中国组织工程研究

中国组织工程研究 ›› 2018, Vol. 22 ›› Issue (8): 1217-1222.doi: 10.3969/j.issn.2095-4344.0140

• 组织构建实验造模 experimental modeling in tissue construction • 上一篇    下一篇

莫诺苷通过抑制炎症反应改善脑出血模型大鼠的神经功能

袁志俊，何晓英，袁  平，郑晓梅，李小刚   

  1. 西南医科大学附属医院神经内科，四川省泸州市  646000
  • 收稿日期:2017-12-16 出版日期:2018-03-18 发布日期:2018-03-18
  • 通讯作者: 李小刚，主任医师，硕士研究生导师，西南医科大学附属医院神经内科，四川省泸州市 646000
  • 作者简介:袁志俊，女，1988年生，四川省泸州市人，2011年泸州医学院毕业，硕士，医师，主要从事神经病学方面的研究。
  • 基金资助:

    四川省卫生厅科研项目(110368)

Morroniside improves the neurological function in intracerebral hemorrhage rats by inhibiting inflammatory response

Yuan Zhi-jun, He Xiao-ying, Yuan Ping, Zheng Xiao-mei, Li Xiao-gang   

  1. Department of Neurology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Received:2017-12-16 Online:2018-03-18 Published:2018-03-18
  • Contact: Li Xiao-gang, Chief physician, Master’s supervisor, Department of Neurology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Yuan Zhi-jun, Master, Physician, Department of Neurology, the Affiliated Hospital of Southwest Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the Research Project of Sichuan Provincial Health Department, No. 110368

PDF

177

摘要：

文章快速阅读：

文题释义：
莫诺苷：是从中药山茱萸中分离提取的一种成分，据国内外研究表明它在大鼠缺血性脑损伤模型中，具有抗炎、抗氧化、抗凋亡、促进血管和神经再生、抗血小板聚集和神经保护作用。因脑出血的病理生理过程与缺血性脑损伤有许多相似之处，因此推测莫诺苷是否也可以抑制脑出血的炎症反应。
炎症反应：是临床常见的一个病理过程，可以生于机体各部位的组织和各器官。致炎因子作用于机体后，一方面引发组织细胞的损坏，使局部组织细胞显现变性、坏死；另一方面，诱导机体抗病机能增加，以益于清除致炎因子，使受损组织得到修复，从而使机体的内环境以及内环境和外环境之间达到新的均衡。
摘要
背景：研究表明莫诺苷在大鼠缺血性脑损伤模型中具有抗炎、抗氧化、抗凋亡、促进血管和神经再生、抗血小板聚集和神经保护作用，但莫诺苷是否可以抑制脑出血的炎症反应尚不清楚。
目的：观察不同剂量的莫诺苷对大鼠脑出血后炎症因子白细胞介素1、肿瘤坏死因子α和炎症相关蛋白NF-κB和SUMO2/3的影响及神经功能变化。
方法：将健康雄性SD大鼠随机分为假手术组、脑出血和莫诺苷低、中、高剂量组，后4组大鼠采用自体尾动脉血注入法建立脑出血模型，术后莫诺苷低、中、高剂量组分别给予莫诺苷30，90，270 mg/kg每天3次灌胃给药，连续7 d，假手术组和模型组给予等量生理盐水。给药7 d后应用NSS评分观察各组大鼠神经功能缺损症状；评分后取大鼠血肿周围脑组织，苏木精-伊红染色观察血肿周围神经细胞形态结构的变化；ELISA法检测大鼠脑组织炎症因子白细胞介素1、肿瘤坏死因子α水平；免疫组化法及Western blotting法检测通道蛋白NF-κB和SUMO2/3蛋白的表达。
结果与结论：①与脑出血组比较，莫诺苷低、中、高剂量组神经功能缺损改善，其中高剂量组改善最明显(P < 0.05)；②与假手术比较，脑出血组和莫诺苷低、中、高剂量组神经功能受损，炎症因子白细胞介素1、肿瘤坏死因子α和炎症相关蛋白NF-κB、SUMO2/3表达增多(P < 0.05)；③与脑出血组比较，莫诺苷低、中、高剂量组炎症因子白细胞介素1、肿瘤坏死因子α表达减少，炎症相关蛋白NF-κB降低，SUMO2/3表达增多(P < 0.05)，以高剂量组作用最明显(P < 0.05)。提示：高剂量莫诺苷可通过炎症调节蛋白的变化，下调炎症因子的表达，抑制炎症反应改善脑出血大鼠的神经功能。

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程
ORCID: 0000-0001-9255-7508(袁志俊)

关键词: 莫诺苷, SUMO2/3蛋白, 组织构建, 组织工程, 脑出血, NF-κB蛋白, 白细胞介素1, 肿瘤坏死因子α, 炎症反应

Abstract:

BACKGROUND: Morroniside has been shown to play roles of anti-inflammation, antioxidant, anti-apoptosis, promoting vascular and neural regeneration, anti-platelet aggregation and neuroprotection in the rat model of ischemic brain injury, but whether it can inhibit the inflammatory reaction of cerebral hemorrhage is unclear.
OBJECTIVE: To observe the changes of inflammatory factors (interleukin-1, tumor necrosis factor-α) and inflammatory-related proteins (nuclear factor-κB and SUMO2/3) as well as neurologic function in a rat model of cerebral hemorrhage treated with morroniside at different doses.
METHODS: Healthy male Sprague-Dawley rats were randomly divided into sham operation, cerebral hemorrhage and low-, medium- and high-dose morroniside groups. The model of cerebral hemorrhage was established in the latter four groups by injecting autologous blood from the tail artery, followed by intragastric injection of 30, 90, 270 mg/kg morroniside in the three morronicide groups, respectively, three times daily for consecutive 7 days; the rats in the sham operation and model groups were given same volume of normal saline. Then, the neurological function was evaluated by Neurological Severity Scores; the brain tissue around the hematoma were removed to observe the morphological changes of neurocytes around the hematoma by hematoxylin-eosin staining; the expression levels of interleukin-1 and tumor necrosis factor α in the brain tissue were detected by ELISA; the expression levels of nuclear factor-κB and SUMO2/3 were detected by immunohistochemistry and western blot assay.
RESULTS AND CONCLUSION: Compared with the icerebral hemorrhage group, the low-, medium- and high-dose morroniside groups showed a significnat neurological improvement, especially the high-dose group (P < 0.05). Compared with the sham operation group, the cerebral hemorrhage and morroniside groups exhibited a significant increase in the nerve function damage and expression levels of interleukin-1, tumor necrosis factor α, nuclear factor-κB and SUMO2/3 (P < 0.05). Compared with the cerebral hemorrhage group, in the low-, medium- and high-dose morroniside groups, the expression levels of interleukin-1 and tumor necrosis factor α were significantly reduced, and expression levels of nuclear factor-κB and SUMO2/3 were significantly increased (P < 0.05). In summary, high-dose morroniside can improve the neurological function in rats with cerebral hemorrhage by down-regulating the levels of inflammatory cytokines.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Cerebral Hemorrhage, Cornaceae, Small Ubiquitin-Related Modifier Proteins, NF-kappa B, Tumor Necrosis Factor-alpha

中图分类号: