中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (21): 3915-3918.doi: 10.3969/j.issn.1673-8225.2012.21.027

• 组织工程口腔材料 tissue-engineered oral materials • 上一篇    下一篇

四种烤瓷冠基底金属浸提液干预人牙龈成纤维细胞尿纤溶酶原激活剂和Ⅰ型纤溶酶原激活物抑制剂的表达★
 

芦  琳，黄克强，胡  静，杜  雅   

  1. 辽宁医学院附属第二医院正畸科，辽宁省锦州市  121004
  • 收稿日期:2012-01-29 修回日期:2012-03-17 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 黄克强，硕士，教授，辽宁医学院附属第二医院正畸科，辽宁省锦州市 121004 hkq9@163.com
  • 作者简介:芦琳★，女，1985年生，河北省石家庄市人，汉族，辽宁医学院在读硕士，主要从事口腔修复的研究。 maomaoyijia@126.com

Effects of four kinds of base metal alloy crown extract on expression levels of urokinase-type plasminogen activator and plasminogen activator inhibitor-1 in human gingival fibroblasts 

Lu Lin, Huang Ke-qiang, Hu Jing, Du Ya   

  1. Department of Orthodontics, Second Affiliated Hospital of Liaoning Medical University, Jinzhou  121004, Liaoning Province, China
  • Received:2012-01-29 Revised:2012-03-17 Online:2012-05-20 Published:2012-05-20
  • Contact: Huang Ke-qiang, Master, Professor, Department of Orthodontics, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121004, Liaoning Province, China hkq9@163.com
  • About author:Lu Lin★, Studying for master’s degree, Department of Orthodontics, Second Affiliated Hospital of Liaoning Medical University, Jinzhou 121004, Liaoning Province, China maomaoyijia@126.com

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摘要：

背景：修复材料的生物相容性是决定临床修复效果的重要因素之一。
目的：通过比较4种金属浸提液对人牙龈成纤维细胞尿纤溶酶原激活剂和Ⅰ型纤溶酶原激活物抑制剂表达的差异探索其生物相容性。
方法：选用活体牙龈，原代培养人牙龈成纤维细胞，采用镍铬、钴铬、纯钛及金钯合金4种金属浸提液进行干预，以未进行干预的细胞作为对照。
结果与结论：ELISA检测结果显示镍铬和钴铬合金浸提液干预后细胞尿纤溶酶原激活剂表达增加；免疫荧光实验结合电镜观察发现镍铬和钴铬合金浸提液干预的细胞胞质荧光染色深，分布均匀，遍布整个细胞，提示细胞Ⅰ型纤溶酶原激活物抑制剂表达增加。而纯钛和金钯合金浸提液对细胞尿纤溶酶原激活剂和Ⅰ型纤溶酶原激活物抑制剂的表达无影响。说明纯钛和金钯合金的生物相容性优于镍铬和钴铬合金。
 

关键词: 金属浸提液, 牙龈成纤维细胞, 尿纤溶酶原激活剂, Ⅰ型纤溶酶原激活物抑制剂, 镍铬合金, 钴铬合金, 纯钛, 金钯合金

Abstract:

BACKGROUND: Biocompatibility of repairing materials is an important factor for clinical effects.
OBJECTIVE: To evaluate the material biocompatibility in oral repair by analyzing different expressional levels of urokinase-type plasminogen activator (uPA) and plasminogen activator inhibitor-1 (PAI-1) of human gingival fibroblasts (HGFs) in four types of metal extractions.
METHODS: Healthy gums were obtained to cultivate HGFs. Levels of PAI-1 and uPA were examined by immunofluorescence and enzyme linked immunosorbent assay (ELISA) detection after different procedures: Co-Cr, Ni-Cr, Ti and rhotanium metal extractions. HGFs with no treatment served as controls.
RESULTS AND CONCLUSION: ELISA results showed an increase in uPA levels after treatment with Co-Cr and Ni-Cr metal extractions. Immunofluorescence detection and electron microscope revealed deep-stained and even-distributed cytoplasm after treatment with Co-Cr and Ni-Cr, indicating PAI-1 levels were increased. There were changes in the levels of uPA and PAI-1 after treatment with Ti and gold alloy metal extractions. Biocompatibility of Ti and rhotanium is better than that of Co-Cr and Ni-Cr.

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