中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (33): 6137-6142.doi: 10.3969/j.issn.2095-4344.2012.33.012

• 皮肤粘膜组织构建 skin and mucosal tissue construction • 上一篇    下一篇

选择性环氧合酶2抑制剂尼美舒利抑制皮肤瘢痕形成的研究

潘 丽，涂腊根，罗嘉伦   

  1. 广州医学院护理学院，广东省广州市 510180
  • 收稿日期:2011-12-06 修回日期:2011-12-06 出版日期:2012-08-12 发布日期:2012-08-12
  • 通讯作者: 涂腊根，副教授，广州医学院护理学院，广东省广州市 510180 lagentu@163.com
  • 作者简介:潘丽★，女，1975年生，辽宁省葫芦岛市人，汉族，2006年暨南大学毕业，硕士，讲师，主要从事皮肤创伤愈合的研究。 panlide292@163.com 共同第一作者：罗嘉伦，主要从事皮肤创伤愈合的研究。

Effect of selective cyclooxygenase-2 inhibitor nimesulide on the formation of skin scar

Effect of selective cyclooxygenase-2 inhibitor nimesulide on the formation of skin scar   

  1. Guangzhou Medical College of Nursing, Guangzhou 510180, Guangdong Province, China
  • Received:2011-12-06 Revised:2011-12-06 Online:2012-08-12 Published:2012-08-12
  • Contact: Tu La-gen, Associate professor, Guangzhou Medical College of Nursing, Guangzhou 510180, Guangdong Province, China lagentu@163.com
  • About author:Pan Li★, Master, Lecturer, Guangzhou Medical College of Nursing, Guangzhou 510180, Guangdong Province, China panlide292@163.com Luo Jia-lun, Guangzhou Medical College of Nursing, Guangzhou 510180, Guangdong Province, China Pan Li and Luo Jia-lun contributed equally to the paper

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503

被引次数

5

摘要：

背景：尼美舒利是一种选择性环氧合酶2抑制剂，可通过抑制环氧合酶2的合成、抑制前列腺素的合成、白细胞的介质释放等环节影响炎症的发生过程，具有解热、镇痛和抗炎作用。
目的：探讨尼美舒利抑制小鼠皮肤瘢痕形成的作用及机制。
方法：昆明种小鼠背部脊柱两侧建立皮肤损伤模型，随机分为模型组、25，50，100 μmol/L尼美舒利组，切口分别涂抹0.5 mL玻璃酸钠凝胶、25，50，100 μmol/L尼美舒利玻璃酸钠分散制剂。
结果与结论：应用25，50 μmol/L尼美舒利可以明显减小瘢痕组织的面积(P < 0.01)，但100 μmol/L尼美舒利却造成损伤面积增大(P < 0.05)，与模型组相比，不同剂量的尼美舒利治疗的小鼠在创伤形成第3，7，14天环氧合酶2的表达明显降低，且其中50和100 μmol/L尼美舒利治疗的小鼠创口微血管的生成速度明显降低(P < 0.05)，提示尼美舒利可以通过适度下调皮肤修复过程中环氧合酶2的表达，控制微血管的形成，达到抑制病理性瘢痕形成的治疗目的，但剂量过大(100 μmol/L)则可抑制创伤的愈合。

关键词: 尼美舒利, 瘢痕, 微血管密度, 环氧合酶2, 皮肤, 组织构建, 组织工程

Abstract:

BACKGROUND: Nimesulide is a kind of selective cyclooxygenase-2 inhibitor that can influence the inflammation through inhibiting the synthesis of cyclooxygenase-2 and prostaglandin and the release of leukocytes media, which has antipyretic, analgesic and anti-inflammatory effects.
OBJECTIVE: To investigate the inhibition effect and mechanism of selective cyclooxygenase-2 inhibitor nimesulide on the formation of skin scar in mice.
METHODS: The models of skin wound were established and divided into four groups: model group, 25 μmol/L nimesulide group, 50 μmol/L nimesulide group and 100 μmol/L nimesulide group. The incisions were treated with 0.5 mL sodium hyaluronate, 25, 50 and 100 μmol/L nimesulide sodium hyaluronate dispersed preparation respectively.
RESULTS AND CONCLUSION: Nimesulide have significant impact on the skin wound healing, the scar area was significantly decreased in 25 μmol/L nimesulide group and 50 μmol/L nimesulide group (P < 0.01), but the scar area in the 100 μmol/L nimesulide group was increased (P < 0.05). The expressions of cyclooxygenase-2 in the nimesulide groups were significantly decreased at 3, 7 and 14 days after wound formation when compared with the model group, and the microvessel density in the 50 μmol/L nimesulide group and 100 μmol/L nimesulide group was significantly decreased (P < 0.05). Appropriate application of nimesulide has a positive effect on surgical wound in mice, and can reduce the pathological scar formation. But the overdose of nimesulide (100 μmol/L) can inhibit the healing of the wounds. The mechanism may be to decrease the expression of cyclooxygenase-2 and the formation of capillary vessels.

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