中国组织工程研究 ›› 2017, Vol. 21 ›› Issue (18): 2852-2857.doi: 10.3969/j.issn.2095-4344.2017.18.011

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

新型蜂巢样聚己内酯-硅酸钙复合晶体材料修复颅骨极限缺损

宋  兵,廖哲霆,陈宇璠,赵  亮   

  1. 南方医科大学南方医院关节与骨病外科,广东省广州市  510515
  • 收稿日期:2017-04-15 出版日期:2017-06-28 发布日期:2017-07-07
  • 通讯作者: 赵亮,主任医师,南方医科大学南方医院关节与骨病外科,广东省广州市 510515
  • 作者简介:宋兵,男,1990年生,山东省德州市人,汉族,南方医科大学硕士研究生在读,主要从事骨组织,软骨组织再生研究。
  • 基金资助:

    广东省自然科学基金(2014A030313275,s2013010014253);南方医科大学“国家杰出青年培养计划”项目

Bone regeneration via a novel honeycomb-like polycaprolactone-calcium silicate crystal compound scaffold in extreme-sized cranial defects

Song Bing, Liao Zhe-ting, Chen Yu-fan, Zhao Liang
  

  1. Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Received:2017-04-15 Online:2017-06-28 Published:2017-07-07
  • Contact: Zhao Liang, Chief physician, Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • About author:Song Bing, Studying for master’s degree, Department of Orthopedic Surgery, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Supported by:
     the Natural Science Foundation of Guangdong Province, No. 2014A030313275, s2013010014253; the National Outstanding Youth Culture Plan of Southern Medical University

摘要:

文章快速阅读:

 

文题释义:
聚己内酯:是一种比较成熟的高分子聚合材料,具有良好的生物相容性,低毒性且降解时间较快,已被广泛用作生物支架及药物载体作用,但其作为高分子材料,不可避免地引起机体炎性反应。聚己内酯与其他天然生物材料一起构建生物支架,可增加其生物相容性,促进机体组织再生。
新型蜂巢样聚己内酯-硅酸钙复合晶体材料:是一种由硅酸盐与β-磷酸三钙高温煅烧形成的生物材料,并与聚己内酯材料混合后3D打印形成蜂巢样晶体颗粒材料,具有优良的生物活性、生物相容性及骨激发性。
 
背景:聚己内酯作为高分子材料的生物相容性较差,需将其与其他天然生物材料复合增加生物相容性,促进机体组织再生。
目的:观察新型蜂巢样聚己内酯-硅酸钙复合晶体材料在SD大鼠颅骨缺损中的成骨修复作用。
方法:取18只SD大鼠,制作颅骨极限缺损模型,随机分3组干预,空白组不植入任何材料,对照组植入普通聚己内酯-硅酸钙复合晶体材料,实验组植入新型蜂巢样聚己内酯-硅酸钙复合晶体材料。植入6周后,进行骨缺损处X射线扫描、Micro-CT三维重建及组织学分析。
结果与结论:①X射线扫描:3组大鼠颅骨缺损变小,骨折线模糊,边缘骨密度增高,实验组、对照组新生骨面积百分比明显大于空白组,且实验组新生骨面积百分比大于对照组;②Micro-CT三维重建:空白组新生骨主要分布在缺损两侧,对照组、实验组新生骨分布于整个颅骨骨缺损处;与空白组比较,实验组、对照组均表现出更强的再生骨能力(P < 0.05),且以实验组成骨能力最强(P < 0.05);③组织学分析:3组均可见骨缺损处有不同程度新生骨长入,实验组新生骨组织及新生血管密度明显高于对照组、空白组(P < 0.05);④结果表明:新型蜂巢样聚己内酯-硅酸钙复合晶体材料在颅骨缺损生物环境中具有明显的促成骨作用。

关键词: 生物材料, 骨生物材料, 聚己内酯-硅酸钙复合晶体材料, 骨缺损, 颅骨再生, 骨修复, 聚己内酯, 广东省自然科学基金

Abstract:

BACKGROUND: Polycaprolactone as a polymer material has poor biocompatibility, and needs to be combined with other natural biological materials to increase biocompatibility, thereby promoting tissue regeneration.
OBJECTIVE: To develop a novel honeycomb-like polycaprolactone-calcium silicate crystal compound scaffold, and observe its osteogenic effects in Sprague-Dawley rats with skull defects.
METHODS: Eighteen Sprague-Dawley rats were used to make skull defect models and randomized into three groups: blank control group with no implantation, control group with implantation of normal polycaprolactone-calcium silicate crystal compound scaffold, and experimental group with implantation of the novel honeycomb-like polycaprolactone- calcium silicate crystal compound scaffold. Six weeks after implantation, bone regeneration effect in the defect region measured via X-ray scanning, Micro-CT three-dimensional reconstruction, and histological analysis.
RESULTS AND CONCLUSION: (1) X-ray scan: in all the rats, the size of bone defect was reduced, the fracture line became vague, and the marginal bone density was increased. The percentage of new bone area was highest in the experimental group, successively followed by the control group and blank control group. (2) Micro-CT three-dimensional reconstruction: new bones in the blank control group were mainly distributed on the both sides of the defect, but those in the control and experimental groups distributed in the defect region. The bone regeneration capacity was ranked as follows: experimental group > control group > blank control group (P < 0.05). (3) Histological analysis: new bone ingrowth was visible in all the three groups to different extents. Compared with the other two groups, new bone formation and microvessel density were significantly higher in the experimental group (P < 0.05). To conclude, this novel honeycomb-like polycaprolactone-calcium silicate crystal compound scaffold can obviously promote bone formation in the skull defect region. 

Key words: Skull, Bone Regeneration, Tissue Engineering

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