中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (27): 4365-4370.doi: 10.3969/j.issn.2095-4344.2015.27.019

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

口腔鳞状细胞癌及癌旁正常组织基因表达谱芯片检测

郑建伟1，2，杨淑娟3，利小平2，韦从云2，李  婷2，莫文娟2，蔡秋云2，杨德群2，周  磊1，罗  刚1   

  1. 1南方医科大学附属广东省口腔医院，广东省广州市  510280；
    2广东省深圳牙科医疗中心，广东省深圳市  518001；
    3四川大学华西公共卫生学院健康与社会行为学系，四川省成都市  610041
  • 出版日期:2015-06-30 发布日期:2015-06-30
  • 通讯作者: 罗刚，博士，主任医师，南方医科大学附属广东省口腔医院，广东省广州市 510280 周磊，博士，主任医师，南方医科大学附属广东省口腔医院，广东省广州市 510280
  • 作者简介:郑建伟，男，1981年生，汉族，四川省成都市人，南方医科大学在读博士，主治医师，主要从事口腔医学方面的研究。
  • 基金资助:

    广东省医学科学研究基金资助项目(A2011092)；国家自然科学基金资助项目(81271159/H1405)

DNA chip-based gene expression profiling of oral squamous cell carcinoma tissue and normal paracarcinoma tissue

Zheng Jian-wei1, 2, Yang Shu-juan3, Li Xiao-ping2, Wei Cong-yun2, Li Ting2, Mo Wen-juan2, Cai Qiu-yun2, Yang De-qun2, Zhou Lei1, Luo Gang1   

  1. 1Affiliated Stomatology Hospital of Guangdong Province, South Medical University, Guangzhou 510280, Guangdong Province, China; 
    2Shenzhen Dental Medical Center of Guangdong Province, Shenzhen 518001, Guangdong Province, China; 
    3Department of Public Health and Social and Behavioral Sciences, West China School of Public Health, Sichuan University, Chengdu 610041, Sichuan Province, China
  • Online:2015-06-30 Published:2015-06-30
  • Contact: Corresponding author: Luo Gang, M.D., Chief physician, Affiliated Stomatology Hospital of Guangdong Province, South Medical University, Guangzhou 510280, Guangdong Province, China Corresponding author: Zhou Lei, M.D., Chief physician, Affiliated Stomatology Hospital of Guangdong Province, South Medical University, Guangzhou 510280, Guangdong Province, China
  • About author:Zheng Jian-wei, Studying for doctorate, Attending physician, Affiliated Stomatology Hospital of Guangdong Province, South Medical University, Guangzhou 510280, Guangdong Province, China; Shenzhen Dental Medical Center of Guangdong Province, Shenzhen 518001, Guangdong Province, China
  • Supported by:

    Medical Science Research Foundation of Guangdong Province of China, No. A2011092; the National Natural Science Foundation of China, No. 81271159\H1405

PDF

599

被引次数

5

摘要：

背景：近年在整体水平上以高通量分子扫描手段为基础的基因组学、蛋白质组学以及计算机辅助设计等技术的整合及相互关联的“技术链”的应用已在乳腺癌、肺癌、胃癌、结肠癌、卵巢癌、黑色素瘤等的研究中取得了丰硕的成果，但关于口腔鳞状细胞癌的研究较少。
目的：实验通过基因表达谱芯片检测口腔鳞状细胞癌组织与癌旁正常组织的基因表达谱。
方法：收集广东省口腔医院2013年手术切除的口腔鳞癌及癌旁正常组织各2例，采用Roche NimbleGen全基因组表达谱芯片进行口腔鳞癌及癌旁正常组织的基因表达谱检测。
结果与结论：按差异基因筛选标准，从32 448条检测基因中筛选出口腔鳞癌肿瘤组织的差异基因共有7 872条，占筛选基因总数的24%；其中上调表达的基因有3 800条，下调表达的有4 072条。结果证实，通过基因表达谱芯片检测并根据表达差异1倍以上的筛选标准得到了7 872个表达差异的基因。由此可见肿瘤的发生发展不是单个或几个基因的作用结果，以往实验往往针对某个或某几个基因的研究有很大的局限性。同时也说明了肿瘤的产生是多基因成网络相互调节作用的结果，而且这个网络的作用关系是非常复杂的。

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

关键词: 实验动物, 组织工程, 口腔鳞状细胞癌, 基因芯片, 基因表达谱芯片, Roche NimbleGen全基因组表达谱芯片, 差异基因, 生物信息学分析, DNA, 质量评价, 国家自然科学基金

Abstract:

BACKGROUND: In recent years, based on high-throughput molecular imaging, integration of genomics, proteomics and computer aided design and the application of correlative “technical chains” have achieved great achievements in the research of breast cancer, lung cancer, gastric cancer, colon cancer, ovarian cancer and melanin tumor. However, there are few researches on oral squamous cell carcinoma.
OBJECTIVE: To detect the gene expression profile of the oral squamous cell carcinoma tissue and normal paracarcinoma tissue using DNA chip-based gene expression profile.
METHODS: Two samples of oral squamous cell carcinoma tissue and normal paracarcinoma tissue of patients who received treatment at Stomatological Hospital of Guangdong Province of China in 2013 were included in this study. The gene expression profiles of oral squamous cell carcinoma and normal paracarcinoma tissue were determined by the Roche NimbleGen gene expression microarrays.
 
RESULTS AND CONCLUSION: According to screening criteria of differential genes, 7 872 out of 32 448 detected genes were differentially expressed genes of oral squamous cell carcinoma, which accounts for 24% of the total number of the screening genes. 3 800 genes were up-regulated, and 4 072 were down-regulated. The results confirm that through detection with the help of gene expression profile clip, 7 872 differentially expressed genes were obtained through DNA chip-based gene expression profiles according to the screening criteria. Thus it can be concluded that the occurrence and development of the tumors are not a result of single or several genes. Previous experiments based on a single or several genes have great limitations. These findings also suggest that the occurrence of tumor is a result of mutual regulatory effects of many genes forming a network, moreover, the interactions of the network is quite complicated.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Microchip Analytical Procedures, Oral Spray, Tumor

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