中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (24): 3808-3812.doi: 10.3969/j.issn.2095-4344.2015.24.008

• 组织构建与生物活性因子 tissue construction and bioactive factors • 上一篇    下一篇

细胞因子抑制剂吡非尼酮对体外培养人瘢痕成纤维细胞增殖的影响

蓝  蔚1，李孝建2，纪雪亮1，易先锋1，刘衍智1，涂荣梅1   

  1. 1广东省工伤康复医院烧伤康复科，广东省广州市  510440；
    2暨南大学第一临床医学院，广州市红十字会医院烧伤整形科，广东省广州市  510220
  • 出版日期:2015-06-11 发布日期:2015-06-11
  • 通讯作者: 李孝建，硕士，主任医师，暨南大学第一临床医学院，广州市红十字会医院烧伤整形科，广东省广州市 510220
  • 作者简介:蓝蔚，男，1979年生，畲族，江西省人，2009年南昌大学毕业，硕士，副主任医师。
  • 基金资助:

    广东省医学科学技术研究基金项目(B2012300)

Pirfenidone effects on human hypertrophic scar fibroblasts cultured in vitro 

Lan Wei1, Li Xiao-jian2, Ji Xue-liang1, Yi Xian-feng1, Liu Yan-zhi1, Tu Rong-mei1   

  1. 1Department of Burn Rehabilitation, Guangdong Provincial Work Injury Rehabilitation Hospital, Guangzhou 510440, Guangdong Province, China; 
    2First Clinical Medical College of Jinan University, Department of Burns and Plastic Surgery, Guangzhou Red Cross Hospital, Guangzhou 510220, Guangdong Province, China
  • Online:2015-06-11 Published:2015-06-11
  • Contact: Li Xiao-jian, Master, Chief physician, First Clinical Medical College of Jinan University, Department of Burns and Plastic Surgery, Guangzhou Red Cross Hospital, Guangzhou 510220, Guangdong Province, China
  • About author:Lan Wei, Master, Associate chief physician, Department of Burn Rehabilitation, Guangdong Provincial Work Injury Rehabilitation Hospital, Guangzhou 510440, Guangdong Province, China
  • Supported by:

    the Guangdong Medical Science and Technology Research Foundation, No. B2012300

PDF

258

被引次数

3

摘要：

背景：有研究表明细胞因子抑制剂吡非尼酮通过调控多种细胞因子，抑制成纤维细胞的生物学活性，其在内脏器官的抗纤维化作用的研究和应用取得了良好的进展，但对于皮肤增生性瘢痕及成纤维细胞是否有影响及其机制尚不清楚。
目的：观察细胞因子抑制剂吡非尼酮对人增生性瘢痕成纤维细胞增殖的影响。
方法：采用组织块法培养人增生性瘢痕成纤维细胞，实验取第3-6代生长状态良好的对数生长期细胞。根据吡非尼酮不同质量浓度分为对照组(吡非尼酮0 g/L)，吡非尼酮0.15，0.3，1 g/L组，共干预12，36，48 h。
结果与结论：MTT，反转录-聚合酶链式反应和酶链免疫吸附实验结果显示，与对照组相比，吡非尼酮0.15，0.3，1 g/L组细胞增殖情况、转化生长因子β1 mRNA的表达、Ⅰ、Ⅲ型胶原蛋白的分泌量均降低(P < 0.05)，其中吡非尼酮1 g/L组降低最明显(P < 0.05)。干预24，48，72 h后，吡非尼酮0.15，0.3，1 g/L组间细胞增殖抑制率、Ⅰ、Ⅲ型胶原蛋白的分泌量均差异有显著性意义(P < 0.05)。结果证实，细胞因子抑制剂吡非尼酮对体外培养的人增生性瘢痕成纤维细胞胶原蛋白的分泌、转化生长因子β1的表达及细胞增殖活性有明显的抑制作用。


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

关键词: 组织构建, 成纤维细胞, 细胞因子抑制剂, 吡非尼酮, 增生性瘢痕, 细胞培养, Ⅰ胶原蛋白, Ⅲ胶原蛋白, 组织块法, 4-甲偶氮唑蓝法, 酶链免疫吸附法, 反转录-聚合酶链反应

Abstract:

BACKGROUND: Studies have shown that cytokine inhibitor pirfenidone can inhibit biological activity of fibroblasts by regulating a variety of cytokines. It has made good progress in the research and application of anti-fibrosis of internal organs, but the effect and mechanism for hypertrophic scars and skin fibroblasts are unclear.
OBJECTIVE: To investigate the effect of pirfenidone on human hypertrophic scar fibroblasts.
METHODS: Human hypertrophic scar fibroblasts were cultured using tissue culture method. Passages 3-6 cells grew well in the logarithmic growth phase were collected. Cells were divided into the control group (0 g/L pirfenidone), 0.15, 0.3 and 1 g/L pirfenidone groups according to different mass concentrations. Cells were intervened for 12, 36 and 48 hours.
RESULTS AND CONCLUSION: MTT, reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay results demonstrated that compared with the control group, cell proliferation, transforming growth factor β1 mRNA expression, types I and III collagen secretion were decreased in the 0.15, 0.3 and 1 g/L pirfenidone groups (P < 0.05), and the decrease was most significant in the 1 g/L pirfenidone group (P < 0.05). At 24, 48 and 72 hours after intervention, significant differences in inhibitory rate of cell proliferation and the  
secretion of types I and III collagen were detected among 0.15, 0.3 and 1 g/L pirfenidone groups (P < 0.05). Results confirmed that pirfenidone apparently inhibited the secretion of collagen of hypertrophic scar fibroblasts cultured in vitro, transforming growth factor β1 expression and cell proliferation and viability. 


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Cytokines, Transforming Growth Factor beta1, Cell Proliferation, Cicatrix, Fibroblasts

中图分类号: