中国组织工程研究

中国组织工程研究 ›› 2012, Vol. 16 ›› Issue (7): 1215-1219.doi: 10.3969/j.issn.1673-8225.2012.07.018

• 组织构建细胞学实验 cytology experiments in tissue construction • 上一篇    下一篇

运动条件下健康人循环内皮祖细胞的数量和功能*****☆

杨  震1，赖光华1，夏文豪1，罗初凡2，程  飞1，廖艳红1，王洁梅1， 陈  龙1，廖新学1，靳亚非1 ，陶  军1    

  1. 中山大学附属第一医院，1高血压血管病科，2心血管介入病区，广东省广州市  510080
  • 收稿日期:2011-08-06 修回日期:2011-11-09 出版日期:2012-02-12 发布日期:2012-02-12
  • 通讯作者: 陶军，博士，教授，博士生导师，中山大学附属第一医院高血压血管病科，广东省广州市 510080 taojungz@yahoo.com
  • 作者简介:杨震☆，男，1978年生，湖南省衡阳市人，汉族，博士，2007年中山大学毕业，主治医师，主要从事心血管内科方面的研究。 yangzhen107107@yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目(u0732002, 30800215, 30770895)，广东省自然科学基金博士启动项目(8451008901000793)，广州市科技计划(2007Z3-E0241)

Effect of exercise on number and activity of circulating endothelial progenitor cells in healthy subjects

Yang Zhen1, Lai Guang-hua1, Xia Wen-hao1, Luo Chu-fan2, Cheng Fei1, Liao Yan-hong1, Wang Jie-mei1, Chen Long1, Liao Xin-xue1, Jin Ya-fei1, Tao Jun1   

  1. 1Department of Hypertension & Vascular Disease, 2Department of Cardiac Intervention, First Affiliated Hospital of Sun Yat-sen University, Guangzhou  510080, Guangdong Province, China
  • Received:2011-08-06 Revised:2011-11-09 Online:2012-02-12 Published:2012-02-12
  • Contact: Tao Jun, Doctor, Professor, Doctoral supervisor, Department of Hypertension & Vascular Disease, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China taojungz@yahoo.com
  • About author:Yang Zhen☆, Doctor, Attending physician, Department of Hypertension & Vascular Disease, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China yangzhen107107@yahoo.com.cn
  • Supported by:

    National Natural Science Foundation of China, No. u0732002*, 30800215*, 30770895*; Doctor Starting Project of Natural Science Foundation of Guangdong Province, No. 8451008901000793*; Science and Technology Project of Guangzhou, No. 2007Z3-E0241*

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摘要：

背景：运动条件对内皮祖细胞数量和功能是否会产生的影响目前尚无公识。
目的：观察急性运动对健康成人循环内皮祖细胞数量和功能的影响。
方法：健康男性成人志愿者12例参加急性平板运动锻炼(9.3±2.1) min。用流式细胞仪测定运动前后CD34和KDR双标阳性循环内皮祖细胞水平，ac-LDL及lectin荧光标记方法评估体外培养内皮祖细数量，检测内皮祖细胞的黏附、迁移和增殖能力，并测定运动前后血浆和内皮祖细胞分泌一氧化氮、血管内皮生长因子和粒-巨噬细胞集落刺激因子水平变化。
结果与结论：流式细胞仪检测显示健康志愿者运动后循环内皮祖细胞水平较运动前增加(P < 0.05)。荧光标记法证实运动后ac-LDL及lectin抗体双阳性内皮祖细胞数量较运动前增加(P < 0.05)，内皮祖细胞迁移和增殖能力明显增强(P < 0.05)，但黏附能力无明显变化。急性运动能明显增加健康志愿者的血浆一氧化氮水平(P < 0.05)，健康志愿者运动前后血浆一氧化氮水平和循环内皮祖细胞数量及功能的增加倍数呈明显的直线相关回归关系(P < 0.05)。结果证实，运动可明显增加健康成人循环内皮祖细胞的数量和功能，其机制与其诱导一氧化氮释放增多有关。

关键词: 内皮祖细胞, 运动, 内皮功能, 心血管疾病, 组织工程

Abstract:

BACKGROUND: Whether exercise can influence the number and activity of endothelial progenitor cells (EPCs) is still unclear.
OBJECTIVE: To observe the effect of exercise on number and activity of circulating EPCs in healthy subjects.
METHODS: Twelve male healthy volunteers underwent acute dynamic treadmill exercise with (9.3±2.1) minutes. Flow cytometry analysis was used to evaluate the number of CD34 and KDR double-positive labeled circulating EPCs before and after exercise, and acetylated-low-density lipoprotein (LDL) and lectin fluorescent staining method were used to evaluate the number of cultured EPCs. The adhesion, migration and proliferation of EPCs were detected. In addition, the nitric oxide (NO), vascular endothelial growth factor (VEGF) and granulocyte-macrophage colony stimulating factor (GM-CSF) levels in plasma and secreted by circulating EPCs were measured before and after exercise.
RESULTS AND CONCLUSION: Flow cytometry analysis showed that the number of circulating EPCs increased after exercise   (P < 0.05). The acetylated-LDL and lectin fluorescent staining method indicated that the cultured EPCs also increased after exercise (P < 0.05). The proliferative and migratory activities of cultured EPCs were higher after exercise compared with before exercise (P < 0.05), but the adhesive activity did not change. Acute exercise also significantly enhanced plasma NO levels in healthy subjects (P < 0.05). There was a significant linear regression relationship between the enhanced plasma NO level and increased number or activity of circulating EPCs in healthy subjects (P < 0.05). Exercise can increase the number and activity of circulating EPCs in healthy subjects, which are related to the enhanced NO production in healthy subjects.

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