中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (46): 7435-7439.doi: 10.3969/j.issn.2095-4344.2015.46.011

• 组织构建与生物力学 tissue construction and biomechanics • 上一篇    下一篇

药物治疗骨质疏松肱骨拉伸力学特性的对比

王 强1，战祥波2，王 巍3，杨 琦4   

  1. 1吉林大学第二医院，吉林省长春市  130026；2吉化集团公司总医院，吉林省吉林市  132000；3吉林大学第一医院放射线科，吉林省长春市  130000；4吉林大学中日联谊医院妇产科，吉林省长春市  130000
  • 收稿日期:2015-09-16 出版日期:2015-11-12 发布日期:2015-11-12
  • 通讯作者: 杨琦，硕士，主治医师，吉林大学中日联谊医院妇产科，吉林省长春市 130000
  • 作者简介:王强，女，1970年生，吉林省长春市人，汉族，1996年吉林大学毕业，博士，副主任医师，主要从事女性卵巢功能与激素水平对其他组织功能的影响的研究。

Drug therapies for osteoporosis: tensile properties of the humerus

Wang Qiang1, Zhan Xiang-bo2, Wang Wei3, Yang Qi4   

  1. 1the Second Hospital of Jilin University, Changchun 130026, Jilin Province, China; 2General Hospital of Jilin Chemical Group Corporation, Jilin 132000, Jilin Province, China; 3Department of Radiology, First Bethune Hospital of Jilin University, Changchun 130000, Jilin Province, China; 4Department of Obstetrics and Gynecology, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin Province, Chi
  • Received:2015-09-16 Online:2015-11-12 Published:2015-11-12
  • Contact: Yang Qi, Master, Attending physician, Department of Obstetrics and Gynecology, China-Japan Union Hospital of Jilin University, Changchun 130000, Jilin Province, China
  • About author:Wang Qiang, M.D., Associate chief physician, the Second Hospital of Jilin University, Changchun 130026, Jilin Province, China

PDF

292

被引次数

4

摘要：

背景：力学性能指标是评价药物治疗骨质疏松动物模型效果的重要方法，以拉伸力学性能指标评价多种药物治疗老龄雌性骨质疏松模型大鼠的效果评价罕见报道。
目的：评定中药骨疏康胶囊、西药阿仑膦酸钠、维生素K、钙剂逸得乐对骨质疏松模型大鼠肱骨拉伸力学的影响。
方法：四五个月龄雌性Wistar大鼠180只分为6组，每组30只。除正常对照组外对所有大鼠于0周摘除双侧卵巢制备骨质疏松动物模型。骨疏康胶囊组动物每日给服骨疏康胶囊0.9 g/kg、阿仑膦酸钠治疗组动物每日给服阿仑膦酸钠1 mg/kg、维生素K组动物每日给服维生素K 0.1 mg/kg、钙剂组动物每日给服钙剂逸得乐2 mg/kg。15周后取大鼠左、右侧肱骨在电子万能试验机上以5 mm/min的实验速度进行拉伸实验，试样破坏后观察试样的断口形貌。
结果与结论：骨质疏松动物模型组大鼠肱骨拉伸实验最大载荷、最大应变显著小于正常对照组和骨疏康胶囊组、阿仑膦酸钠组、维生素K组(P < 0.05)；钙剂组最大载荷，最大应力，最大应变和模型组差异不显著(P > 0.05)。结果说明，中药、西药、维生素K治疗骨质疏松模型大鼠拉伸力学性能均有一定提高，中药骨疏康胶囊治疗骨质疏松模型大鼠拉伸力学性能明显提高。
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 组织构建, 骨组织工程, 骨质疏松, 动物模型, 药物治疗, 力学实验

Abstract:

BACKGROUND: Mechanical property test is an important method to evaluate the effect of drug treatment on the animal model of osteoporosis. But there are rare reports on the tensile mechanical test for evaluating the therapeutic effects of a variety of drugs on a female rat model of senile osteoporosis.
OBJECTIVE: To evaluate the effects of Gusukang capsule, alendronate, vitamin K and calcium on tensile properties of the humerus in the rat model of osteoporosis.
METHODS: Totally 180 female Wistar rats, 4-5 months old, were randomized into six groups, 30 in each group. Except the rats in the normal control group, all the rats were subjected to make ovariectomized osteoporosis models. Then, rats were given Gusukang capsule (0.9 g/kg/d), alendronate (1 mg/kg/d), vitamin K (0.1 mg/kg/d) and calcium (2 mg/kg/d) in the Gusukang capsule, alendronate, vitamin K and calcium groups, respectively. After 15 weeks, the bilateral humeri were taken from all the rats, and the tensile test was carried out on an electronic universal testing machine at a speed of 5 mm/min. The fracture morphology of the specimen was observed.
RESULTS AND CONCLUSION: The maximum load and maximum strain of the rat fumerus were significantly 
lower in the model group than in the normal control, Gusukang capsule, alendronate and vitamin K groups (P < 0.05). There were no differences in the maximum load, maximum stress and maximum strain between the calcium group and model group (P > 0.05). These findings indicate that the tensile properties of the humerus can be improved by Chinese medicine, western medicine and vitamin K, especially by the Chinese medicine. 
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoporosis, Models, Animal, Drugs, Chinese Herbal, Biomechanics, Tissue Engineering