观形貌、Bone marrow mesenchymal stem cells、Osteogenic differentiation、Biological materials、The microstructure”，根据标准纳入不同生物材料因素影响骨髓间充质干细胞增殖及成骨分化的文献。

结果与结论：①金属材料具有良好的生物相容性、骨传导性、机械性能，非金属材料具有良好的生物相容性、骨传导性、再吸收性及三维塑形性；②材料的表面微观形貌因素众多，即使是同一种材料，表面能/润湿性越高越利于骨髓间充质干细胞的增殖与成骨分化，表面粗糙程度相对越高越利于细胞的增殖、黏附及分化，孔径直径越大、孔径率越小越利于细胞的成骨分化，底物刚度相对高及弹性模量相对大有利于骨髓间充质干细胞的成骨分化，以上材料的各种因素等都能够影响骨髓间充质干细胞的增殖及成骨作用，这些新的研究进展进一步提高了骨髓间充质干细胞种植于各类生物材料用于临床治疗的应用。

ORCID: 0000-0001-8325-9867(王旸昊)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 表面微观形貌, 非金属材料,  金属材料,  骨髓间充质干细胞,  成骨分化,  增殖分化,  组织工程,  骨组织材料

Abstract:

BACKGROUND: In the treatment of bone defect or bone injury by tissue engineering, biomaterials affect the survival rate, proliferation and differentiation of bone marrow mesenchymal stem cells.

OBJECTIVE: To review the research progress regarding how biomaterials affect the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells, and to guide the rational application of biomaterials.

METHODS: A computer-based online search of CNKI, PubMed, Web of Science, and Wanfang database with the search terms “Bone marrow mesenchymal stem cells, Osteogenic differentiation, Biological materials, The microstructure". The eligible literatures regarding the effects of different biomaterials on the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells were included.  

RESULTS AND CONCLUSION: (1) Metallic materials have the advantages of good biocompatibility, bone conductivity, and mechanical performance. Non-metallic materials exhibit good biocompatibility, bone conductivity, reabsorption, and three-dimensional shaping. (2) There are many factors that affect the surface microstructure of the biomaterial. As for the same biomaterial, greater surface energy/ wettability leads to better proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells; greater roughness better promotes the proliferation, adhesion, and differentiation of bone marrow mesenchymal stem cells; larger pore diameter and lower pore diameter rate are more prone to promote the osteogenic differentiation of bone marrow mesenchymal stem cells; greater substrate rigidity and elastic modulus better facilitate the osteogenic differentiation of bone marrow mesenchymal stem cells. The abovementioned factors of the biomaterials affect the proliferation and osteogenic differentiation of bone marrow mesenchymal stem cells. These findings help promote the application of biomaterials seeded with bone marrow mesenchymal stem cells in the clinic.

Key words: surface microstructure, non-metallic material, metallic material, bone marrow mesenchymal stem cells, osteogenic differentiation,  , proliferation and differentiation, tissue engineering, bone tissue material

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