中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (37): 7009-7014.doi: 10.3969/j.issn.2095-4344.2012.37.033

• 组织构建学术探讨 tissue construction academic discussion • 上一篇    下一篇

软骨组织工程学方法修复关节软骨缺损

罗 文，范建楠，叶 川   

  1. 贵阳医学院附属医院骨科，贵州省贵阳市 550004
  • 收稿日期:2012-06-08 修回日期:2012-06-14 出版日期:2012-09-09 发布日期:2012-09-09
  • 通讯作者: 罗文★：男，1984年生，汉族，贵阳医学院在读硕士，主要从事骨与关节损伤研究。rovine1984@ yahoo.com.cn
  • 作者简介:罗文★：男，1984年生，汉族，贵阳医学院在读硕士，主要从事骨与关节损伤研究。rovine1984@ yahoo.com.cn 并列第一作者：范建楠，硕士生导师，副教授，副主任医师。 gyfysjb@163.com

Repairs of articular cartilage defects with cartilage tissue engineering methods

Luo Wen, Fan Jian-nan, Ye Chuan   

  1. Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China
  • Received:2012-06-08 Revised:2012-06-14 Online:2012-09-09 Published:2012-09-09
  • Contact: Luo Wen, Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China rovine1984@yahoo.com.cn
  • About author:Luo Wen★, Studying for master’s degree, Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China rovine1984@yahoo.com.cn Fan Jian-nan, Master’s supervisor, Associate professor, Associate chief physician, Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China gyfysjb@163.com Luo Wen and Fan Jian-nan contributed equally to this study.

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528

被引次数

11

摘要：

背景：关节软骨缺损在临床上十分常见，随着分子生物学和组织工程学等学科的不断发展，为关节软骨缺损的修复提供了大量新的方法和思路，利用软骨组织工程学方法修复关节软骨缺损成为目前骨科领域研究的热点。
目的：总结并讨论目前软骨组织工程学方法修复关节软骨缺损的研究现状，综述应用软骨组织工程学方法修复关节软骨缺损的研究进展。
方法：由第一作者应用计算机检索中国期刊全文数据库(CNKI：2000/2010)和Medline(1990/2010)数据库中的相关文章，检索词分别为“关节软骨缺损，软骨组织工程”和“articular cartilage defects (ACD)，cartilage tissue engineering”，语言分别设定为中文和英文。共检索得文章786篇，从中选取相关文章44篇，从软骨组织工程学方法修复关节软骨缺损过程中的种子细胞、支架材料和生物因子3个具体方面的研究进展进行归纳及总结。
结果与结论：支架、种子细胞和可调控细胞生长、增殖及分化的生物因子是软骨组织工程的3大要素。软骨组织工程方法主要包括利用体外培养、扩增后的种子细胞，将其种植于支架材料中，在相关调节因素的作用下形成组织工程化软骨，此方法已成为目前治疗关节软骨缺损的重要方法之一，并取得不错的疗效，但迄今为止尚未出现一种法被广泛认可的治疗方案。利用不同新型复合支架材料如修复治疗关节软骨缺损将成为今后研究的主要方向。

关键词: 关节软骨缺损, 软骨组织工程, 支架, 治疗, 研究进展, 文献综述

Abstract:

BACKGROUND: Articular cartilage defects are very common in clinic. With the development of molecular biology and tissue engineering, there are many new methods to repair articular cartilage defects. In recent years, the researches on repairs of articular cartilage defects with the cartilage tissue engineering method have obtained the satisfactory curative effect.
OBJECTIVE: To summarize and discuss the studies addressing repairs of articular cartilage defects with the cartilage tissue engineering methods.
METHODS: The first author searched relevant literature from CNKI (2000/2010) and Medline database (1990/2010). The key words were “articular cartilage defects, cartilage tissue engineering” in Chinese or English. Totally 786 articles were retrieved, and finally 47 articles were included. From aspects of seed cells, scaffold materials and biological factors, we generalized articles about the repairs of in the repair of articular cartilage defects with the cartilage tissue engineering methods.
RESULTS AND CONCLUSION: Scaffold, seed cells and biological factor are the three main elements of cartilage tissue engineering. The cartilage tissue engineering methods refer to implantation of in vitro cultured and proliferated seed cells onto the scaffolds to form tissue-engineered bone induced by relevant regulatory factors. The results show that the cartilage tissue engineering has become one of the important methods for the treatment of articular cartilage defects. Although the treatment of articular cartilage defects has obtained a good effect, there is not a widely recognized treatment program until now. Growth factors, such as bone morphogenetic protein and transforming growth factor β, can play a combined role in chondrocytes in autocrine and paracrine manners and can be intertwined to form a network, showing a temporal-spatial distribution. The use of new composite scaffolds to repair articular cartilage defects will become the main direction for future researches.

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