中国组织工程研究

中国组织工程研究 ›› 2013, Vol. 17 ›› Issue (2): 191-195.doi: 10.3969/j.issn.2095-4344.2013.02.001

• 组织构建基础实验 basic experiments in tissue construction •    下一篇

三种方法制备组织工程气管支架的比较

吴 凡1，张志培1，汪 健1，李建忠1，金 岩2，姜 涛1，韩 勇1，李小飞1   

  1. 1  解放军第四军医大学唐都医院胸腔外科，陕西省西安市 710038
    2  解放军第四军医大学组织工程研发中心，陕西省西安市 710032
  • 收稿日期:2012-04-16 修回日期:2012-07-24 出版日期:2013-01-08 发布日期:2013-01-08
  • 通讯作者: 李小飞，教授，主任医师，博士生导师，解放军第四军医大学唐都医院胸腔外科，陕西省西安市 710038 lxfchest@fmmu.edu.cn
  • 作者简介:吴凡★，男，1986 年生，陕西省西安市人，汉族，解放军第四军医大学在读硕士，医师，主要从事组织工程气管的研究。wf-315@163.com

Comparing three methods of preparing tissue engineering tracheal matrix

Wu Fan1, Zhang Zhi-pei1, Wang Jian1, Li Jian-zhong1, Jin Yan2, Jiang Tao1, Han Yong1, Li Xiao-fei1   

  1. 1 Department of Thoracic Surgery, Tangdu Hospital of the Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China
    2 Tissue engineering Research and Development Center of the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China
  • Received:2012-04-16 Revised:2012-07-24 Online:2013-01-08 Published:2013-01-08
  • Contact: Li Xiao-fei, Professor, Chief physician, Doctoral supervisor, Department of Thoracic Surgery, Tangdu Hospital of the Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China lxfchest@fmmu.edu.cn
  • About author:Wu Fan★, Studying for master’s degree, Physician, Department of Thoracic Surgery, Tangdu Hospital of the Fourth Military Medical University, Xi’an 710038, Shaanxi Province, China wf-315@163.com

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摘要：

背景：气管支架制备是组织工程学方法修复长段气管缺损的关键步骤。
目的：通过比较分析3种制备异体脱细胞气管支架的方法，为组织工程气管支架制备寻找更适宜的途径。
方法：手术获得兔新鲜气管，分为对照组、玻璃化液冷冻法组、酶洗法组、改良玻璃化液冷冻法组。处理后对各组标本行苏木精-伊红染色，电镜扫描观察，并测量气管最大拉伸力、破裂力和组织拉伸率等生物力学性能。
结果与结论：组织学观察显示对照组、玻璃化液冷冻法组可见部分完整黏膜上皮细胞，酶洗法组、改良玻璃化液冷冻法组未见黏膜上皮细胞。电镜观察示对照组、玻璃化液冷冻法组、改良玻璃化液冷冻法组有丰富的细胞外基质和胶原纤维，而酶洗法组无细胞外基质，只有胶原纤维。组间两两比较，气管支架的最大拉伸力、最大破裂力和组织拉伸率比较，差异均无显著性意义。说明应用改良玻璃化液冷冻法制备气管支架能够有效地去除抗原性、保留细胞外基质，并维持生物力学性能，是一种较为理想的组织工程气管支架制备方法。

关键词: 组织构建, 组织构建基础实验, 气管支架, 酶洗法, 玻璃化液冷冻法, 改良的玻璃化液冷冻法, 细胞外基质, 组织构建图片文章

Abstract:

BACKGROUND: It is a key step to prepare tracheal matrices in tissue engineering for repairing long segment tracheal defects.
OBJECTIVE: To find the better method for preparing tissue engineering tracheal matrices through comparing and analyzing three different ways of preparing allogeneic acellular tracheal matrices.
METHODS: Fresh rabbit tracheas were obtained by surgical operation and divided to four groups: untreated group, conventional vitrificational group, detergent-enzymatic group, modified vitrificational group. Hematoxylin-eosin staining and scanning electron microscopy were used to observe the morphology and ultrastructure of the treated tracheal matrices. The biomechanical properties including maximum tensile force, rupture force and tissue elongation of the treated tracheal matrices were measured.
RESULTS AND CONCLUSION: Histological observations showed that there were epithelial cells in the untreated and conventional vitrificational groups, but there were no epithelial cells in the detergent-enzymatic and modified vitrificational groups. There were abundant extracellular matrices and collagen fibers in the untreated group, conventional vitrificational group, and modified vitrificational group under electromicroscopy. Conversely, no extracellular matrix was found in the detergent-enzymatic group, in which there were only collagen fibers. Finally, no statistical differences were found in the maximum tensile force, rupture force and tissue elongation among all the groups. The method of modified vitrification, by which the antigen can be removed, and the extracellular matrix and biomechanical properties can be maintained effectively, is a more ideal way to prepare tissue engineering tracheal matrix.

Key words: tissue construction, basic experiments of tissue construction, tracheal scaffold, enzymatic washing, conventional vitrificational method, modified vitrificational method, extracellular matrix, tissue construction photographs-containing paper

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