中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (27): 5092-5096.doi: 10.3969/j.issn.2095-4344.2012.27.029

• 干细胞基础实验 basic experiments of stem cells • 上一篇    下一篇

不同分娩方式不同性别新生儿脐血的免疫功能检测

刘克芹1，刘 晨2，尹卫东3，张 斌1   

  1. 1河北北方学院附属第一医院，河北省张家口市 075000；
    2武装警察部队北京总队医院，北京市 100027；
    3河北北方学院，河北省张家口市 075000
  • 收稿日期:2012-01-11 修回日期:2012-01-22 出版日期:2012-07-01 发布日期:2013-11-01
  • 通讯作者: 尹卫东，硕士，教授，河北北方学院，河北省张家口市 075000 yinweidong1531@sina.com
  • 作者简介:刘克芹，女，1968年生，河北省霸州市人，汉族，1992年张家口医学院毕业，副主任技师，主要从事医学检验诊断学研究。 yinweidong1531@sina.com
  • 基金资助:

    河北省卫生厅指令性计划项目(20090185)

Immune function detection of umbilical cord blood from newborns of different genders between cesarean section and vaginal birth

Liu Ke-qin1, Liu Chen2, Yin Wei-dong3, Zhang Bin1   

  1. 1The First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China;
    2The Armed Police Corps Hospital in Beijing, Beijing 100027, China;
    3Hebei North University, Zhangjiakou 075000, Hebei Province, China
  • Received:2012-01-11 Revised:2012-01-22 Online:2012-07-01 Published:2013-11-01
  • Contact: Yin Wei-dong, Master, Professor, Hebei North University, Zhangjiakou 075000, Hebei Province, China yinweidong1531@sina.com
  • About author:Liu Ke-qin, Associate chief technician, the First Affiliated Hospital of Hebei North University, Zhangjiakou 075000, Hebei Province, China yinweidong1531@sina.com

PDF

479

被引次数

26

摘要：

背景：影响新生儿免疫功能有多种因素。新生儿阴道分娩娩出时，子宫收缩会使胎儿处于相对缺血、缺氧状态，新生儿免疫状态受分娩方式的影响；剖宫产新生儿免疫力低，易继发感染。
目的：对比分析剖宫产与阴道分娩对不同性别新生儿脐血免疫功能的影响。
方法：选择足月单胎分娩产妇100例，其中阴道分娩组50例，新生儿男性30例，女性20例；剖宫产组50例，新生儿男性30例，女性20例。采用自动生化分析仪透射免疫浊度法检测两组新生儿脐血免疫球蛋白(IgG、IgA、IgM)、补体(C3、C4)及C-反应蛋白水平，流式细胞仪酶标法测定T淋巴细胞抗原(CD4、CD8)水平。
结果与结论：剖宫产组女性婴儿脐血淋巴细胞CD4抗原、CD4与CD8混合抗原水平显著高于阴道分娩组(P < 0.01)，CD8抗原水平却显著低于阴道分娩组(P < 0.01)；剖宫产组女性婴儿脐血IgA、IgM水平显著高于男性组(P < 0.01)，IgG水平却低于男性组(P < 0.05)。剖宫产组女性婴儿脐血IgG、
IgM、C-反应蛋白水平显著低于阴道分娩组(P < 0.01)，男性婴儿脐血IgM水平显著低于阴道分娩组(P < 0.01)，C-反应蛋白水平显著高于阴道分娩组(P < 0.01)。提示不同分娩方式对不同性别新生儿免疫功能有影响，剖宫产可降低新生儿的免疫功能。

关键词: 新生儿, 脐血, 免疫球蛋白, C-反应蛋白, 分娩方式

Abstract:

BACKGROUND: There have been many factors that influence neonatal immune function. In vaginal birth, uterine contraction makes the newborn child in a relative ischemic hypoxic status, and the type of delivery can influence the immune status of the newborn children. The newborn children delivered by cesarean section have low immunity and predispose to secondary infection.
OBJECTIVE: To explore the influence of cesarean section versus vaginal birth on immune function of umbilical cord blood of newborn children of different genders.
METHODS: 100 cases of women who had full-term single birth were selected, including 50 cases subjected to vaginal delivery (30 male babies and 20 female babies) and 50 cases subjected to cesarean section (30 male babies and 20 female babies). Umbilical cord blood immunoglobulin (IgG, IgA, IgM), the complement (C3, C4), C-reactive protein of newborn children were detected by transmission immune turbidity method using automatic biochemical analyzer. T lymphocyte antigen (CD4, CD8) was determined by flow cytometry.
RESULTS AND CONCLUSION: In the cesarean section group, the level of umbilical cord blood lymphocyte CD4 antigen and the mixed antigen CD4 and CD8 in female babies was significantly higher (P < 0.01), but CD8 antigen level was significantly lower (P < 0.01), than in the vaginal birth group. In the cesarean section group, umbilical cord IgA and IgM level in female babies was significantly higher (P < 0.01), but IgG level was significantly lower (P < 0.05) than in male babies. Umbilical cord IgG, IgM, C-reactive protein levels of female babies in the cesarean section group were significantly lower than in the vaginal birth group (P < 0.01). In the cesarean section group, umbilical cord IgM level of male babies was significantly lower (P < 0.01), but C-reactive protein level of male babies was significantly higher (P < 0.01), than in the vaginal birth group (P < 0.01). These findings suggest that the type of delivery produces different influences on immune function of newborn children of different genders, and cesarean section can decrease the immune function of newborn children.

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