关键词: 尼美舒利, 瘢痕, 微血管密度, 环氧合酶2, 皮肤, 组织构建, 组织工程

Abstract:

BACKGROUND: Nimesulide is a kind of selective cyclooxygenase-2 inhibitor that can influence the inflammation through inhibiting the synthesis of cyclooxygenase-2 and prostaglandin and the release of leukocytes media, which has antipyretic, analgesic and anti-inflammatory effects.
OBJECTIVE: To investigate the inhibition effect and mechanism of selective cyclooxygenase-2 inhibitor nimesulide on the formation of skin scar in mice.
METHODS: The models of skin wound were established and divided into four groups: model group, 25 μmol/L nimesulide group, 50 μmol/L nimesulide group and 100 μmol/L nimesulide group. The incisions were treated with 0.5 mL sodium hyaluronate, 25, 50 and 100 μmol/L nimesulide sodium hyaluronate dispersed preparation respectively.
RESULTS AND CONCLUSION: Nimesulide have significant impact on the skin wound healing, the scar area was significantly decreased in 25 μmol/L nimesulide group and 50 μmol/L nimesulide group (P < 0.01), but the scar area in the 100 μmol/L nimesulide group was increased (P < 0.05). The expressions of cyclooxygenase-2 in the nimesulide groups were significantly decreased at 3, 7 and 14 days after wound formation when compared with the model group, and the microvessel density in the 50 μmol/L nimesulide group and 100 μmol/L nimesulide group was significantly decreased (P < 0.05). Appropriate application of nimesulide has a positive effect on surgical wound in mice, and can reduce the pathological scar formation. But the overdose of nimesulide (100 μmol/L) can inhibit the healing of the wounds. The mechanism may be to decrease the expression of cyclooxygenase-2 and the formation of capillary vessels.