中国组织工程研究

中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (32): 5177-5182.doi: 10.3969/j.issn.2095-4344.2017.32.016

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

颈椎病模型大鼠接受电脉冲联合中药敷贴：活血化瘀作用及机制

朱传武1，杨  芳2   

  1.  (1南方医科大学中医药学院，广东省广州市  510515；2南方医科大学南方医院药剂科， 广东省广州市  510515)
  • 收稿日期:2017-08-07 出版日期:2017-11-18 发布日期:2017-11-15
  • 通讯作者: 杨芳，硕士，主管药师，南方医科大学南方医院药剂科， 广东省广州市 510515
  • 作者简介:朱传武，男，1974年生，安徽省合肥市人，汉族，2004年第一军医大学毕业，硕士，讲师，主治医师，主要从事中药药理研究。
  • 基金资助:

    广东省中医药局基金(20130601)；广州市海珠区科技计划项目(2013-cg-34)

Chinese herbal compound combined with electrical pulse for cervical spondylosis in a rat model: microcirculation improvement and the underlying mechanism

Zhu Chuan-wu1, Yang Fang2   

  1.  (1School of TCM, Southern Medical University, Guangzhou 510515, Guangdong Province, China; 2Department of Pharmacy, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China)
  • Received:2017-08-07 Online:2017-11-18 Published:2017-11-15
  • Contact: Yang Fang, Master, Pharmacist-in-charge, Department of Pharmacy, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • About author:Zhu Chuan-wu, Master, Lecturer, Attending physician, School of TCM, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Supported by:

    the Foundation of Traditional Chinese Medicine Bureau of Guangdong Province, No. 20130601; the Science and Technology Program of Haizhu District in Guangdong Province, No. 2013-cg-34

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摘要：

文章快速阅读：


文题释义：
中频电脉冲：中频电疗法(Medium frequency electrotherapy)，应用频率为1 000-100 000 Hz的脉冲电流治疗疾病的方法，称为中频电疗法。临床常用的有干扰电疗法、调制中频电疗和等幅正弦中频(音频)电疗法3种。近年来，随着计算机技术的应用，已有电脑中频电疗机、电脑肌力治疗机问世，并应用于临床。
全血黏度：全血黏度是一个综合性指数，它是血浆黏度、血细胞压(比)积、红细胞变形性和聚集能力、血小板和白细胞流变特性的综合表现，是血液随不同流动状况(切变率)及其他条件而表现出的黏度，切变率低时血黏度高，随切变率的逐渐升高黏度逐渐下降，最后趋向一个平稳的数值。
摘要
背景：乌龙丹系国家级名老中医臧堃堂教授临床经验方，在临床上常用于治疗脑梗死、短暂性脑缺血发作、脑出血恢复期、颈椎病等缺血性脑病，效果显著使用安全。
目的：观察中药复方联合电脉冲对其血液流变性的影响，探讨其作用机制。
方法：雄性SD大鼠共24只，被随机分为3组：正常组、治疗组及模型组各8只。除正常组外其余大鼠制作动静力失衡型颈椎病动物模型，造模后3 d治疗组大鼠给予电脉冲联合中药治疗：将棉布经中药液浸泡后，拧至半干放置于治疗组大鼠颈部，将2块电极板覆盖其上固定电极板进行电脉冲治疗，每天20 min，20 d为1个疗程；模型组不做此处理。用血液黏度测试仪测定全血黏度和血浆黏度；用红细胞变形仪测定红细胞最大变形指数；用微量高速离心法测定红细胞压积。
结果与结论：①与正常组比较，模型组在1 s-1、5 s-1、30 s-1、150 s-1四种切变率下全血黏度指标均显著升高(P < 0.01)；与模型组比较，治疗组在1 s-1、5 s-1、30 s-1、150 s-1四种切变率下全血黏度指标均显著降低(P < 0.01－0.05)；②与正常组和治疗组比较，模型组血浆黏度均显著升高(P < 0.01)；③与正常组比较，模型组和治疗组红细胞聚集指数均显著升高(P < 0.01)，治疗组和模型组无显著性差异；④治疗组红细胞压积较模型组好转但依旧显著高于正常组；⑤正常组和治疗组血小板聚集率均显著低于模型组(P < 0.01)；⑥治疗组血小板聚集率显著高于正常组(P < 0.01)；⑦结果说明，中药复方联合电脉冲对大鼠颈椎病动物模型具有明显的活血化瘀作用，其机制与该方能使聚集的红细胞和血小板解聚等有关。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0001-9140-3865(朱传武)

关键词: 组织构建, 组织工程, 电脉冲, 中药, 颈椎病, 动物模型

Abstract:

BACKGROUND: Wulong Dan, a clinical prescription designed by Professor Zang Kun-tang, has been used to treat ischemic encephalopathies including cerebral infarction, transient ischemic attack, convalescent stage of cerebral hemorrhage and cervical spondylosis, showing overt curative efficacy and safety.
OBJECTIVE: To investigate the therapeutic effect of Chinese herbal compound combined with electrical pulse on hemorrheology and the underlying mechanism.
METHODS: Twenty-four male Sprague-Dawley rats were randomized into normal, treatment and model groups (n=8 per group). The models of cervical spondylosis induced by unbalanced dynamic and static forces were established in the rats in the latter two groups. At 3 days after modeling, the rats in the treatment group received Chinese herbal compound combined with electrical pulse: a damp-dry cotton cloth after immersed in Chinese herbal was put on the rat neck which was then covered with two electrode plates and fixed for electrical pulse therapy, 20 minutes daily, 20 days a course. The model group was given no treatment. The whole blood viscosity and plasma viscosity were detected using blood viscosity tester; the maximum erythrocyte deformation index was detected by erythrocyte detector; the hematocrit was detected using high speed centrifugation.
RESULTS AND CONCLUSION: Compared with the normal group, the whole blood viscosity at shear rates of 1, 5, 30 and 150 s-1 was significantly increased in the model group, and the whole blood viscosity in the model group was significantly higher than that in the treatment group (P < 0.01-0.05). Compared with the normal and treatment groups, the plasma viscosity was significantly increased in the model group (P < 0.01). There was significantly elevated erythrocyte aggregation in the model and treatment groups compared with the normal group (P < 0.01), but there was no significant difference in the erythrocyte aggregation between model and treatment groups. The hematocrit in the treatment group was significantly improved compared with the model group, but it was still significantly higher than that in the normal group. The order of platelet aggregation rate was as follows: the model group > treatment group > normal group (P < 0.01). These results suggest that Chinese herbal compound combined with electrical pulse can significantly improve microcirculation by aggregating red blood cells and depolymerizing palates in a rat model of cervical spondylosis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Pulse Therapy, Drug, Drugs, Chinese Herbal, Cervical Vertebrae, Tissue Engineering

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