中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (32): 5978-5982.doi: 10.3969/j.issn.2095-4344.2012.32.017

• 干细胞移植 stem cell transplantation • 上一篇    下一篇

毛囊干细胞对坐骨神经损伤修复的影响

柯锦城，程 波   

  1. 福建医科大学附属第一医院，福建省福州市 350005
  • 收稿日期:2012-01-11 修回日期:2012-03-30 出版日期:2012-08-05 发布日期:2012-08-05
  • 通讯作者: 程波，博士，教授，主任医师，福建医科大学附属第一医院，福建省福州市 350005
  • 作者简介:柯锦城★，男，1982年生，福建省泉州市安溪县人，汉族，福建医科大学在读硕士，主要从事毛囊干细胞方面的研究。 ranranxinzhu@163.com

Hair follicle stem cells in repair of sciatic nerve injury

Ke Jin-cheng, Cheng Bo   

  1. First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China
  • Received:2012-01-11 Revised:2012-03-30 Online:2012-08-05 Published:2012-08-05
  • Contact: 程波，博士，教授，主任医师，福建医科大学附属第一医院，福建省福州市 350005
  • About author:Ke Jin-cheng★, Studying for master’s degree, First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, Fujian Province, China ranranxinzhu@163.com

PDF

428

被引次数

8

摘要：

背景：毛囊干细胞具有多分化潜能，可分化成神经细胞，极有希望成为治疗周围神经损伤的种子细胞。
目的：观察毛囊干细胞对坐骨神经损伤修复的影响。
方法：体外分离培养SD大鼠乳鼠胡须处的毛囊干细胞，经鉴定备用。36只SD大鼠随机分为实验组和对照组，建立坐骨神经损伤模型后，实验组于坐骨神经损伤处的上方注入浓度约106 L-1的毛囊干细胞50 μL，对照组注射等量的磷酸盐缓冲液。
结果与结论：各组坐骨神经功能指数均随观察时间进行性增加，其中实验组大鼠神经功能恢复早于对照组；免疫组织化学检测移植后的毛囊干细胞大量存活并分化成神经细胞。结果提示毛囊干细胞能够有效的促进损伤的坐骨神经修复。

关键词: 毛囊干细胞, 坐骨神经损伤, 免疫组化, 坐骨神经功能指数, 神经细胞

Abstract:

BACKGROUND: Hair follicle stem cells are multipotent which can differentiate into neurons and repair injured peripheral nerves.
OBJECTIVE: To investigate the effects of hair follicle stem cells on repair of injured sciatic nerve.
METHODS: Hair follicle stem cells were isolated and cultured in vitro. Thirty-six healthy rats were selected to prepare sciatic nerve crush injury models with clamping method. Subsequently, rats were randomly divided into a transplantation group and a control group, with eighteen rats in each group. Rats in the transplantation group underwent transplantation of 50 μL of hair follicle stem cells at a concentration of 106/L into nerve injured sites. At the same time, equal amount of PBS was injected into the rats in the control group.
RESULTS AND CONCLUSION: Nestin, CK19, anti-integrin beta 1 positive cells could be found at injured nerve in the transplantation group 4 and 8 weeks after surgery. Sciatic nerve function indices in the transplantation group superiorly recovered to those in the control group 3-6 weeks after surgery. Hair follicle stem cells transplanted into injured nerve tissue promote the recovery of injured sciatic nerve.

中图分类号: