中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (43): 8055-8060.doi: 10.3969/j.issn.2095-4344.2012.43.017

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

魔芋葡甘聚糖/透明质酸共混膜的体外细胞生物相容性

顾 邵，潘险峰，徐永清，陈 佳，陈庆华   

  1. 解放军成都军区昆明总医院骨科，昆明医科大学临床教学医院，云南省昆明市 650032
  • 收稿日期:2012-01-11 修回日期:2012-03-11 出版日期:2012-10-21 发布日期:2012-10-21
  • 通讯作者: 潘险峰，博士，副主任医师，解放军成都军区昆明总医院，昆明医科大学临床教学医院，云南省昆明市 650032 panxianfeng@ Yahoo.com.cn
  • 作者简介:顾邵★，男，1985年生，云南省昆明市人，汉族，昆明医科大学在读硕士，主要从事脊柱外科研究。 18788144001@ 126.com

In vitro cellular biocompatibility of konjac glucomannan-hyaluronic acid blend membrane

Gu Shao, Pan Xian-feng, Xu Yong-qing, Chen Jia, Chen Qing-hua   

  1. Department of Orthopedics, Kunming General Hospital, Chengdu Military Area Command of Chinese PLA, Clinical Teaching Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China
  • Received:2012-01-11 Revised:2012-03-11 Online:2012-10-21 Published:2012-10-21
  • Contact: Pan Xian-feng, Doctor, Associate chief physician, Department of Orthopedics, Kunming General Hospital, Chengdu Military Area Command of Chinese PLA, Clinical Teaching Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China panxianfeng@ Yahoo.com.cn
  • About author:Gu shao★, Studying for master’s degree, Department of Orthopedics, Kunming General Hospital, Chengdu Military Area Command of Chinese PLA, Clinical Teaching Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China 18788144001@ 126.com

PDF

432

被引次数

3

摘要：

背景：高分子材料透明质酸与魔芋葡甘聚糖均可用于防治术后粘连。
目的：观察兔骨髓间充质干细胞与魔芋葡甘聚糖-透明质酸共混膜复合培养的生物相容性。
方法：取第3代兔骨髓间充质干细胞与魔芋葡甘聚糖-透明质酸共混膜体外复合培养，倒置显微镜和扫描电镜观察复合程度，MTT法检测细胞增殖。体外定向外诱导魔芋葡甘聚糖-透明质酸共混膜上的骨髓间充质干细胞向脂肪细胞分化，油红O染色检测其分化效果。
结果与结论：骨髓间充质干细胞与魔芋葡甘聚糖-透明质酸共混膜修复材料复合良好，倒置显微镜和扫面电镜观察均可见细胞在共混膜上良好黏附与增殖；体外定向诱导魔芋葡甘聚糖-透明质酸共混膜上的骨髓间充质干细胞可向脂肪细胞分化，具有成脂潜能。说明魔芋葡甘聚糖-透明质酸共混膜与骨髓间充质干细胞具有良好的生物相容性。

关键词: 魔芋葡甘聚糖, 透明质酸, 骨髓间充质干细胞, 脂肪细胞, 硬膜, 粘连, 生物相容性, 生物材料

Abstract:

BACKGROUND: Hyaluronic acid and konjac glucomannan can be used for the prevention of adhesion after surgery.
OBJECTIVE: To investigate the biocompatibility of konjac glucomannan-hyaluronic acid blend membrane co-cultured with bone marrow mesenchymal stem cells (BMSCs) of rabbits.
METHODS: The passage 3 BMSCs were cultured with konjac glucomannan-hyaluronic acid blend membrane in vitro. Their compound degree was observed by inverted microscope and scanning electron microscopy. Cell proliferation was detected by MTT assay. The BMSCs on the konjac glucomannan-hyaluronic acid blend membrane were orientiatedly induced into adipocytes in vitro. Differentiation effect was detected by oil red O staining
RESULTS AND CONCLUSION: BMSCs were well cultured with konjac glucomannan-hyaluronic acid blend membrane. Good adhesion and proliferation was found under inverted microscope and scanning electron microscope. The BMSCs on the konjac glucomannan-hyaluronic acid blend membrane could be orientiatedly induced into adipocytes, and had odipogenic potential. These findings suggest that there is a good biocompatibility between BMSCs and the konjac glucomannan-hyaluronic acid blend membrane.

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