中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (38): 7107-7110.doi: 10.3969/j.issn.2095-4344.2012.38.016

• 材料生物相容性 material biocompatibility • 上一篇    下一篇

变形链球菌gcp基因失活菌株生物膜形成能力及在唾液包被羟基磷灰石表面的黏附率

闫文娟   

  1. 南方医科大学南方医院口腔科，南方医科大学口腔医学院，广东省广州市 510515
  • 收稿日期:2012-01-13 修回日期:2012-03-04 出版日期:2012-09-16 发布日期:2012-09-16
  • 作者简介:闫文娟☆，女，1973年生，山东省青州市人，汉族，2009年解放军第四军医大学毕业，博士，主治医师，主要从事龋病的预防研究。 ywj918@ sohu.com

Biomembrane formation ability in Streptococcus mutans with gcp gene inactivation strain and the adhesion rate on the surface of hydroxylapatite coated with saliva

Yan Wen-juan   

  1. Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China
  • Received:2012-01-13 Revised:2012-03-04 Online:2012-09-16 Published:2012-09-16
  • About author:Yan Wen-juan☆, Doctor, Attending physician, Department of Stomatology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, Guangdong Province, China ywj918@sohu.com

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526

被引次数

2

摘要：

背景：前期实验发现外源性单磷酸鸟苷环二聚体能够抑制变形链球菌生物膜的形成及其体外黏附能力。变形链球菌内部存在的单磷酸鸟苷环二聚体是否具有同样作用。
目的：成功构建了变形链球菌内部单磷酸鸟苷环二聚体相关基因gcp的失活菌株，观察gcp基因失活后变形链球菌生物学特性的改变。
方法：将gcp失活菌和野生菌菌悬液在96孔酶标板中厌氧培养48 h，用结晶紫染色，乙醇/丙酮混合液显色，测量A575 nm值，以定量反映生物膜形成量；荧光标记gcp失活菌和野生菌，与唾液包被的羟基磷灰石粉末共同孵育后，测定羟基磷灰石沉淀的荧光值，比较黏附率的差异。
结果与结论：gcp失活菌生物膜形成量低于野生菌，gcp失活菌在唾液包被羟基磷灰石表面的黏附率低于野生菌(P < 0.05)。提示gcp基因的失活可抑制变形链球菌生物膜形成能力及在生物体表面的黏附。

关键词: 变形链球菌, gcp, 基因失活, 生物膜, 黏附

Abstract:

BACKGROUND: Our studies have confirmed that the exogenous bis-(3'-5')-cyclic dimeric guanosinemonophosphate (c-di-GMP) could inhibit the formation and adherion ability of Streptococcus mutans biomembrane. However, it is not clear whether c-di-GMP inside of streptococcus mutans has the same effect.
OBJECTIVE: To construct gcp gene inactivation strain of c-di-GMP within Streptococcus mutans and to observe the biological characters of Streptococcus mutans with gcp gene inactivation strain.
METHODS: Streptococcus mutans with gcp gene inactivation strain and wildlife mushroom supension were cultured on 96-well plates in anaerobic environment for 48 hours. The attached bacteria were stained with crystal violet, and ethanol/acetone dissolved the precipitated stain. The absorbance value at 575 nm was measured to represent the amount of biomembrane formation; Streptococcus mutans with gcp gene inactivation strain and wildlife mushroom were labeled by fluorescence; and then co-cultured with the hydroxylapatite coated with saliva. The fluorescence value of hydroxylapatite was detected and the adhesion rates between them were compared.
RESULTS AND CONCLUSION: The number of biomembranes in gcp gene inactivation strain was lower than that in wildlife mushroom; the adhesion rate on the surface of hydroxylapatite coated with saliva in gcp gene inactivation strain was lower than that in wildlife mushroom (P < 0.05). These findings suggest that the inactivation of gcp gene can inhibit the biomembrane formation of Streptococcus mutans and the adhesion on the surface of hydroxylapatite.

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