中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (10): 1716-1720.doi: 10.3969/j.issn.1673-8225.2012.10.002

• 骨髓干细胞 bone marrow stem cells • 上一篇    下一篇

氯甲基苯甲酰氨标记骨髓间充质干细胞在免疫介导骨髓衰竭小鼠模型体内的归巢★

王  洋1，2，肖  扬2，李  力2，蒋祖军2，李勇华2，庞  妍2，宋佳音2   

  1. 1南方医科大学，广东省广州市 510515；2解放军广州军区广州总医院血液科，广东省广州市 510010
  • 收稿日期:2011-11-30 修回日期:2012-01-29 出版日期:2012-03-04 发布日期:2012-03-04
  • 通讯作者: 肖扬，硕士生导师，主任医师，解放军广州军区广州总医院血液科，广东省广州市 510010 jdxiao111@163.com
  • 作者简介:王洋★，女，1986 年生，吉林省蛟河市人，汉族，南方医科大学在读硕士，主要从事骨髓间充质干细胞在再生障碍性贫血模型鼠体内归巢机制的研究。407853954@qq.com

In vivo homing of chloromethyl-benzamidodialkylcarbocyanine-labeled bone marrow mesenchymal stem cells after transplantation into a mouse model of immune-mediated bone marrow failure

Wang Yang1, 2, Xiao Yang2, Li Li2, Jiang Zu-jun2, Li Yong-hua2, Pang Yan2, Song Jia-yin2   

  1. 1Southern Medical University, Guangzhou  510515, Guangdong Province, China; 2Hematology Department, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou  510010, Guangdong Province, China
  • Received:2011-11-30 Revised:2012-01-29 Online:2012-03-04 Published:2012-03-04
  • Contact: author: Xiao Yang, Master’s supervisor, Chief physician, Hematology Department, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, Guangdong Province, China jdxiao111@163.com
  • About author:Wang Yang★, Studying for master’s degree, Southern Medical University, Guangzhou 510515, Guangdong Province, China; Hematology Department, Guangzhou General Hospital of Guangzhou Military Command, Guangzhou 510010, Guangdong Province, China 407853954@qq.com

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摘要：

背景：再生障碍性贫血是T淋巴细胞免疫亢进破坏造血干祖细胞的一种异常免疫反应性疾病。骨髓间充质干细胞具有支持造血功能同时具有免疫调控作用。
目的：观察骨髓间充质干细胞移植骨髓衰竭模型小鼠体内的归巢情况。
方法：将BALB/c小鼠随机分成正常对照组、骨髓衰竭模型组和骨髓间充质干细胞移植组，于第6天将已标记氯甲基苯甲酰氨的BALB/c小鼠骨髓间充质干细胞经尾静脉注射途径移植给骨髓衰竭模型小鼠，采用流式细胞术和组织学荧光显微镜观察标记细胞在不同组织的动态分布。造模第14 天，观察各组小鼠的平均生存时间、外周血血象和骨髓形态学特征。
结果与结论：骨髓间充质干细胞经尾静脉输注后24 h在受体小鼠骨髓中可发现氯甲基苯甲酰氨阳性标记细胞，72 h时阳性标记细胞数量增多(P < 0.05)。骨髓间充质干细胞移植组小鼠濒死或处死前的白细胞、血红蛋白、血小板、骨髓单个核细胞数与模型组小鼠濒死时相比显著升高(P < 0.01)。骨髓衰竭模型组比骨髓间充质干细胞移植组平均生存时间短(P < 0.05)。结果证实，骨髓间充质干细胞输入骨髓衰竭模型体内能归巢至骨髓，促进造血恢复，减轻骨髓衰竭程度，显著延长生存时间。  

关键词: 骨髓衰竭, 氯甲基苯甲酰氨, 骨髓间充质干细胞, 再生障碍性贫血, 归巢, 流式细胞术, 细胞示踪, 干细胞培养

Abstract:

BACKGROUND: Aplastic anemia (AA) is characterized by pancytopenia and bone marrow (BM) hypoplasia. In most cases, AA is an immune-mediated disease, with active destruction of hematopoietic stem and progenitor cells by T lymphocytes. Bone marrow mesenchymal stem cells (BMSCs) can support hematopoietic cells and have immunomodulatory effects.
OBJECTIVE: To observe the homing of BMSCs transferred into mouse model of bone marrow failure.
METHODS: BALB/C mice were randomly divided into normal control group, AA group and bone marrow mesenchymal stem cell transplantation group. On day 6, chloromethyl-benzamidodialkylcarbocyanine (CM-Dil)-labeled BALB/c mouse bone marrow mesenchymal stem cells were transplanted into mouse model of bone marrow failure via tail vein. The dynamic distribution of CM-Dil-labeled cells in different tissues was observed by flow cytometry and fluorescence microscope. On day 14 after bone marrow failure induction, the average survival time, peripheral blood hemogram and the morphological features of bone marrow of mice in each group were determined.
RESULTS AND CONCLUSION: At 24 hours after BMSC transplantation via tail vein, CM-Dil-labeled positive cells were observed in recipients’ bone marrow, and the CM-Dil-labeled positive cells increased at 72 hours (P < 0.05). The white blood cells, hemoglobin, platelets and bone marrow mononuclear cells of the dying or executed mice in the transplantation group were significantly higher than those in the aplastic anemia group (P < 0.01). The average survival time of mice in aplastic anemia group was significantly shorter than that in the BMSC transplantation group (P < 0.05). These findings suggest that the transplanted BMSCs can home into the bone marrow of bone marrow failure model, promote the recovery of hematopoietic function, alleviate the degree of bone marrow failure, and significantly prolong survival time. 
 

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