中国组织工程研究

中国组织工程研究 ›› 2016, Vol. 20 ›› Issue (3): 352-358.doi: 10.3969/j.issn.2095-4344.2016.03.009

• 组织工程骨及软骨材料 tissue-engineered bone and cartilage materials • 上一篇    下一篇

富血小板纤维蛋白骨诱导动物模型的建立

肖 琼1，孙 勇2，陈红亮2，赵 峰2，董 露3，杨琴秋4，芦 帅2   

  1. 1重庆拜博口腔医院管理有限公司九龙坡口腔医院，重庆市  400000；2解放军成都军区机关医院口腔科，四川省成都市  610041；3凉山彝族自治州第一人民医院口腔科，四川省西昌市  615000；4遂宁市第一人民医院口腔科，四川省遂宁市  629000
  • 收稿日期:2015-11-07 出版日期:2016-01-15 发布日期:2016-01-15
  • 通讯作者: 孙勇，主任医师，教授，解放军成都军区机关医院口腔科，四川省成都市 610041
  • 作者简介:肖琼，女，1987年生，重庆市沙坪坝区人，汉族，2015年四川医科大学毕业，硕士，医师，主要从事口腔种植义齿修复的基础与临床研究。
  • 基金资助:
    全军“十二五”科研面上项目(CWS11J024)：新方法制备富血小板凝胶对兔颅骨骨缺损的成骨效果；军事口腔医学国家重点实验室开放课题(2014KB04)：富血小板纤维蛋白诱导骨组织再生修复的实验研究和临床观察

Construction of animal models induced by platelet-rich fibrin

Xiao Qiong1, Sun Yong2, Chen Hong-liang2, Zhao Feng2, Dong Lu3, Yang Qin-qiu4, Lu Shuai2   

  1. 1Jiulongpo Dental Hospital of Chongqing Bybo Dental Hospital Administrative Limited Company, Chongqing 400000, China; 2Department of Stomatology, the Chengdu Military Region Authorities Hospital of PLA, Chengdu 610041, Sichuan Province, China; 3Department of Stomatology, the First People’s Hospital of Liangshan Yi Autonomous Prefecture, Xichang 615000, Sichuan Province, China; 4Department of Stomatology, the First People’s Hospital of Suining, Suining 629000，Sichuan Province, China
  • Received:2015-11-07 Online:2016-01-15 Published:2016-01-15
  • Contact: Sun Yong, Chief physician, Professor, Department of Stomatology, the Chengdu Military Region Authorities Hospital of PLA, Chengdu 610041, Sichuan Province, China
  • About author:Xiao Qiong, Master, Physician, Jiulongpo Dental Hospital of Chongqing Bybo Dental Hospital Administrative Limited Company, Chongqing 400000, China
  • Supported by:

    the Military “Twelfth Five-Year” Research Program, China, No. CWS11J024; the Key Laboratory Open Project of Militay Oral Medicine, No.2014KB04

PDF

569

摘要：

文章快速阅读：

文题释义：

富血小板纤维蛋白：是一种富含细胞因子和生长因子的自体来源新型生物材料，被誉为新一代血小板浓缩物，其分子结构类似天然血凝块，为组织细胞提供迁移、增殖和分化的场所。近来，许多学者将富血小板纤维蛋白作为移植材料应用于口腔种植前上颌窦底提升术后的骨移植中，获得了良好的效果。

骨诱导性：是材料直接诱导间充质细胞分化为骨原细胞、成骨细胞，进而形成骨组织的性能。具有骨诱导性的材料即使在非骨环境中也具有激发骨生成的能力，通常通过异位植入，即非骨环境植入是否能成骨来判断一种材料是否具有骨诱导性。

 

背景：目前许多研究将富血小板纤维蛋白联合其他骨替代材料用于种植体周围骨缺损和上颌窦提升，但尚缺乏富血小板纤维蛋白单独修复临界性骨缺损方面的研究。
目的：对比富血小板纤维蛋白、Bio-Oss骨替代品、自体骨松质3种骨替代材料修复骨缺损的效果。
方法：在Beagle犬股骨髁内侧制成4处直径6.0 mm、深度10.0 mm的圆柱形临界性骨缺损，其中3处分别植入自体富血小板纤维蛋白、Bio-Oss骨替代品、自体骨松质，以不植入任何物质的为空白对照组。术后12周进行骨缺损区X射线及Micro-CT检测。
结果与结论：①X射线：富血小板纤维蛋白组密度较高，略微低于周围正常骨组织；Bio-Oss骨替代品组密度较低，但仍高于周围骨组织；自体骨松质组密度较高，接近周围骨组织；空白对照组呈明显的圆形低密度影。②Micro-CT：富血小板纤维蛋白组与自体骨松质组相似，较周围正常骨组织密度稍低，清晰可见较规律排列的骨小梁，无明显界面；Bio-Oss骨替代品组呈高密度影，界面较清；空白对照组仍然清晰可见骨缺损区，呈低密度影像。富血小板纤维蛋白组、自体骨松质组骨体积分数、骨小梁数目均高于Bio-Oss骨替代品组(P < 0.05)。表明富血小板纤维蛋白具有与自体骨相似的骨修复效果。   

关键词: 生物材料, 骨生物材料, 富血小板纤维蛋白, 骨缺损, 骨诱导, 口腔种植, 细胞因子, 微焦点计算机断层摄影系统

Abstract:

BACKGROUND: At present, many studies have focused on platelet-rich fibrin combined with other bone substitute materials in repair of peri-implant bone defects and sinus lifting, but there is still a lack of research 
about the platelet-rich fibrin alone in repair of critical-size bone defects.
OBJECTIVE: To compare the effect of three kinds of bone substitutes, platelet-rich fibrin, Bio-Oss bone substitute and autogenous cancellous bone, in repair of bone defects.
METHODS: Four areas of cylindrical critical bone defects with a diameter of 6.0 mm and depth of 10.0 mm were prepared in the medial femoral condyle of beagle dogs. Three areas of bone defects were implanted with autologous platelet-rich fibrin, Bio-Oss bone substitutes and autologous cancellous bone, respectively. The remaining one area of bone defect was not implanted any substance, as control group. X-ray and Micro-CT detections in bone defect area were conducted after 12 weeks of surgery.
RESULTS AND CONCLUSION: (1) X-ray: the density of platelet-rich fibrin group was more higher, but still slightly lower than that of the surrounding normal bone tissue; the density of the Bio-Oss bone substitute group was more lower, but still higher than that of the surrounding bone tissues; the density of autologous cancellous bone group was more higher, which was close to that of the surrounding bone tissues; circular low-density images were visible in the control group. (2) Micro-CT: platelet-rich fibrin group was similar with the autogenous cancellous bone group, and their density was slightly lower than that of the surrounding normal bone tissues; the trabecular bone exlibited a clear regular arrangement, and no obvious interface; the Bio-Oss bone substitute group showed a high density image with clear interface; the bone defect area was still clearly visible in the control group, with a low-density image. The bone volume fraction and trabecular number in the platelet-rich fibrin and autologous cancellous bone groups were both higher than those in the Bio-Oss bone substitute group (P < 0.05). These results demonstrate that platelet-rich fibrin and autogenous cancellous bone have a similar bone repair effect.