中国组织工程研究

• 干细胞学术探讨 stem cell academic discussion • 上一篇    下一篇

诱导性多潜能干细胞的安全性及临床应用

张  杨,李秀兰   

  1. 天津医院细胞工程室,天津市  300211
  • 收稿日期:2013-03-21 修回日期:2013-05-04 出版日期:2013-07-02 发布日期:2013-07-02
  • 通讯作者: 李秀兰,研究员,博士生导师,天津医院细胞工程室,天津市 300211 lixiulan1954@sina.com
  • 作者简介:张杨★,女,1972年生,河南省信阳市人,汉族,2004年天津医科大学毕业,硕士,副研究员,主要从事创伤修复及组织工程的研究。
  • 基金资助:

    天津市应用基础及前沿技术研究计划项目,11JCYBJC10200。课题名称:诱导成纤维细胞经ips细胞途径形成皮肤和骨作用的研究。

Safety and clinical application of induced pluripotent stem cells

Zhang Yang, Li Xiu-lan   

  1. Cell Engineering Laboratory of Tianjin Orthopaedic Hospital, Tianjin  300211, China
  • Received:2013-03-21 Revised:2013-05-04 Online:2013-07-02 Published:2013-07-02
  • Contact: Li Xiu-lan, Master, Investigator, Doctoral supervisor, Cell Engineering Laboratory of Tianjin Orthopaedic Hospital, Tianjin 300211, China lixiulan1954@sina
  • About author:comZhang Yang★, Master, Associate investigator, Cell Engineering Laboratory of Tianjin Orthopaedic Hospital, Tianjin 300211, China
  • Supported by:

    Tianjin Applied Basis and Cutting-Edge Technology Research Plan, No. 11JCYBJC10200*

摘要:

背景:诱导性多潜能干细胞由体细胞重编程获得,有着巨大的潜在的临床应用价值,安全性问题备受关注。
目的:综述诱导性多潜能干细胞的安全性研究与临床应用现状。
方法:由第一作者计算机检索2006/2012年PubMed 数据库中有关诱导性多潜能干细胞诱导方法安全性研究及临床应用价值的相关文献。初检得到文献203篇,最终入选47篇文献进行综述。
结果与结论:目前提高诱导性多潜能干细胞安全性的措施主要包括:避免c-Myc基因的使用,以其它介导方式代替反转录病毒的使用,重编程因子蛋白的直接导入,使用安全性较高的供体细胞,小分子化合物及其它非转基因方式的应用等。诱导性多潜能干细胞既有广阔的临床治疗前景,还可用于疾病特异性诱导性多潜能干细胞系的建立。

关键词: 干细胞, 干细胞学术探讨, 诱导性多潜能干细胞, 安全性, 重编程, 基因, 病毒, 小分子化合物, 临床应用, 疾病, 省级基金

Abstract:

BACKGROUND: Induced pluripotent stem cells are obtained from somatic cells by reprogramming method. The safety of induced pluripotent stem cells has attracted much attention because of their huge and potential value in clinical application.
OBJECTIVE: To review the current studies addressing the safety and clinical application of induced pluripotent stem cells.
METHODS: The PubMed database between 2006 and 2012 was retrieved by the first author to search the correlative documents concerning the safety and clinical application of induced pluripotent stem cells. Totally 203 papers were primarily gotten. Finally, 47 papers were included.
RESULTS AND CONCLUSION: At present, the main methods to enhance the safety of induced pluripotent stem cells include avoiding usage of c-Myc gene, another mediate way replacing the retrovirus, direct leading of reprogramming factor protein, safer donor cells, micromolecule compound and other in-transgenosis ways. Induced pluripotent stem cells have extensive clinic treatment prospects, and can be used for the build of disease-specific induced pluripotent stem cells line.

Key words: stem cells, stem cell academic discussion, induced pluripotent stem cells, safety, reprogramming, gene, virus, small molecular compound, clinical application, diseases, provincial grants-supported paper

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