中国组织工程研究

中国组织工程研究 ›› 2019, Vol. 23 ›› Issue (29): 4743-4748.doi: 10.3969/j.issn.2095-4344.1797

• 干细胞综述 stem cell review • 上一篇    下一篇

间充质干细胞条件培养液修复多种疾病损伤的潜能

邱纪玲1，2，王晓彤1，2，周灏雯1，2，杨丕山1，2，宋爱梅1，2   

  1. 1山东省口腔组织再生重点实验室，山东省济南市  250100；2山东大学口腔医学院，山东省济南市  250012
  • 修回日期:2019-04-15 出版日期:2019-10-18 发布日期:2019-10-18
  • 通讯作者: 宋爱梅，硕士生导师，山东省口腔组织再生重点实验室，山东省济南市 250100；山东大学口腔医学院，山东省济南市 250012
  • 作者简介:邱纪玲，女，1992年生，山东省人，汉族，山东大学口腔医学院在读硕士，主要从事牙周再生方面的研究。
  • 基金资助:

    国家自然科学基金(81771076)，项目负责人：杨丕山

Conditioned medium from cultured mesenchymal stem cells: a potential for repairing multiple diseases and injuries 

Qiu Jiling1, 2, Wang Xiaotong1, 2, Zhou Haowen1, 2, Yang Pishan1, 2, Song Aimei1, 2   

  1. 1Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250100, Shandong Province, China; 2School of Stomatology, Shandong University, Jinan 250012, Shandong Province, China
  • Revised:2019-04-15 Online:2019-10-18 Published:2019-10-18
  • Contact: Song Aimei, Master’s supervisor, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250100, Shandong Province, China; School of Stomatology, Shandong University, Jinan 250012, Shandong Province, China
  • About author:Qiu Jiling, Master candidate, Shandong Provincial Key Laboratory of Oral Tissue Regeneration, Jinan 250100, Shandong Province, China; School of Stomatology, Shandong University, Jinan 250012, Shandong Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81771076 (to YPS)

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摘要：

文章快速阅读：

文题释义：
间充质干细胞条件培养液：在细胞培养过程中，间充质干细胞可能会分泌活性物质到培养液中，其中细胞因子是主要的活性物质之一，这种培养过某种细胞或组织以后，含有细胞分泌物的培养液就叫做条件培养液。
牙槽骨垂直型吸收：也称角形吸收，指牙槽骨发生垂直方向或斜行的吸收，与牙根面之间形成一定角度的骨缺损，牙槽骨高度降低不多，而牙根周围骨吸收较多。大多形成骨下袋，最常见于邻面。根据骨质破坏后剩余的骨壁数目，骨下袋可分为一壁骨袋、二壁骨袋、三壁骨袋、四壁骨袋、混合骨袋。

 

摘要
背景：间充质干细胞为再生医学的发展和多种疾病的治疗提供了新思路、新方法，但由于干细胞在受植床的移植率和成活率均较低，其作用机制更多倾向于旁分泌效应。条件培养液含有干细胞分泌的多种活性物质，具有易于获取、使用方便、安全性高等优点，将有可能作为一种新的技术用于人类多种疾病的治疗。
目的：对间充质干细胞条件培养液的成分、功能、获取、应用等方面的进展做一综述，以期更好地应用于疾病治疗。
方法：在PubMed、CNKI、万方数据库中，英文检索词为“Mesenchymal Stem Cells AND Conditioned medium”，中文检索词为“间充质干细胞AND条件培养液，间充质干细胞AND条件培养基”，进行文献检索并筛选，最终纳入46篇进行综述。
结果与结论：间充质干细胞条件培养液中含有间充质干细胞在生长过程中分泌的生长因子、细胞因子、炎性因子、细胞外基质蛋白和组织重塑酶等多种活性物质, 具有趋化性能、免疫调节、促血管生成、细胞生长支持、保持干细胞活性或促进其他细胞转化为干细胞等功能。收集间充质干细胞生长过程中的培养液，经过过滤或浓缩等处理，可以获得不含细胞成分但含有更高浓度生长因子和细胞因子的活性液体，使用方便安全，现已将其用于基础研究、多种动物疾病模型和某些人体临床研究，并获得了较好的效果，随着对干细胞条件培养液的研究深入和不断优化，干细胞条件培养液的优势将会得到发挥，从而更好地造福于人类。


中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程
ORCID: 0000-0002-8484-1266(邱纪玲)

关键词: 间充质干细胞, 条件培养液, 旁分泌, 细胞因子, 生长因子, 再生医学, 国家自然科学基金

Abstract:

BACKGROUND: Mesenchymal stem cells have provided new ideas and new methods for the development of regenerative medicine and the treatment of a variety of diseases. However, due to the low transplantation rate and survival rate of stem cells in the implanted bed, the mechanism of action of stem cells is inclined to involve the paracrine effect of soluble molecules released by stem cells during wound healing. The conditioned medium contains a variety of active substances secreted by stem cells. It is characterized by being easy to obtain, easy to use and safe, and may be used as a new technology for the treatment of a variety of human diseases.
OBJECTIVE: To review the?composition, function, acquisition and application of conditioned medium from mesenchymal stem cells, attempting to better apply it in the treatment of diseases.
METHODS: The keywords were “mesenchymal stem cells AND conditioned medium” in English and Chinese, respectively. Relevant articles published were retrieved by the first author in the PubMed, CNKI, and WanFang. According to the inclusion and exclusion criteria, finally 46 articles were retained.
RESULTS AND CONCLUSION: Conditioned medium obtained from mesenchymal stem cells contains growth factors, cytokines, inflammatory factors, extracellular matrix proteins, and tissue remodeling enzymes that are secreted by mesenchymal stem cells during the growth. The conditioned medium has the functions of immunomodulation, promotes angiogenesis, maintains stem cell activity, supports the growth of cells, has chemoattraction properties, and promotes cell differentiation into stem cells. The culture medium from cultured mesenchymal stem cells can be filtrated or concentrated to be an active liquid containing no cell components but higher concentrations of growth factors and cytokines, which is convenient and safe to use. This medium has been used for the basic research, a variety of animal disease models and some human clinical studies, and has achieved good results. With the in-depth research the stem cell conditioned medium will be continuously developed and exert their advantages to better benefit humans.

Key words: mesenchymal stem cells, conditioned medium, paracrine, cytokines, growth factors, regenerative medicine, National Natural Science Foundation of China

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