中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (46): 7406-7412.doi: 10.3969/j.issn.2095-4344.2015.46.006

• 口腔组织构建 oral tissue construction • 上一篇    下一篇

正畸牙移动过程中尼古丁摄入对牙周组织影响

潘 旭1，2，祖丽呼玛•阿热甫江1，胡明华1，2，聂 晶1，2，米丛波1，2，杨凤莲3   

  1. 1新疆医科大学第一附属医院口腔正畸科，新疆维吾尔自治区乌鲁木齐市  830054；2新疆医科大学口腔医学院口腔正畸教研室，新疆维吾尔自治区乌鲁木齐市  830054；3新疆库尔勒市农二师医院综合门诊部口腔科，新疆维吾尔自治区库尔勒市  841000
  • 收稿日期:2015-08-29 出版日期:2015-11-12 发布日期:2015-11-12
  • 通讯作者: 米丛波，新疆医科大学第一附属医院口腔正畸科，新疆维吾尔自治区乌鲁木齐市 830054；新疆医科大学口腔医学院口腔正畸教研室，新疆维吾尔自治区乌鲁木齐市 830054
  • 作者简介:潘旭，女，1983年生，山东省平度市人，汉族，2010年新疆医科大学毕业，硕士，主治医师，主要从事错牙合畸形防治的研究。 并列第一作者：祖丽呼玛?阿热甫江，女，1986年生，新疆维吾尔自治区伊宁县人，维吾尔族，2013年新疆医科大学毕业，硕士，医师，主要从事错牙合畸形防治的的研究。
  • 基金资助:

    新疆医学动物模型研究实验室开放课题基金资助(XJDX1103-2012-04)，课题名称：尼古丁对正畸力作用下大鼠牙周组织改建的影响

Effect of nicotine intake on the periodontal tissue during orthodontic tooth movement

Pan Xu1, 2, Zulihuma Arefujiang1, Hu Ming-hua1, 2, Nie Jing1, 2, Mi Cong-bo1, 2, Yang Feng-lian3   

  1. 1Department of Orthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; 2Department of Orthodontics, School of Stomatology, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; 3Department of Stomatology, General Clinic of Agricultural Second Division, Korla 841000, Xinjiang Uygur Autonomous Region, China
  • Received:2015-08-29 Online:2015-11-12 Published:2015-11-12
  • Contact: Mi Cong-bo, Department of Orthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Department of Orthodontics, School of Stomatology, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
  • About author:Pan Xu, Master, Attending physician, Department of Orthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China; Department of Orthodontics, School of Stomatology, Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China Zulihuma Arefujiang, Master, Physician, Department of Orthodontics, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China Pan Xu and Zulihuma Arefujiang contributed equally to this work.
  • Supported by:

    the Open Project of Xinjiang Medical Animal Model Laboratory, No. XJDX1103-2012-04

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293

被引次数

1

摘要：

背景：吸烟是严重影响牙周组织和牙根健康的危险因素之一，烟草中的尼古丁会加速牙周病患者牙周组织的破坏。
目的：分析正畸牙移动过程中不同剂量尼古丁作用下牙周组织内环氧化酶2及mRNA表达的变化规律。
方法：将110只SD大鼠随机分为5组，空白对照组10只，生理盐水组和尼古丁0.5，0.75，1 mg/kg组各25只。除空白组外所有大鼠使用50 g力牵拉一侧上颌第1磨牙向近中移动。生理盐水组每日腹腔注射0.1 mL生理盐水；尼古丁组每日腹腔注射0.5，0.75，1 mg/kg尼古丁酒石酸溶液。各组大鼠分别在加力1，3，5，7，14 d时处死取上颌组织，苏木精-伊红染色观察牙周组织变化，免疫组织化学染色计数破牙骨质细胞阳性细胞，原位杂交染色法检测环氧化酶2在牙周组织中表达的平均吸光度值。
结果与结论：尼古丁各剂量组在各加力时间点破牙骨质细胞数目均高于未注射尼古丁组，且环氧化酶2阳性细胞表达强度也均高于未注射尼古丁组。随尼古丁注射剂量增大，破牙骨质细胞数目也逐渐增加(P < 0.05)，加力7 d时各组破牙骨质细胞数目均达到峰值；环氧化酶2阳性细胞表达强度也随尼古丁注射剂量增大而增强(P < 0.05)，加力5 d时表达强度达到峰值。结果表明，在相同加力时间点，破牙骨质细胞数目随剂量增大而增多，且环氧化酶2阳性细胞表达强度也随剂量增加而增强。正畸牙移动过程中的尼古丁摄入会造成牙周组织的破环，且尼古丁摄入剂量的高低直接影响牙周组织的破坏程度。
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

关键词: 组织构建, 组织工程, 尼古丁, 尼古丁酒石酸溶液, 正畸牙移动, 免疫组织化学, 原位杂交, 环氧化酶2, 环氧化酶2 mRNA, 破牙骨质细胞, 牙根吸收, 牙周组织

Abstract:

BACKGROUND: Cigarette smoking can seriously damage the periodontal tissues and root, and the nicotine in tobacco accelerates the progression of periodontal diseases.
OBJECTIVE: To investigate the effect of different doses of nicotine on the expression of cyclooxygenase-2 mRNA in the periodontal tissue during orthodontic tooth movement.
METHODS: Totally 110 Sprague-Dawley rats were randomly divided into blank control group (n=10), normal saline group (n=25), 0.5 mg/kg nicotine group (n=25), 0.75 mg/kg nicotine group (n=25), 1 mg/kg nicotine group (n=25). Rats in the normal saline groups were injected intraperitoneally with 0.1 mL normal saline, and those in the three nicotine groups were respectively injected with 0.5, 0.75, 1 mg/kg nicotine tartrate solution. Except the blank control group, the unilateral maxillary first molars of rats in the other four groups were exposed to 50 g force. At 1, 3, 5, 7, 14 days under force, the rats were sacrificed to take the maxillary tissues. Hematoxylin-eosin staining was used to observe the changes of periodontal tissues, immunohistochemical staining was employed to count positive cells, and in situ hybridization staining was adopted to detect the mean absorbance value of cyclooxygenase-2 in the periodontal tissues.
RESULTTS AND CONCLUSION: The number of odontoclasts and the expression of cyclooxygenase-2 in the nicotine groups were higher than those in the non-nicotine groups. With the increasing dose of nicotine, the number of odontoclasts gradually increased, and the difference was statistically significant (P < 0.05). At 7 days under force, the number of odontoclasts reached the peak. With the increasing dose of nicotine, the positive expression intensity of cyclooxygenase-2 was also increased, and the difference was statistically significant (P < 0.05). The expression of cyclooxygenase-2 reached peak at 5 days under force. These findings indicate that with the increasing dose of nicotine, the number of odontoclasts and the expression intensity of cyclooxygenase-2 are both increased at the same time point under force. During the orthodontic tooth movement, the intake of nicotine can damage the periodontal tissue, and the dose of nicotine can directly influence the severity of damage to the periodontal tissue. 
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Nicotine, Orthodontics, Cyclooxygenase 2, Dental Cementum