中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (43): 8078-8082.doi: 10.3969/j.issn.2095-4344.2012.43.021

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心肌细胞与胶原材料复合再造组织工程心肌移植心肌梗死大鼠的心肌电生理变化

孙 娟1，陈晓洋2，张 玲1，黄 焰3   

  1. 1新疆医科大学第一附属医院，新疆维吾尔自治区乌鲁木齐市 830054
    2新疆维吾尔自治区人民医院，新疆维吾尔自治区乌鲁木齐市 830001
    3上海华东医院，上海市 200040
  • 收稿日期:2012-01-21 修回日期:2012-03-15 出版日期:2012-10-21 发布日期:2012-10-21
  • 通讯作者: 黄焰，博士，副教授，副主任医师，上海华东医院，上海市 200040 yhuang_look@ 126.com
  • 作者简介:孙娟☆，女，1979年生，陕西省西安市人，汉族，2009年新疆医科大学毕业，博士，主要从事心律失常的诊断及机制研究。 sunjuandee@ 126.com 并列第一作者：陈晓洋，男，1978年生，陕西省西安市人，新疆维吾尔自治区乌鲁木齐人，俄罗斯族，2002年石河子大学毕业，主治医师，主要从事心脏电生理研究。 ShouWANGZHE2046@sina.com

Electrophysiological changes following transplantation of tissue engineered myocardial tissue constructed by the compound of myocardial cells and collagen materials in rats with myocardial infarction

Sun Juan1, Chen Xiao-yang2, Zhang Ling1, Huang Yan3   

  1. 1First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China
    2People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, Xinjiang Uygur Autonomous Region, China
    3Huadong Hospital Affiliated to Fudan University, Shanghai 200040, China
  • Received:2012-01-21 Revised:2012-03-15 Online:2012-10-21 Published:2012-10-21
  • Contact: Huang Yan, Doctor, Associate professor, Associate chief physician, Huadong Hospital Affiliated to Fudan University, Shanghai 200040, China yhuang_look@126.com
  • About author:Sun Juan☆, Doctor, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, Xinjiang Uygur Autonomous Region, China sunjuandee@ 126.com Chen Xiao-yang, Attending physician, People’s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, Xinjiang Uygur Autonomous Region, China ShouWANGZHE2046@sina.com

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被引次数

1

摘要：

背景：组织工程化心肌组织移植可修复大鼠心肌梗死。
目的：应用微电极阵标测技术分析心肌细胞与胶原材料复合组织工程再造心肌移植心肌梗死大鼠的心肌电生理特性。
方法：将成年SD大鼠随机分组：假手术组、模型组、移植组。后2组制作心肌梗死动物模型，假手术组仅开胸不结扎冠状动脉，移植组造模后移植心肌细胞与胶原材料复合组织。应用微电极阵标测技术记录心室肌场电位振幅，激活-恢复时间及激动传导时间。
结果与结论：①心室肌场主波振幅：与模型组相比，移植组梗死区、对立区及周围区部位增高(P < 0.05)。②激活-恢复时间：模型组与移植组梗死区与梗死周边区均明显延长，且梗死周边区小于梗死区(P < 0.05)。与模型组相比，移植组梗死区、对立区及周围区部位激活-恢复时间缩短(P < 0.05)。③心室前壁激动传导时间：移植组高于假手术组，但低于模型组(P < 0.05)。表明移植的心肌细胞/胶原复合体能改善心肌梗死大鼠心室肌收缩功能及电传导时间，部分修复心脏功能。

关键词: 微电极阵标测技术, 心肌梗死, 胶原, 组织工程心肌, 场电位, 激活-恢复时间

Abstract:

BACKGROUND: Transplantation of tissue engineered myocardial tissue can repair myocardial infarction in rats.
OBJECTIVE: To study the electrophysiological characteristics after the transplantation of tissue engineered myocardial tissue.
METHODS: Thirty SD rats were evenly divided into three groups: control group, model group and transplantation group. A rat model of myocardial infarction was established in the latter two groups. The control group underwent thoracotomy with no ligation of the coronary artery. The transplantation group was transplanted with the compound of myocardial cells and collagen materials after modeled. Amplitude, activation-recovery interval and activation-conduction duration at local myocardium were recorded with microeletrode arrays technique.
RESULTS AND CONCLUSION: ①Compared with the model group, the stationary field amplitude of ventricular myocardium in infarction zone, opposite zone and border zone in the transplantation group were significantly increased (P < 0.05). ②Activation-recovery interval in the model group, as well as infarction zone and infarcted border zone in the transplantation group were prolonged variously (P < 0.05). While the activation-conduction duration of infarction zone, opposite zone and border zone in the transplantation group were shorten compared with the model group. ③In terms of the activation-conduction duration of left ventricular anterior wall, the transplantation group was higher than the control group, but lower than the model group (P < 0.05). These findings suggest that the compound of myocardial cells and collagen materials transplanted can improve the contractile function of the ventricular muscle and electrical conduction time, as well as repair the heart partly.

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