中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (37): 6921-6925.doi: 10.3969/j.issn.2095-4344.2012.37.017

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

不同浓度尿酸钠对大鼠滑膜细胞炎性因子的影响

孙贵才1，徐轶尔2，于雪峰1，吴洪亮1，凌小鹏1   

  1. 1南昌大学第四附属医院骨科，江西省南昌市 330003
    2黑龙江中医药大学研究生学院，黑龙江省哈尔滨市 150040
  • 收稿日期:2012-01-12 修回日期:2012-02-23 出版日期:2012-09-09 发布日期:2012-09-09
  • 作者简介:孙贵才☆，男，1970年生，黑龙江省庆安县人，汉族，2007年黑龙江中医药大学毕业，博士，副主任医师，主要从事关节外科方面的研究。 xuelei010207@126@.com

Influence of different concentrations of monosodium urate on inflammatory factors in synoviocytes in vitro

Sun Gui-cai1, Xu Yi-er2, Yu Xue-feng1, Wu Hong-liang1, Ling Xiao-peng1   

  1. 1Department of Orthopedics, Fourth Affiliated Hospital of Nanchang University, Nanchang 330003, Jiangxi Province, China
    2Graduate School of Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, Heilongjiang Province, China
  • Received:2012-01-12 Revised:2012-02-23 Online:2012-09-09 Published:2012-09-09
  • About author:Sun Gui-cai☆, Doctor, Associate chief physician, Department of Orthopedics, Fourth Affiliated Hospital of Nanchang University, Nanchang 330003, Jiangxi Province, China xulei010207@126.com

PDF

470

被引次数

4

摘要：

背景：利用尿酸钠刺激滑膜细胞制备体外急性痛风性关节炎滑膜模型，在评价治疗这类疾病药物作用机制方面有重要意义。
目的：通过不同浓度尿酸钠体外直接刺激滑膜细胞后滑膜细胞细胞因子的浓度变化，探讨急性痛风性关节炎滑膜模型制备条件。
方法：培养大鼠滑膜细胞，分别添加终浓度为0(空白组)，50，125，250，500，1 000 μmol/L的尿酸钠进行干预，于培养24，48 h后测定细胞活力及培养液中细胞因子白细胞介素1β、白细胞介素8、肿瘤坏死因子α的浓度。
结果与结论：各组在24，48 h内药物对细胞活力无影响。与空白组比较，尿酸钠干预组在24，48 h培养液中细胞因子白细胞介素1β、白细胞介素8、肿瘤坏死因子α浓度均提高，尿酸钠浓度为500 μmol/L，给药时间为24 h，培养液中各细胞因子浓度最高。说明利用一定浓度尿酸钠和刺激时间，便于筛选理想的急性痛风性关节炎体外模型。

关键词: 尿酸钠, 大鼠, 滑膜细胞, 细胞因子, 痛风性关节炎滑膜模型, 白细胞介素1β, 白细胞介素8, 肿瘤坏死因子α

Abstract:

BACKGROUND: Monosodium urate stimulates synoviocytes to prepare an in vitro model of acute gouty arthritis, which is important in evaluating of action mechanisms of this kind of drugs.
OBJECTIVE: To discuss the influential factors of establishing a model of acute gouty arthritis by directly stimulating synoviocytes in vitro with different concentrations of monosodium urate and observing concentration changes of cytokine.
METHODS: Synoviocytes from rats were treated with 0 (control group), 50, 125, 250, 500, 1 000 μmol/L monosodium urate. At 24 and 48 hours, cell viability and levels of interleukin-1 beta, interleukin-8, and tumor necrosis factor alpha in culture medium were detected.
RESULTS AND CONCLUSION: There were no effects on cell viability within 24 and 48 hours in control group and treatment groups. Compared with the control group, levels of interleukin-1 beta, interleukin-8, and tumor necrosis factor alpha in the medium of treatment groups were all increased, while the highest concentration was found when drug concentration was 500 μm/L and dosing interval was 24 hours. Monosodium urate at a certain concentration and dosing interval contributes to the screening of ideal models of acute gouty arthritis.

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