中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (38): 5629-5635.doi: 10.3969/j.issn.2095-4344.2016.38.001

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials •    下一篇

羟基丁酸-羟基辛酸共聚体/胶原骨软骨一体化支架修复膝关节软骨损伤

段新虎   

  1. 山西医科大学，山西省太原市   030001
  • 收稿日期:2016-06-20 出版日期:2016-09-16 发布日期:2016-09-16
  • 作者简介:段新虎，男，1988年生，山西省稷山县人，汉族，2016年山西医科大学毕业，硕士，主要从事骨与软骨组织工程研究。

Poly(hydroxybutyrate-co-hydroxyoctanoate/collagen) osteochondral tissue-engineered scaffold used for repair of knee cartilage injury

Duan Xin-hu   

  1. Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
  • Received:2016-06-20 Online:2016-09-16 Published:2016-09-16
  • About author:Duan Xin-hu, Master, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China

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379

被引次数

3

摘要：

文章快速阅读：

文题释义：
关节软骨损伤：由炎症、创伤、退行性病变引起关节表面透明软骨层部分或全层损伤，出现疼痛、活动功能障碍等一系列临床症状。
骨软骨一体化支架：该支架是组织工程领域应用的载体构架，以某种生物材料为基础原料，仿照关节结构及生理功能，采用一定的技术手段制备的具有骨层、骨软骨中间界面层、软骨层3层结构的载体结构。

背景：由于骨软骨组织自身复杂的生理学特性，关节软骨损伤的临床修复效果难以令人满意，组织工程学方法和手段修复膝关节软骨缺损成为一种新的治疗模式，为临床修复骨软骨缺损提供了新的思路。
目的：观察羟基丁酸-羟基辛酸共聚体/胶原蛋白仿生一体化支架对兔膝关节软骨损伤的修复情况。
方法：以羟基丁酸与羟基辛酸为基础材料，混合一定比例胶原蛋白，采用溶剂浇铸/颗粒沥滤法，制备骨软骨一体化支架，接种分离、培养的种子细胞。选取4周龄健康新西兰大白兔24只，复合麻醉后，股骨髁关节面部位用电动骨钻造成关节软骨缺损(直径4.5 mm，深度5 mm)，处理后随机分为3组，空白组损伤处直接缝合；对照组损伤处植入单纯支架；实验组损伤处植入支架-软骨细胞复合体。分别于术后8，20周取出股骨髁后，通过大体及组织学观察缺损处修复情况。
结果与结论：①8周：实验组缺损处见透明薄膜组织覆盖，细胞小、不规则；对照组缺损较明显，纤维组织为主；空白组缺损无明显修复痕迹；②20周：实验组软骨缺损处以透明软骨样组织覆盖，细胞排列规则；对照组缺损处白色膜状组织覆盖，基底部分及边缘可见较多软骨细胞存在；空白组缺损可见少量新生组织；③结果表明：复合种子细胞的羟基丁酸-羟基辛酸共聚体/胶原骨软骨一体化支架有利于关节软骨缺损修复。
ORCID: 0000-0002-8223-2758(段新虎)

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

关键词: 生物材料, 软骨生物材料, 羟基丁酸-羟基辛酸共聚体, 软骨, 胶原蛋白, 组织工程支架, 膝关节, 关节软骨缺损

Abstract:

BACKGROUND: Due to the complex physiological characteristics of the osteochondral tissue, the clinical repair of knee cartilage injury often has dissatisfied outcomes. Tissue engineering methods and tools provide a new idea for osteochondral repair.
OBJECTIVE: To observe the effect of poly(hydroxybutyrate-co-hydroxyoctanoate/collagen) osteochondral tissue-engineered scaffold on the repair of articular cartilage injury in a rabbit.
METHODS: The poly(hydroxybutyrate-co-hydroxyoctanoate/collagen) osteochondral tissue-engineered scaffold was prepared by solvent casting/particle leaching method. Then, seed cells were isolated and cultured on the scaffold. Twenty-four healthy New Zealand white rabbits, 4 weeks of age, were used for the study. Under balanced anesthesia, an articular cartilage defect (4.5 mm in diameter, 5 mm in depth) was created on the rabbit’s femoral condyle using a bone drill. After modeling, rabbits were randomized into three groups and given direct suture in blank group, pure scaffold implantation in control group and implantation of the scaffold-cell complex in experimental group. Femoral condyle of each rabbit was taken out for gross and histological observations at 8, 20 weeks after surgery.
RESULTS AND CONCLUSION: At 8 weeks after surgery, transparent film-covered defects and small/irregular cells were found in the experimental group; the defects were filled with fibrous tissues in the control group; while there was no repair in the blank group. Until the 20th week, the defects were covered with hyaline cartilage-like tissues, accompanied by regular cell arrangement in the experimental group; in the control group, the defects were covered with white membranous tissues, and many chondrocytes were found at the basement and edge; in the blank group, some newborn tissues were visible at the defect region. These findings suggest that the poly (hydroxybutyrate-co- hydroxyoctanoate/collagen) osteochondral tissue-engineered scaffold carrying seed cells contributes to articular cartilage repair.

 

Key words: Cartilage, Collagen Type I, Stents, Tissue Engineering

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