中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (18): 2913-2919.doi: 10.3969/j.issn.2095-4344.2017.18.020

• 药物控释材料 drug delivery materials • 上一篇    下一篇

新型组织工程化改性胶原-壳聚糖复合支架制备与神经细胞的相容性

陆  江1,2,3,陈绚丽4,唐核心1,2,佘  纳2,朱  垚1,李思星1,王天颖1,张国辉1
  

  1. 湖北医药学院,1第一临床学院,2麻醉学研究所, 4基础医学研究所,湖北省十堰市  442000;3湖北医药学院附属太和医院麻醉科,湖北省十堰市  442000
  • 收稿日期:2017-02-21 出版日期:2017-06-28 发布日期:2017-07-07
  • 通讯作者: 张国辉,湖北医药学院第一临床学院,湖北省十堰市 442000
  • 作者简介:陆江,男,广西壮族自治区河池市人,瑶族,2010年广西大学毕业,博士,讲师,主要从事医学胚胎工程研究。
  • 基金资助:

    国家教育部2012年度国家级大学生创新创业训练计划项目(201210929004);国家教育部2016年度国家级大学生创新创业训练计划项目(201610929005);十堰市科学技术研究与开发项目(14Y13);2016年度湖北医药学院教学研究项目(2016027);湖北医药学院高层次人才科研启动金(2011QDZR-1,2011QDZR-2);湖北医药学院2015年度“十二五规划”学科建设学科英才成长资助项目(2015);2016年度湖北医药学院附属太和医院科学研究基金(2016JJXM001)

A novel tissue-engineered crosslinked collagen-chitosan scaffold and its biocompatibility with nerve cells

Lu Jiang1, 2, 3, Chen Xuan-li4, Tang He-xin1, 2, She Na2, Zhu Yao1, Li Si-xing1, Wang Tian-ying1, Zhang Guo-hui1 
  

  1. 1First Clinical College, 2Institute of Anesthesiology, 4Basic Medical Institute, Hubei University of Medicine, Shiyan 442000, Hubei Province, China; 3Department of Anesthesiology, Affiliated Taihe Hospital of Hubei University of Medicine, Shiyan 442000, Hubei Province, China
  • Received:2017-02-21 Online:2017-06-28 Published:2017-07-07
  • Contact: Zhang Guo-hui, First Clinical College, Hubei University of Medicine, Shiyan 442000, Hubei Province, China
  • About author:Lu Jiang, M.D., Lecturer, First Clinical College, Hubei University of Medicine, Shiyan 442000, Hubei Province, China; Institute of Anesthesiology, Hubei University of Medicine, Shiyan 442000, Hubei Province, China; Department of Anesthesiology, Affiliated Taihe Hospital of Hubei University of Medicine, Shiyan 442000, Hubei Province, China
  • Supported by:
    the National College Student Innovation and Entrepreneurship Training Program of Education Ministry of China in 2012, No. 201210929004; the National College Student Innovation and Entrepreneurship Training Program of Education Ministry of China in 2016, No. 201610929005; the Scientific and Technological Project of Shiyan City of Hubei Province, No. 14Y13; the Educational Scientific Foundation of Hubei University of Medicine in 2016, No. 2016027; the Natural Science Research Project of Hubei University of Medicine, No. 2011QDZR-1, 2011QDZR-2; Fund of Disciplines Construction of Hubei University of Medicine in 2015, No. 2015; and the Scientific Foundation of Affiliated Taihe Hospital of Hubei University of Medicine in 2016, No. 2016JJXM001

摘要:

文章快速阅读:

 

文题释义:
改性胶原-壳聚糖复合支架:体外构建鼠尾源性胶原-壳聚糖复合支架,然后用1-乙基-3-(3-二甲基氨基丙基)-碳化二亚胺、N-羟基琥珀酰亚胺对其进行化学改性,使其成为性能良好的生物支架材料。
支架支撑种子细胞于坐骨神经缺损部位生长:支架内生长分化的神经细胞联合移植到受损的坐骨神经部位,神经细胞可以继续存活并成熟,预计有可能会对坐骨神经损伤修复产生积极意义。
 
背景:周围神经缺损的治疗在再生医学领域仍然具有挑战性,支架材料的应用被认为是有前途的探索方向之一,理想的支架材料应是安全、耐用和缺乏抗原性的。
目的:探讨制备一种新型的组织工程化改性胶原-壳聚糖支架,并探索其在大鼠横断坐骨神经模型中用作桥梁修复神经损伤的可能。
方法:先制备出胶原-壳聚糖复合支架,然后用1-乙基-3-(3-二甲基氨基丙基)-碳化二亚胺,N-羟基琥珀酰亚胺对支架进行化学改性即交联。接着将神经干细胞种植于支架上,之后连同支架-种子细胞在体内植入坐骨神经缺损部位。
结果与结论:制得的变性交联胶原-壳聚糖复合支架具有三维立体网状结构。将支架-种子细胞移植4周后,可在损伤的坐骨神经中发现S-100神经丝蛋白200表达。结果表明这种组织工程化改性交联胶原-壳聚糖复合支架具有良好的细胞相容性。

关键词: 生物材料, 材料相容性, 胶原, 壳聚糖, 支架, 改性, 超微结构, 孔隙率, 降解速率

Abstract:

BACKGROUND: Treatment for peripheral nerve injury is still a challenge in the field of regenerative medicine. The use of scaffold materials is considered to be one of the promising opportunities to settle these defects. The ideal scaffold material should be safe and durable, but have no antigenicity.
OBJECTIVE: To develop a novel tissue-engineered collagen-chitosan composite scaffolds under modification in attempt to repair sciatic nerve injury in rats.
METHODS: First the collagen-chitosan composite scaffold was prepared, and then cross-linked with 1-ethyl-3-(3-2 methyl amino propyl)-WC 2 imide (EDC) and N-hydroxy succinimide (NHS). After modification, nerve cells were seeded onto the scaffold. Finally, the cell-scaffold composite was implanted into the injured sciatic nerve.
RESULTS AND CONCLUSION: The EDC/NHS-crosslinked collagen-chitosan scaffold showed a three-dimensional reticular structure. Four weeks after the cell-scaffold composite implantation, neurofilament protein 200 was found to express in the injured sciatic nerve. This indicates that the crosslinked collagen-chitosan scaffold has good cytocompatibility.

Key words: Biocompatible Materials, Collagen, Chitosan, Tissue Engineering

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