中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (23): 4204-4208.doi: 10.3969/j.issn.1673-8225.2012.23.005

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

人骨髓间充质干细胞及条件培养液对异体T淋巴细胞分泌功能的影响

白 海，吴 冰，王存邦，马晓慧，司志刚   

  1. 解放军兰州军区兰州总医院血液科，甘肃省兰州市 730050
  • 收稿日期:2011-12-09 修回日期:2012-02-17 出版日期:2012-06-03 发布日期:2013-11-06
  • 作者简介:白海☆，男，1962年生，山西省临县人，汉族，2004年解放军第四军医大学毕业，博士，主任医师，主要从临床和实验血液学研究。 baihai98@tom.com
  • 基金资助:

    军队”十一五”杰出人才基金资助项目(06J005)，课题名称：HLA半相合造血干细胞和间充质干细胞共移植治疗极重度骨髓型放射病研究

Effects of human bone marrow mesenchymal stem cells and their conditioned medium on the secretary function of allogenous peripheral blood T lymphocytes

Bai Hai, Wu Bing, Wang Cun-bang, Ma Xiao-hui, Si Zhi-gang   

  1. Department of Hematology, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu Province, China
  • Received:2011-12-09 Revised:2012-02-17 Online:2012-06-03 Published:2013-11-06
  • About author:Bai Hai☆, M.D., Chief physician, Department of Hematology, Lanzhou General Hospital of Lanzhou Military Area Command of Chinese PLA, Lanzhou 730050, Gansu Province, China baihai98@tom.com

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386

被引次数

7

摘要：

背景：骨髓间充质干细胞具有支持造血、多向分化及免疫调节作用，后者在异基因造血干细胞移植尤其是HLA半相合造血干细胞移植后移植物抗宿主病中具有潜在的应用价值，但其免疫调节作用的机制不清。
目的：体外观察人骨髓间充质干细胞及其条件培养液对异体外周血T淋巴细胞及其分泌功能的影响。
方法：从人骨髓中分离培养骨髓间充质干细胞，反复贴壁进行纯化；用尼龙棉柱法纯化异体外周血T淋巴细胞。观察骨髓间充质干细胞及其条件培养液体对植物血凝素刺激下T淋巴细胞增殖的影响，以ELISA方法检测γ-干扰素、白细胞介素4水平变化。
结果与结论：骨髓间充质干细胞及其条件培养液体对植物血凝素诱导的T淋巴细胞增殖均有抑制作用，并呈剂量和浓度依赖性。ELISA结果表明，骨髓间充质干细胞及其条件培养液抑制T淋巴细胞γ-干扰素的分泌；骨髓间充质干细胞可促进白细胞介素4的分泌，但其条件培养液对白细胞介素4的分泌无影响。提示骨髓间充质干细胞及其条件培养液对异体外周血T淋巴细胞的增殖有抑制作用，其作用可能与其影响T淋巴细胞分泌γ-干扰素和白细胞介素4有关。

关键词: 免疫调节, 骨髓间充质干细胞, T淋巴细胞, γ-干扰素, 白细胞介素4

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are characterized by supporting hematopoiesis, pluripotential differentiation and immunoregulation. The immunoregulatory effects are of potential importance in prevention and treatment of graft-versus-host disease (GVHD) after transplantation of allogenous hematopoietic stem cells, but the mechanism of immuno-regulation remains unknown.
OBJECTIVE: To investigate the immunoregulatory effects of human BMSCs and their conditioned medium on the secretary function of allogenous peripheral blood T lymphocytes in vitro.
METHODS: BMSCs were isolated from human bone marrow and purified by repeated adherence. Allogenous peripheral blood T lymphocytes were harvested using nylon column. The effects of BMSCs and their conditioned medium on the proliferation of phytohemagglutinin-primed T lymphocytes were observed. Interferon-γ and interleukin 4 levels were detected by ELISA.
RESULTS AND CONCLUSION: BMSCs and their conditioned medium inhibited the proliferation of phytohemagglutinin-primed T lymphocytes in a dose- and concentration-dependent manner. ELISA results showed that BMSCs and their conditioned medium inhibited the secretion of interferon-γ by T lymphocytes. BMSCs could promote the secretion of interleukin-4, but their conditioned medium did not influence the secretion of interleukin-4. These findings suggest that BMSCs and their conditioned medium inhibit the proliferation of allogenous peripheral blood T lymphocytes, and the inhibitory effects may be related to a fact that BMSCs and their conditioned medium influence T lymphocytes to secrete interferon-γ and interleukin-4.

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