中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (12): 2197-2202.doi: 10.3969/j.issn.1673-8225.2012.12.025

• 生物材料基础实验 basic experiments of biomaterials • 上一篇    下一篇

丝素蛋白/壳聚糖三维多孔支架的构建及结构表征**★

柳  磊1，曾曙光1，任  力2，魏柳琼1，杨  静1，曹彦南1，石  勇1   

  1. 1南方医科大学附属口腔医院，广东省口腔医院，广东省广州市    510280；2华南理工大学材料科学与工程学院，广东省广州市    540641
  • 收稿日期:2011-12-16 修回日期:2012-01-14 出版日期:2012-03-18 发布日期:2012-03-18
  • 通讯作者: 通讯作者：曾曙光，硕士生导师，教授，主任医师，广东省口腔医院，广东省广州市 510280 sunrisez@live.cn 通讯作者：任力，博士，教授，博士生导师，华南理工大学材料科学与工程学院，广东省广州市 540641 psliren@ scut.edu.cn
  • 作者简介:柳磊★，女，1984年生，安徽省界首市人，汉族，南方医科大学在读硕士，主要从事组织工程支架材料的研究。liuleijinhua@ 163.com
  • 基金资助:
    广东省中医药局科研基金资助项目(20111145)；广东省科技计划项目(2011B031300022)。
     

Preparation and characterization of porous silk fibroin/chitosan scaffolds

Liu Lei1, Zeng Shu-guang1, Ren Li2, Wei Liu-qiong1, Yang Jing1, Cao Yan-nan1, Shi Yong1   

  1. 1Affiliated Stomatological Hospital of Southern Medical University, Guangdong Provincial Stomatological Hospital, Guangzhou  510280, Guangdong Province, China; 2Department of Materials Science and Engineering, South China University of Technology, Guangzhou  510641, Guangdong Province, China
  • Received:2011-12-16 Revised:2012-01-14 Online:2012-03-18 Published:2012-03-18
  • Contact: Corresponding author: Zeng Shu-guang, Master’s supervisor, Professor, Chief physician, Affiliated Stomatological Hospital of Southern Medical University, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, Chinasunrisez@live.cn Corresponding author: Ren Li, Doctor, Professor, Doctoral supervisor, Department of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, Guangdong Province, Chinapsliren@scut.edu.cn
  • About author:Liu Lei★, Studying for master’s degree, Affiliated Stomatological Hospital of Southern Medical University, Guangdong Provincial Stomatological Hospital, Guangzhou 510280, Guangdong Province, China liuleijinhua@163.com
  • Supported by:

    Research Foundation of Guangdong Bureau of Traditional Chinese Medicine, No. 20111145*; Guangdong Science and Technology Program, No.2011B031300022*

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摘要：

背景：丝素蛋白和壳聚糖均无毒性且具有良好的生物相容性，但是单一成分作为生物支架时都不能满足支架材料的需求。
目的：制备各种不同组分的丝素蛋白及壳聚糖复合支架材料，观察其微观结构及相关性能，筛选出适合成骨细胞生长的理想支架材料。
方法：通过CaCl2∶C2H5OH∶H2O=1∶2∶8(摩尔比)溶解体系溶解、过滤、浓缩提纯，制备出2%的丝素蛋白溶液，壳聚糖溶解于乙酸溶液配制成的3%壳聚糖溶液，将两者以不同的比例相混合，经数次冷冻干燥后，得到成品支架材料。采用电镜观察形貌，计算孔隙率并对支架的结构进行红外、X射线衍射、电子能谱分析观察。
结果与结论：将壳聚糖和丝素蛋白共混后，互为改性，制备出了结构较稳定的支架材料。其中40%丝素蛋白-60%壳聚糖组具有适合成骨细胞生长的较佳孔径，可作为细胞支架的首选配比。
关键词：丝素蛋白；壳聚糖；骨组织工程；支架；生物材料
doi:10.3969/j.issn.1673-8225.2012.12.025

关键词: 丝素蛋白, 壳聚糖, 骨组织工程, 支架, 生物材料

Abstract:

BACKGROUND: Silk fibroin and chitosan are non-toxic and have a good histocompatibility. However, they could not satisfy the biological scaffold material needs if working separately.
OBJECTIVE: To explore and analyze the material structure and relate performance for characterization on the basis of making a variety of compound scaffolds with different proportion of silk fibroin and chitosan, and to select the ideal scaffolds that suitable for the growth of osteoblasts.
METHODS: 2% silk fibroin solution was made by dissolution from CaCl2 solvent systems at a mole ratio of CaCl2:C2H5OH:H2O=  1:2:8, filtration, concentration and purification. The chitosan was dissolved in acetic acid solution prepared 3% chitosan-acetic acid solution. The silk fibroin and chitosan were blend in a freeze-drying approach in different proportion in order to get different scaffold materials. Then the morphology of the silk fibroin/chitosan scaffolds was observed under a scanning electron microscope; the porosity was calculated and the structure of the scaffolds was evaluated and observed by Fourier transform infrared spectroscopy, X-ray diffraction and electron spectroscopy.
RESULTS AND CONCLUSIONS: More stable scaffolds were created with the method of blending and modifying silk fibroin and chitosan. The 40% silk fibroin-60% chitosan group proves the preferred ratio of cell scaffold material due to its better aperture for the growth of osteoblasts.

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