中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4367-4370.doi: 10.3969/j.issn.1673-8225.2012.23.035

• 干细胞学术探讨 stem cell academic discussion • 上一篇    

人脐血单个核细胞移植前后自闭症患儿脑脊液中细胞生长因子的表达

李 强1，吕涌涛1，郇 英1，刘 敏1，葛汝村1，陈兴旺1，齐宏顺1，高宝峰1，胡 祥2，安 蕾2   

  1. 1山东省交通医院干细胞与再生医学研究室，山东省济南市 250031；
    2深圳市北科生物科技有限公司，广东省深圳市 518057
  • 收稿日期:2012-01-03 修回日期:2012-03-25 出版日期:2012-06-03 发布日期:2013-11-06
  • 作者简介:李强★，男，1972年生，山东省济南市人，汉族，2004年辽宁医学院毕业，硕士，副主任医师，主要从事脑血管病及神经再生修复的基础及临床研究。 Liqiang9651@yahoo.com.cn

Changes in cellular growth factor levels in cerebrospinal fluid of autism patients before and after human umbilical cord blood mononuclear cell transplantation

Li Qiang1, Lü Yong-tao1, Huan Ying1, Liu Min1, Ge Ru-cun1, Chen Xing-wang1, Qi Hong-Shun1, Gao Bao-Feng1, Hu Xiang2, An Lei2   

  1. 1Department of Stem Cells and Regeneration Medicine, Shandong Hospital of Communication, Jinan 250031, Shandong Province, China;
    2Shenzhen Beike Biotechnology Co.,Ltd., Shenzhen 518057, Guangdong Province, China
  • Received:2012-01-03 Revised:2012-03-25 Online:2012-06-03 Published:2013-11-06
  • About author:Li Qiang★, Master, Associate chief physician, Department of Stem Cells and Regeneration Medicine, Shandong Hospital of Communication, Jinan 250031, Shandong Province, China Liqiang9651@yahoo.com.cn

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摘要：

背景：研究发现脐血单个核细胞治疗自闭症具有一定的效果。
目的：观察人脐血单个核细胞移植治疗自闭症患儿脑脊液中神经生长因子、血管内皮细胞生长因子、碱性成纤维细胞生长因子水平的变化。
方法：经腰穿蛛网膜下腔途径，以2×106/kg异体人脐血单个核细胞移植治疗30例儿童自闭症患者，1次/周，共治疗3次。
结果与结论：第2次移植1周后患儿脑脊液中神经生长因子较移植前、第1次移植1周后水平增高(P < 0.05)，血管内皮细胞生长因子及碱性成纤维细胞生长因子水平均较移植前、第1次移植1周后水平增高，但差异无显著性意义 (P > 0.05)。说明人脐血单个核细胞移植治疗自闭症患儿脑脊液中神经生长因子水平明显增高，血管内皮细胞生长因子及碱性成纤维细胞生长因子变化不明显。

关键词: 脐血, 单个核细胞, 儿童, 自闭症, 脑脊液, 生长因子, 干细胞

Abstract:

BACKGROUND: Studies have demonstrated that umbilical cord blood mononuclear cells exhibit some effects in treatment of autism.
OBJECTIVE: To detect levels of nerve growth factor (NGF), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) in cerebrospinal fluid of autism patients treated with umbilical cord blood mononuclear cells.
METHODS: A total of 30 autism children received treatment by injection of human umbilical cord blood mononuclear cells via lumbar puncture subarachnoid cavity, once a week, for three times totally.
RESULTS AND CONCLUSION: The levels of nerve growth factors were significantly greater at 1 week after the second treatment than pretreatment and 1 week after the first treatment (P < 0.05). The levels of vascular endothelial growth factors and basic fibroblast growth factors were slightly, but not significantly, higher at 1 week after the second treatment than pretreatment and at 1 week after the first treatment (P > 0.05). Results suggest that the levels of nerve growth factors in cerebrospinal fluid were significantly increased in autism children who received treatment of umbilical cord blood mononuclear cells, however, the levels of vascular endothelial growth factors and basic fibroblast growth factors were not influenced significantly.

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