中国组织工程研究

中国组织工程研究 ›› 2015, Vol. 19 ›› Issue (8): 1160-1164.doi: 10.3969/j.issn.2095-4344.2015.08.003

• 纳米生物材料 nanobiomaterials • 上一篇    下一篇

纳米羟基磷灰石/壳聚糖/半水硫酸钙为可注射骨组织工程支架材料的可行性

薛 震1，牛丽媛2，安 刚3，郭亚山3，吕松岑1   

  1. 哈尔滨医科大学附属第二医院，1骨科四病房，3急诊创伤病房，黑龙江省哈尔滨市 150086；2哈尔滨工业大学校医院，黑龙江省哈尔滨市 150086
  • 修回日期:2015-01-15 出版日期:2015-02-19 发布日期:2015-02-19
  • 通讯作者: 薛震，哈尔滨医科大学附属第二医院骨科四病房，黑龙江省哈尔滨市 150086
  • 作者简介:薛震，男，1976年生，辽宁省兴城市人，汉族，2004年哈尔滨医科大学毕业，硕士，主治医师，主要从事骨组织工程方面的研究。
  • 基金资助:

    黑龙江省教育厅科学技术研究项目(12511202)

Feasibility of nano-hydroxyapatite/chitosan/calcium sulphate hemihydrate as an injectable bone tissue engineering scaffold  

Xue Zhen1, Niu Li-yuan2, An Gang3, Guo Ya-shan3, Lv Song-cen1   

  1. 1The Fourth Ward, Department of Orthopedic Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China; 2Affiliated Hospital of Harbin Institute of Technology, Harbin 150086, Heilongjiang Province, China; 3Department of Emergency Trauma, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • Revised:2015-01-15 Online:2015-02-19 Published:2015-02-19
  • Contact: Xue Zhen, the Fourth Ward, Department of Orthopedic Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • About author:Xue Zhen, Master, Attending physician, the Fourth Ward, Department of Orthopedic Surgery, Second Affiliated Hospital of Harbin Medical University, Harbin 150086, Heilongjiang Province, China
  • Supported by:

    the Science and Technology Research Project of Heilongjiang Provincial Education Department, No. 12511202

     

PDF

688

被引次数

20

摘要：

背景：前期实验采用仿生学原理制备了可注射性纳米羟基磷灰石/壳聚糖/半水硫酸钙复合材料，但其与骨髓间充质干细胞的生物相容性还不十分清楚。

目的：探讨纳米羟基磷灰石/壳聚糖/半水硫酸钙作为注射型骨组织工程支架材料的可行性。
方法：将第3代兔骨髓间充质干细胞与可注射纳米羟基磷灰石/壳聚糖/半水硫酸钙支架复合培养，作为实验组；以单纯接种培养的骨髓间充质干细胞为对照组，倒置显微镜下观察细胞生长情况，MTT法检测细胞增殖，扫描电镜观察细胞在材料表面生长与增殖。将纳米羟基磷灰石/壳聚糖/半水硫酸钙支架埋植在家兔背部肌袋内，埋植后2，4，6，8周进行病理学观察。
结果与结论：实验组细胞生长、增殖良好，与对照组无明显差异。支架埋植后2周，材料周围有中等量中性粒细胞、淋巴细胞和巨细胞浸润，可见小血管与纤维母细胞增生，材料已被炎性细胞分割、围绕散碎；埋植后4周，可见少量淋巴细胞、纤维母细胞聚集，炎症反应进一步消退，肌纤维排列、形态正常；埋植后6周，材料周围炎症反应轻微，组织水肿不明显；埋植后8周，炎症反应基本消退，材料基本降解完成，肌纤维形态基本正常。表明纳米羟基磷灰石/壳聚糖/半水硫酸钙复合物具有良好的细胞相容性和生物降解性，可作为注射型支架材料。

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

关键词: 生物材料, 骨生物材料, 半水硫酸钙, 组织工程, 羟基磷灰石, 壳聚糖, 生物相容性, 骨髓间充质干细胞

Abstract:

BACKGROUND: In preliminary experiments, injectable nano-hydroxyapatite/chitosan/calcium sulphate hemihydrates (nHA/CS/CSH) composites have been developed based on the bionics principle, but the biocompatibility of this composite with bone marrow mesenchymal stem cells is not very clear.

OBJECTIVE: To study the feasibility of nHA/CS/CSH as an injectable bone tissue engineering scaffold.
METHODS: Bone marrow mesenchymal stem cells from rabbits were cultured and induced, and the third passage cells were cultured with nHA/CS/CSH as an experimental group. Bone marrow mesenchymal stem cells subjected to inoculated culture were used as controls. The morphology and growth of the cells were observed under inverted phase contrast microscope. MTT assay was used to examine cell proliferation. The morphology and proliferation of the cells on the surface of nHA/CS/CSH was observed by scanning electron microscope. The nHA/CS/CSH scaffold was implanted into the rabbit sacrospinal muscles, and pathologically observed after 2, 4, 6, 8 weeks.

RESULTS AND CONCLUSION: Bone marrow mesenchymal stem cells co-cultured with nHA/CS/CSH exhibited good growth as observed under inverted phase contrast microscope, without significant difference from those in the control group. At 2 weeks after scaffold implantation, a moderate amount of neutrophils, lymphocytes and giant cells infiltrated around the graft material, small blood vessels and fibroblasts proliferated, and the graft material was divided and embraced by inflammatory cells; at 4 weeks after implantation, a few lymphocytes, fibroblasts aggregated, the inflammation subsided further, and muscle fibers showed normal arrangement and morphology; at 6 weeks after implantation, the inflammation around the graft material became mild and tissue edema was not obvious; at 8 weeks after implantation, the inflammation subsided basically, the material degradation was complete, and the muscle fibers had normal morphology. These findings indicate that the nHA/CS/CSH composite material has good biocompatibility and biodegradation, which may be used as a good scaffold material for injectable bone tissue engineering scaffold.


中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程


全文链接：

Key words: Tissue Engineering, Hydroxyapatites, Chitosan

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