中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (37): 6973-6978.doi: 10.3969/j.issn.2095-4344.2012.37.027

• 组织构建基础实验 basic experiments in tissue construction • 上一篇    下一篇

大鼠角膜碱烧伤后热休克蛋白70的表达

张熙伯1，覃 纲2，徐 梅1，余 玲1   

  1. 泸州医学院附属医院，1眼科，2耳鼻喉科，四川省泸州市 646000
  • 收稿日期:2011-12-20 修回日期:2012-03-10 出版日期:2012-09-09 发布日期:2012-09-09
  • 通讯作者: 余玲，博士，副教授，泸州医学院附属医院眼科，四川省泸州市 646000
  • 作者简介:张熙伯，男，1966年生，四川省渠县人，汉族，副教授，主要从事角膜病的研究。

Expression of heat shock protein 70 in rat cornea during wound healing following alkali burn

Zhang Xi-bo1, Qin Gang2, Xu Mei1, Yu Ling1   

  1. 1Department of Ophthalmology, 2Department of Otolaryngology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China
  • Received:2011-12-20 Revised:2012-03-10 Online:2012-09-09 Published:2012-09-09
  • Contact: Yu Ling, Doctor, Associate professor, Department of Ophthalmology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China oculistlingyu@hotmail.com
  • About author:Zhang Xi-bo, Associate professor, Department of Ophthalmology, Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China Zhangxibo1966@163.com

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摘要：

背景：角膜碱烧伤后损伤修复受许多因素的影响，热休克蛋白可促进变性、损伤蛋白质的迅速恢复或清除。
目的：观察大鼠角膜碱烧伤后热休克蛋白70的表达及其与角膜损伤修复的关系。
方法：检查大鼠眼无炎症及其他病变后，奥布卡因滴眼液点眼2次，棉签吸除结膜囊液体，将统一规格直径5 mm的滤纸片浸泡于1 mol/L NaOH溶液中10 s，然后置于大鼠角膜中央30 s制作大鼠角膜碱烧伤模型。分别于碱烧伤后6 h，1，3，7，14，21 d取材。
结果与结论：RT-PCR、免疫组织化学染色、Western blot结果均显示热休克蛋白70 mRNA和蛋白在角膜碱烧伤后1 d即开始升高，7 d时达高峰，14 d后开始下降。苏木精-伊红染色及电镜观察显示角膜损伤在烧伤后6 h即较明显，烧伤后7 d逐渐恢复。提示大鼠角膜碱烧伤后热休克蛋白70的表达与碱烧伤后角膜损伤修复过程一致，参与了大鼠角膜碱烧伤后细胞的自我保护及修复过程。

关键词: 角膜, 碱烧伤, 热休克蛋白, 大鼠, 修复

Abstract:

BACKGROUND: Many factors can influence the wound healing of cornea following alkali burn, heat shock protein can promote the rapidly recovery and clear of the degenerated and injured protein.
OBJECTIVE: To investigate whether the expression of heat shock protein 70 after corneal alkali burn in rats is related to corneal wound healing process.
METHODS: After the rat eye was checked without inflammation and other lesions, oxybuprocaine eye drops were dropped into the rat eye for twice. Conjunctival sac liquid was sucked by swab, and then the unified specification filter paper of 5 mm diameter was soaked in 1 mol/L NaOH solution for 10 seconds, then the filter paper was placed in the center of the rat cornea for 30 seconds to make the rat corneal alkali burn model. The corneal specimens were obtained at 6 hours, 1, 3, 7, 14 and 21 days after alkali burn.
RESULTS AND CONCLUSION: Reverse transcription polymerase chain reaction, immunohistochemistry staining and Western blot observation showed that the expression level of heat shock protein 70 increased at 1 day after alkali burn and reached its peak at 7 days. Then, it decreased at 14 days. Hematoxylin-eosin staining and transmission electron microscope observation showed that the corneal injury was most obvious at 6 hours after burning and the wound was gradually recovered at 7 days after burning. Alkali burned cornea can induce heat shock protein 70 synthesis in a time-dependent manner, also in accordance with the corneal repair procedure. Induced heat shock protein 70 plays an important role in wound healing.

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