中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (21): 3847-3850.doi: 10.3969/j.issn.1673-8225.2012.21.011

• 复合支架材料 composite scaffold materials • 上一篇    下一篇

不同聚乳酸相对分子质量对其构建复合支架材料生物学功能的影响☆

张  宁，李昀生   

  1. 煤炭总医院口腔科，北京市  100088
  • 收稿日期:2011-10-17 修回日期:2012-01-16 出版日期:2012-05-20 发布日期:2012-05-20
  • 通讯作者: 李昀生，博士，副主任医师，煤炭总医院口腔科，北京市 100088
  • 作者简介:张宁☆，女，1980年生，山西省太原市人，2008年毕业于四川大学，博士，主要从事口腔种植方面的研究。

Comparative study on the different molecule weight of poly-L-lactic acid in the biological function of composite materials☆

Zhang Ning, Li Yun-sheng   

  1. Department of Stomatology, China Meitan General Hospital, Beijing  100088, China
  • Received:2011-10-17 Revised:2012-01-16 Online:2012-05-20 Published:2012-05-20
  • Contact: Li Yun-sheng, Doctor, Associate chief physician, Department of Stomatology, China Meitan General Hospital, Beijing 100088, China zhnivi@hotmail.com
  • About author:Zhang Ning☆, Doctor, Department of Stomatology, China Meitan General Hospital, Beijing 100088, China zhnivi@hotmail.com

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摘要：

架，可以增加成骨细胞的增殖，减少排异反应，提高骨愈合，并具有剂量依赖性。
目的：检测不同聚左乳酸相对分子质量对于聚左乳酸-β-磷酸三钙复合支架材料功能及其结构的影响。
方法：选用相对分子质量为200 000和    380 000的聚左乳酸通过冻干法与β-磷酸三钙制备成聚左乳酸-β-磷酸三钙复合支架材料，检测样本的孔隙率和孔隙直径，将乳兔的骨髓间充质干细胞与相对分子质量为 200 000和380 000的聚左乳酸构建的支架材料复合培养，并与正常培养的乳兔的骨髓间充质干细胞进行形态学、细胞增殖以及细胞中碱性磷酸酶表达水平的对比观察。
结果与结论：电镜结果显示，兔的骨髓间充质干细胞均能在相对分子质量为    200 000和380 000聚左乳酸构建的支架材料表面形成很好的黏附。MTT检测显示各组细胞培养1~7 d时细胞增殖差异没有显著性意义(P > 0.05)，且各组细胞碱性磷酸酶表达水平接近(P > 0.05)。说明含有不同相对分子质量的聚左乳酸-β-磷酸三钙复合支架材料对于兔骨髓间充质干细胞的黏附与增殖无影响。
 

关键词: 聚左乳酸, 复合材料, 高分子聚合材料, 相对分子质量, 骨髓间充质干细胞

Abstract:

BACKGROUND: With the development of technology, poly-L-lactic acid/β-tricalcium phosphate composite materials show good characters in the tissue engineering, which can promote osteoblast proliferation, reduce rejection reactions, and improve bone healing in a dose-dependent manner.
OBJECTIVE: To test the influence of poly-L-lactic acid with different molecule weights on the structure and function of poly-L-lactic acid/β-tricalcium phosphate composite scaffolds.
METHODS: Poly-L-lactic acid with molecule weights of 200 000 and 380 000 were combined with β-tricalcium phosphate to produce composite scaffolds by using freeze-drying method. Porosity and pore size of the samples were measured. The fetal rabbit bone mesenchymal stem cells (BMSCs) were cultured and expanded in vitro. They were harvested and seeded into the prepared poly-L-lactic acid/β-tricalcium phosphate scaffolds. The 3-(4, 5-Dimethylthiazol-2yl)-2, 5 diphenyltetrazolium bromide (MTT) and alkaline phosphatase were examined for comparison between normal cultured BMSCs and those cultured on the different poly-L-lactic acid/β-tricalcium phosphate scaffolds.
RESULTS AND CONCLUSION: Images of scanning electron microscope showed that the cells adhered to the scaffolds greatly. The value of MTT and alkaline phosphatase showed no significant difference (P > 0.05). The molecule weight of poly-L-lactic acid has no influence on the biological function of composite materials.

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