中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (29): 4749-4756.doi: 10.3969/j.issn.2095-4344.1819

• 干细胞综述 stem cell review • 上一篇    

骨髓间充质干细胞复合支架材料治疗骨缺损：研究现状及前景展望

池玉磊1，卜宪敏2，查玉梅3，王海滨3，孟纯阳3，吴  彬1，3   

  1. 1济宁医学院临床医学院，山东省济宁市  272000；2济宁市第一人民医院，山东省济宁市  272000；3济宁医学院附属医院骨科，山东省济宁市  272000
  • 修回日期:2019-05-17 出版日期:2019-10-18 发布日期:2019-10-18
  • 通讯作者: 吴彬，博士，副主任医师，济宁医学院，山东省济宁市 272000；济宁医学院附属医院骨科，山东省济宁市 272000
  • 作者简介:池玉磊，男，1990年生，山东省人，汉族，济宁医学院在读硕士，主要从事骨缺损修复方面的研究。 共同第一作者：卜宪敏，1980年生，山东省微山县人，汉族，2006年福建医科大学毕业，硕士，副主任医师，主要从事外科病理学研究。
  • 基金资助:

    国家自然科学基金(81572205)，项目负责人：孟纯阳；山东省自然科学基金(ZR2014CQ042)，项目负责人：吴彬；济宁医学院教师扶持基金(JYFC2018FKJ048)，项目负责人：吴彬；济宁医学院贺林院士基金(JYHL2018FMS13)，项目负责人：吴彬

Bone marrow mesenchymal stem cells combined with scaffolds in treatment of bone defects: present and future

Chi Yulei1, Bu Xianmin2, Zha Yumei3, Wang Haibin3, Meng Chunyang3, Wu Bin1, 3   

  1. 1Clinical Institute, Jining Medical University, Jining 272000, Shandong Province, China; 2Jining No. 1 People’s Hospital, Jining 272000, Shandong Province, China; 3Department of Orthopedics, Affiliated Hospital of Jining Medical University, Jining 272000, Shandong Province, China
  • Revised:2019-05-17 Online:2019-10-18 Published:2019-10-18
  • Contact: Wu Bin, MD, Associate chief physician, Clinical Institute, Jining Medical University, Jining 272000, Shandong Province, China; Department of Orthopedics, Affiliated Hospital of Jining Medical University, Jining 272000, Shandong Province, China
  • About author:Chi Yulei, Master candidate, Clinical Institute, Jining Medical University, Jining 272000, Shandong Province, China; Bu Xianmin, Master, Associate chief physician, Jining No. 1 People’s Hospital, Jining 272000, Shandong Province, China. Chi Yulei and Bu Xianmin contributed equally to this work.
  • Supported by:

    the National Natural Science Foundation of China, No. 81572205 (to MCY); Natural Science Foundation of Shandong Province, No. ZR2014CQ042 (to WB); Fund for Teachers Support of Jining Medical University, No. JYFC2018FKJ048 (to WB); Research Fund for Lin He’s Academician Workstation of New Medicine and Clinical Translation in Jining Medical University, No. JYHL2018FMS13 (to WB)

PDF

167

摘要：

文章快速阅读：

文题释义：
骨髓间充质干细胞的优势：是最早在骨髓中发现的异质性、原始细胞，具有易获取、易培养、低免疫原性和易于外源基因转染并长期表达等特点，来源较为方便，能够定向分化为成骨细胞、脂肪细胞和成软骨细胞等，目前被广泛应用于骨缺损的重建和再生，是治疗骨缺损的最佳种子细胞。
生物三维打印：是以活细胞、生物活性因子以及生物材料为基本成形单元，设计制造具有生物活性的人工器官、植入物或细胞三维结构，是制造科学与生物医学交叉融合的新兴学科，也是三维打印技术中最具活力和发展前景的方向。

 

摘要
背景：骨缺损是临床常见且较难解决的问题，骨髓间充质干细胞复合支架材料治疗骨缺损是目前研究的热点。
目的：总结骨髓间充质干细胞复合支架材料治疗骨缺损的最新研究进展，为临床应用提供理论依据。
方法：文章第一作者通过检索中国知网、万方、维普、PubMed、Embase和Web of Science数据库2009年1月至2018年12月相关文献，检索词为“骨髓间充质干细胞，分离培养，支架材料，骨缺损，骨组织工程，成骨分化，新生血管，骨膜，细胞外基质”“bone marrow mesenchymal stem cells，isolation，scaffolds，bone defects，bone tissue engineering，osteogenesis，angiogenesis，periosteum，extracellular matrix”。最终选取符合纳入标准的文献共计95篇。
结果与结论：大量研究表明，骨髓间充质干细胞是治疗骨缺损的理想种子细胞，其与支架材料复合能够促进骨缺损修复。目前，骨髓间充质干细胞复合支架材料治疗骨缺损正处于新的发展时代，是一种很有前景的骨缺损治疗方法，其中在骨髓间充质干细胞的分离培养、定向分化及支架材料研究方面取得了很大的进展，并进一步证明了新生血管、骨膜和细胞外基质在整个骨组织工程中的重要作用，这一研究对未来临床治疗骨缺损具有重大意义。


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0002-4187-1465(池玉磊)

关键词: 骨髓间充质干细胞, 支架材料, 骨缺损, 骨组织工程, 成骨分化, 新生血管, 骨膜, 细胞外基质, 国家自然科学基金

Abstract:

BACKGROUND: Bone defect is a common problem encountered in clinical practice. To this end, bone marrow mesenchymal stem cells combined with scaffolds is a hot topic in the treatment of bone defects.
OBJECTIVE: To summarize the latest research progress in bone marrow mesenchymal stem cells combined with scaffolds in the treatment of bone defects, which provides a theoretical basis for clinical application.
METHODS: The first author searched CNKI, WanFang, VIP, PubMed, Embase and Web of Science databases from January 2009 to December 2018. Search terms were “bone marrow mesenchymal stem cells, isolation, scaffolds, bone defects, bone tissue engineering, osteogenesis, angiogenesis, periosteum, extracellular matrix” in Chinese and English. Totals of 95 articles that met the inclusion criteria were selected.
RESULTS AND CONCLUSION: Numerous studies have shown that bone marrow mesenchymal stem cells are ideal seed cells for the treatment of bone defects, and cell-scaffold composites can promote the repair and healing of bone defects. To date, bone marrow mesenchymal stem cells combined with scaffolds as a promising treatment for bone defects have been in the new development era. Great progress has been achieved in terms of isolation, culture and directed differentiation of bone marrow mesenchymal stem cells as well as scaffold materials. These findings further indicate the important roles of new vessels, periosteum and extracellular matrix in bone tissue engineering, which are of great significance for the future clinical treatment of bone defects.

Key words: bone marrow mesenchymal stem cells, scaffold material, bone defect, bone tissue engineering, osteogenic differentiation, neovascularization, periosteum, extracellular matrix, National Natural Science Foundation of China

中图分类号: