中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (36): 6417-6422.doi: 10.3969/j.issn.2095-4344.2013.36.006

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

心肌细胞培养上清诱导骨髓间充质干细胞的分化

李春梅1，王秀力1，朱海亮2，王  洁1，宫海滨1   

  1. 1徐州市心血管病研究所，徐州市中心医院，江苏省徐州市  221009；2南京大学生命科学学院，江苏省南京市  210093
  • 收稿日期:2013-01-10 修回日期:2013-02-02 出版日期:2013-09-03 发布日期:2013-09-03
  • 通讯作者: 宫海滨，博士，教授，博士生导师，徐州市心血管病研究所，徐州市中心医院，江苏省徐州市 221009 ghbxzh@yahoo.com.cn
  • 作者简介:李春梅，女，1963年生，山东省青岛市人，汉族，2009年江汉大学毕业，助理研究员，主要从事干细胞的相关研究。 xzheart@sohu.com
  • 基金资助:

    国家自然科学基金资助项目(30572073)*；江苏省自然科学基金项目(DK2005428)*；江苏省卫生厅重大项目(K200402)*；江苏省医学重点人才(RC2007024)*；徐州市科技发展项目(XF10C029)*

Supernatant of myocardiocyte induces differentiation of bone marrow-derived mesenchymal stem cells

Li Chun-mei1, Wang Xiu-li1, Zhu Hai-liang2, Wang Jie1, Gong Hai-bin1   

  1. 1Xuzhou Cardiovascular Disease Institute, Xuzhou Central Hospital, Xuzhou  221009, Jiangsu Province, China; 2Life Science College of Nanjing University, Nanjing  210093, Jiangsu Province, China
  • Received:2013-01-10 Revised:2013-02-02 Online:2013-09-03 Published:2013-09-03
  • Contact: Gong Hai-bin, M.D., Professor, Doctoral supervisor, Xuzhou Cardiovascular Disease Institute, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu Province, China ghbxzh@yahoo.com.cn
  • About author:Li Chun-mei, Assistant investigator, Xuzhou Cardiovascular Disease Institute, Xuzhou Central Hospital, Xuzhou 221009, Jiangsu Province, China xzheart@sohu.com
  • Supported by:

    the National Natural Science Foundation of China, No. 30572073*; the Natural Science Foundation of Jiangsu Province, No. DK2005428*; the Key Program of Department of Health of Jiangsu Province, No. K200402*; the Key Medical Scholars of Jiangsu Province, No. RC2007024*; the Science and Technology Development Program of Xuzhou, No. XF10C029*

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423

被引次数

7

摘要：

背景：前期研究发现，心肌细胞培养上清具有诱导骨髓间充质干细胞分化为心肌样细胞的能力，这可能与培养上清内的某种细胞因子或几种细胞因子有关。
目的：分析大鼠心肌细胞培养上清诱导骨髓间充质干细胞分化为心肌样细胞是否与心肌细胞培养上清内细胞因子含量不同有关。
方法：采用全骨髓贴壁培养法分离骨髓间充质干细胞并在体外培养纯化，酶消化法分离心肌细胞，分别以 1×108 L-1的细胞浓度培养72 h后收集上清。用酶联免疫吸附试验技术检测心肌细胞和骨髓间充质干细胞培养上清内的肝细胞生长因子、胰岛素样生长因子1、血小板源生长因子、干细胞因子、成纤维细胞生长因子和血管内皮生长因子的水平。
结果与结论：心肌细胞培养上清组内的胰岛素样生长因子1、血小板源生长因子和成纤维细胞生长因子水平明显高于骨髓间充质干细胞培养上清组(P < 0.01)，提示心肌细胞培养上清内的胰岛素样生长因子1、血小板源生长因子和成纤维细胞生长因子可能与诱导骨髓间充质干细胞分化为心肌样细胞有关，其中主要的细胞因子是胰岛素样生长因子1。

关键词: 干细胞, 间质干细胞, 肌细胞, 心脏, 胰岛素样生长因子Ⅰ, 血小板源性生长因子, 肝细胞生长因子

Abstract:

BACKGROUND: Culture supernatant containing myocardiocyte has been demonstrated to induce differentiation of bone marrow-derived mesenchymal stem cells into myocardiocyte-like cells. This may associate with some or several cytokines in the culture supernatant.
OBJECTIVE: To explore if the supernatant of myocardiocyte induces bone marrow-derived mesenchymal stem cells to differentiate into myocardiocyte-like cells is associated with the different cytokine content in the supernatant of myocardiocyte.
METHODS: Bone marrow-derived mesenchymal stem cells were isolated and cultured in vitro by the whole bone marrow adherent culture. Cardiocytes were isolated and cultured by enzyme digestion. Bone marrow-derived mesenchymal stem cells (1×108/L) and cardiocytes (1×105/L) were cultured for 72 hours and the supernatant was collected. Hepatocyte growth factor, insulin-like growth factor 1, platelet-derived growth factor, stem cell factor, fibroblast growth factor and vascular endothelial growth factor levels in culture supernatant of bone marrow-derived mesenchymal stem cells and cardiocytes were detected by enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: The content of insulin-like growth factor 1, platelet-derived growth factor and fibroblast growth factor in the supernatant of cardiocytes was significantly higher in cardiocytes group compared with bone marrow-derived mesenchymal stem cells group (P < 0.01). Results indicated that insulin-like growth factor 1, platelet-derived growth factor and fibroblast growth factor in the supernatant of cardiocytes may have capability to induce bone marrow-derived mesenchymal stem cells to differentiate into myocardiocyte-like cells, and insulin-like growth factor 1 may serve as the main cytokine.

Key words: stem cells, mesenchymal stem cells, myocytes, cardiac, insulin-like growth factor I, platelet-derived growth factor, hepatocyte growth factor

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