中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (46): 6854-6860.doi: 10.3969/j.issn.2095-4344.2016.46.002

• 骨组织构建 bone tissue construction • 上一篇    下一篇

甲状旁腺激素1-34、阿仑膦酸钠、辛伐他汀治疗大鼠骨质疏松效果的比较

穆树林1，张  柳1，田发明2，穆树敏1，孙丽霞1，褚立明1   

  1. 1华北理工大学附属医院，河北省唐山市  063000；2华北理工大学医学实验研究中心，河北省唐山市  063000
  • 出版日期:2016-11-11 发布日期:2016-11-11
  • 通讯作者: 田发明，博士，副教授，硕士生导师，华北理工大学医学实验研究中心，河北省唐山市 063000
  • 作者简介:穆树林，男，1974年生，河北省唐山市人，汉族，2009年华北煤炭医学院研究生部，硕士，主治医师，主要从事骨质疏松与骨质疏松性骨折的临床与基础研究。
  • 基金资助:

    河北省卫计委科技计划项目(20150086；20130377)

Comparison of the effects of parathyroid hormone (1-34), alendronate sodium and simvastatin on osteoporotic rats

Mu Shu-lin1, Zhang Liu1, Tian Fa-ming2, Mu Shu-min1, Sun Li-xia1, Chu Li-ming1   

  1. 1Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China; 2Medical Research Center, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • Online:2016-11-11 Published:2016-11-11
  • Contact: Tian Fa-ming, M.D., Associate professor, Master’s supervisor, Medical Research Center, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • About author:Mu Shu-lin, Master, Attending physician, Affiliated Hospital, North China University of Science and Technology, Tangshan 063000, Hebei Province, China
  • Supported by:

    the Science and Technology Planning Project of Hebei Provincial Health and Family Planning Commission, No. 20150086; 20130377

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摘要：

文章快速阅读：

文题释义：
甲状旁腺激素：是一种由颈部的甲状旁腺分泌，具有84个氨基酸的多肽类激素。主要作用在骨骼、肾脏，增加血液中的钙离子浓度。
辛伐他汀：辛伐他汀(simvastatin)是他汀类(statin)的降血脂药物，用于控制血液中胆固醇的含量以及预防心血管疾病。辛伐他汀是土曲霉发酵产物的合成衍生物。
摘要
背景：在应用骨吸收类药物治疗骨质疏松性骨折尚存争议的背景下，对新型药物尤其是促进骨形成类药物的开发显得愈发重要和迫切。
目的：比较甲状旁腺激素1-34、阿仑膦酸钠、辛伐他汀对卵巢切除大鼠骨丢失的作用。
方法：3月龄雌性SD大鼠30只，随机分为5组，每组6只，除假手术组外，其余大鼠接受双侧卵巢切除术，术后3个月开始分别给予安慰剂(卵巢切除+安慰剂组)、甲状旁腺激素1-34(20 μg/kg，5 d/周，卵巢切除+甲状旁腺激素组)、阿仑膦酸钠[70 μg/(kg•周)，卵巢切除+阿仑膦酸钠组]、辛伐他汀[5 mg/(kg•d)，卵巢切除+辛伐他汀组]，3个月后处死大鼠，双能X射线法检测大鼠L4腰椎骨密度、骨组织形态计量学分析其松质骨骨量和微观结构，取L5腰椎行压缩试验，分析其最大载荷、弹性模量等生物力学性能。
结果与结论：①骨密度：各组骨密度由高到低依次为卵巢切除+甲状旁腺激素组、假手术组、卵巢切除+阿仑膦酸钠组、卵巢切除+辛伐他汀组和卵巢切除+安慰剂组，除卵巢切除+甲状旁腺激素组与假手术组无显著差别外，任意2组间比较均差异有显著性意义(P < 0.05)；②骨组织形态计量学分析：卵巢切除+安慰剂骨小梁相对体积显著低于其余各组，卵巢切除+甲状旁腺激素组显著高于卵巢切除+阿仑膦酸钠组和卵巢切除+辛伐他汀组；③生物力学性能：卵巢切除+安慰剂组显著低于其余各组，假手术组和卵巢切除+甲状旁腺激素组显著高于卵巢切除+辛伐他汀和卵巢切除+阿仑膦酸钠组。④结果说明，研究采用的剂量和干预周期下，3种药物对去卵巢大鼠骨质疏松的疗效由大到小依次为甲状旁腺激素1-34、阿仑膦酸钠、辛伐他汀。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0002-2214-4668(穆树林)

关键词: 组织构建, 骨组织工程, 甲状旁腺激素1-34, 卵巢切除, 阿仑磷酸钠, 辛伐他汀, 骨密度, 生物力学

Abstract:

BACKGROUND: Bone resorption drugs in the treatment of osteoporotic fracture are still controversial. The development of new drugs, especially drugs in the promotion of bone formation, become more important and urgent. 
OBJECTIVE: To compare the effects of parathyroid hormone (1-34), alendronate sodium and simvastatin on bone loss in ovariectomized rats.
METHODS: Thirty female Sprague-Dawley rats aged 3 months were randomized into five groups of six animals in each group. All rats but those in sham group received dual ovariectomy (OVX) and treated by vehicle (OVX+V), parathyroid hormone (1-34) (5 days per week at a dose of 20 μg/kg, OVX+P), alendronate sodium (70 μg/kg/wk, OVX+A), simvastatin (5 mg/kg/d, OVX+S). Three months later, all rats were sacrificed. Dual energy X-ray method was used to measure bone density of L4 vertebra. Bone histomorphometry was utilized to analyze cancellous bone mass and bone microstructure. Compression test was performed on the L5 vertebra for the maximum loading and elastic modulus.  
RESULTS AND CONCLUSION: (1) Bone density: The bone density from high to low levels was OVX+P group, sham group, OVX+A group, OVX+S group, and OVX+V group. No significant difference in bone density was detectable between the OVX+P and sham groups, but significant differences in bone density were determined between any other two groups (P < 0.05). (2) Bone histomorphometry: Relative volume of trabecular bone was significantly lower in the OVX+V group than in other groups. Relative volume of trabecular bone was remarkably higher in the OVX+P group than in the OVX+A group and OVX+S group. (3) Biomechanical properties: Biomechanical properties were noticeably lower in the OVX+V group than in other groups. Biomechanical properties were remarkably higher in the sham group and OVX+P group than in the OVX+S group and OVX+A group. (4) These findings indicated that under the dosage and intervention period used in the present study, parathyroid hormone (1-34), alendronate sodium and simvastatin showed anti-osteoporosis effect on OVX rats in a descending manner.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoporosis, Ovariectomy, Bone Density, Tissue Engineering